Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。     

Correspondence between the large volcanic eruptions and ENSO events over AD 1525–2000

ENSO is an interannual mode which may be affected by external forcing, such as volcanic eruptions. Based on the reconstructed volcanic eruptions chronology and ENSO sequences, both 195 large volcanic eruptions(VEI≥4) and 398 ENSO(El Ni?o and La Ni?a) events were extracted from 1525 to 2000. An analysis of the correspondence between the large volcanic eruptions and ENSO events was performed by matching the large volcanic eruptions with the types and magnitudes of ENSO events present in the 0–2 years after the eruptions. The results show the following:(1) The percentages of ENSO events within the 3 years after the large eruptions had increased to 68.3% from 31.7% compared with those with no-eruptions in the previous 0–2 years. In addition, the ratio of El Ni?o to La Ni?a events turned from 2:3 to 1:1, and more El Ni?o events occurred in the 0 year after eruptions in the low-latitudes of the Northern Hemisphere and in the tropics but more La Ni?a events occurred in the 0 year after in the high-latitudes of the Northern Hemisphere and the Southern Hemisphere.(2) After the eruptions, the weak(W) El Ni?o events had increased by 8 percentage points and the very strong(VS) El Ni?o events had decreased by 10 percentage points; conversely, there was a decrease by 15 percentage points of the weak La Ni?a events and an increase by 11.4 percentage points of the very strong La Ni?a events. Specifically, the percentages of strong La Ni?a events increased to a peak at 1(+1) year after the eruptions.(3) The percentage of eruptions followed by single-year ENSO was the greatest. The percentage of ENSO events that occurred in the consecutive 2 years following an eruption was approximately equal to the percentage of events that occurred consecutively 3 years following an eruption, and both sets of ENSO magnitudes showed a decreasing trend.     

Effect of farmland expansion on drought over the past century in Songnen Plain,Northeast China

The effects of human activities on climate change are a significant area of research in the field of global environmental change. Land use and land cover change(LUCC) has a greater effect on climate than greenhouse gases, and the effect of farmland expansion on regional drought is particularly important. From the 1910 s to the 2010 s, cultivated land in Songnen Plain increased by 2.67 times, the area of cultivated land increased from 4.92×10~4 km~2 to 13.14×10~4 km~2, and its percentage of all land increased from 25% to 70%. This provides an opportunity to study the effects of the conversion of natural grassland to farmland on climate. In this study, the drought indices in Songnen Plain were evaluated from the 1910 s to the 2010 s, and the effect of farmland expansion on drought was investigated using statistical methods and the Weather Research and Forecasting Model based on UK's Climatic Research Unit data. The resulting dryness index, Palmer drought severity index, and standardized precipitation index values indicated a significant drying trend in the study area from 1981 to 2010. This trend can be attributed to increases in maximum temperature and diurnal temperature range, which increased the degree of drought. Based on statistical analysis and simulation, the maximum temperature, diurnal temperature range, and sensible heat flux increased during the growing season in Songnen Plain over the past 100 years, while the minimum temperature and latent heat flux decreased. The findings indicate that farmland expansion caused a drying trend in Songnen Plain during the study period.     

城镇化与生态环境耦合的动态模拟——研究进展与展望

Urbanization and eco-environment coupling is a research hotspot.Dynamic simulation of urbanization and eco-environment coupling needs to be improved because the processes of coupling are complex and statistical methods are limited.Systems science and cross-scale coupling allow us to define the coupled urbanization and eco-environment system as an open complex giant system with multiple feedback loops.We review the current state of dynamic simulation of urbanization and eco-environment coupling and find that:(1)The use of dynamic simulation is an increasing trend,the relevant theory is being developed,and modeling processes are being improved;(2)Dynamic simulation technology has become diversified,refined,intelligent and integrated;(3)Simulation is mainly performed for three aspects of the coupling,multiple regions and multiple elements,local coupling and telecoupling,and regional synergy.However,we also found some shortcomings:(1)Basic theories are inadequately developed and insufficiently integrated;(2)The methods of unifying systems and sharing data are behind the times;(3)Coupling relations and the dynamic characteristics of the main driving elements are not fully understood or completely identified.Additionally,simulation of telecoupling does not quantify parameters and is not systemically unified,and therefore cannot be used to represent spatial synergy.In the future,we must promote communication between research networks,technology integration and data sharing to identify the processes governing change in coupled relations and in the main driving elements in urban agglomerations.Finally,we must build decision support systems to plan and ensure regional sustainable urbanization.     

