Cloning of cytochrome P450 1A (CYP1A) genes from the hermaphrodite fish Rivulus marmoratus and the Japanese medaka Oryzias latipes

To use two small fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae) and the Japanese medaka Oryzias latipes (Belloniformes) as testing models in molecular ecotoxicology, we have cloned the cytochrome P450 1A (CYP1A) gene after screening of both genomic DNA libraries, and sequenced 11,863 and 7,243 bp including all the exons and introns with promoter regions, respectively. The Rivulus and the medaka CYP1A gene consisted of seven exons (including non-coding exons) with high homology to mammals. In the promoter region, Rivulus CYP1A gene has seven xenobiotic response elements (XREs) and two metal response elements (MREs), while the Japanese medaka CYP1A gene has six XREs and four MREs. Interestingly, medaka CYP1A gene has a number of MREs at the promoter, which may affect its response on metal exposure. We describe here the gene structure of both fish CYP1A genes.     

Glaciomarine sedimentation and palaeo-glacial setting of Maxwell Bay and its tributary embayment, Marian Cove, South Shetland Islands, West Antarctica

High-resolution (3.5 kHz and multi-channel) seismic profiles and piston cores were collected from Maxwell Bay and its tributary embayment, Marian Cove, in the South Shetland Islands, Antarctica, during the Korea Antarctic Research Program (1992/93 and 1995/96) to elucidate the glaciomarine sedimentation processes and recent glacial history of the area. Seismic data from Maxwell Bay reveal a rugged bay margin and flattened basin floor covered with well-stratified hemipelagic muds. On the base-of-slope, acoustically transparent debris flows occur, indicating