Impact of Reservoir Permeability,Permeability Anisotropy and Designed Injection Rate on CO2 Gas Behavior in the Shallow Saline Aquifer at the CaMI Field Research Station,Brooks, Alberta

Natural Resources Research - Carbon capture and storage is part of Canada’s climate change action plan to reduce greenhouse gas emissions. The Containment and Monitoring Institute Field...     

Prediction of Blast-Induced Rock Movement During Bench Blasting: Use of Gray Wolf Optimizer and Support Vector Regression

Natural Resources Research - A large ore loss and dilution can be expected when using a pre-blast ore boundary for shovel guidance because of the movement and re-distribution of ore in the muck...     

基于生态足迹的格网化生态承载力评价——以衡水市为例

针对行政单元生态承载力评价不能精细反映区域内部生态状况的差异和局部细节特征的问题,难以满足精细管控生态承载力的需要,本文提出了格网化生态承载力评价方法。基于生态足迹模型,结合人口空间分布模型,借助GIS空间分析技术,从格网角度对生态足迹和生态承载力进行定量估算与空间格局分析,并以衡水市为例进行试验验证。结果表明,格网尺度生态承载力评价方法与区域生态承载力评价方法相比更符合真实情况。从格网尺度看,衡水市生态足迹分布圈层特点,生态承载力呈离散分布特征。生态赤字状况表现出明显的异质性,沿滏东排河和滏阳河两侧呈条带状分布。衡水市大部分地区处于生态赤字临界状态,生态状况亟须得到保护。     

青岛市生态环境变化遥感监测与分析

城市化进程不断加快带来一系列生态环境问题,本文利用植被指数、湿度指数、地表温度、建筑物—裸土指数定量表征绿度、湿度、热度、干度4个生态要素指标,通过主成分分析法,建立遥感生态指数模型,并从时间和空间两个维度对比分析2013、2019年两个时期遥感生态指数。结果表明,青岛市生态环境呈局部优化改进、整体下降趋势;同时,将青岛市遥感生态指数与人类活动相关的地表覆盖变化数据、路网交通数据、夜光数据进行耦合性分析,进一步分析探寻生态环境变化的影响因子。     

规则池塘群的复杂直线模式识别

针对现有面要素直线模式识别方法难以解决规则池塘中破碎区域的问题,提出了一种规则池塘群的复杂直线模式识别方法。首先,分析了规则池塘群的空间特征和认知特点,提出了“主次关系→并列关系→直线模式→复杂直线模式”的多层次认知顺序;其次,设计了主次关系池塘组、并列关系池塘组、直线模式池塘群的识别方法;最后,构建了复杂直线模式池塘群的识别模型。试验表明:本文方法能够消除破碎区域对规则池塘群直线模式提取的不利影响,有效提升复杂直线模式识别的质量。     

PTP协议在广电时间同步网中的应用研究

随着广电行业的飞速发展,以串行数字接口(SDI)基带信号为基础技术架构的传统电视信号已经很难满足未来技术发展和业务扩展的需求．得益于现代通信技术的迅速发展以及即将到来的万物互联的5G时代,广电行业也将采用IP化技术来构建新的网络系统．处在向IP化发展的过渡期,广电IP化目前还存在一些亟待解决的问题．为了解决广电系统网络中需要更高精度的同步信号来完成时间同步的问题,本文利用精确时间协议（PTP）时间同步技术在专用以太网中完成时间同步组网,搭建了测试环境并对测试数据进行了分析．测试结果表明,相较于传统的网络时间协议（NTP）时间同步技术,输出PTP信号的准确度能够达到亚微秒量级,完全满足广电行业IP化数字电视系统时间同步信号的高精度需求．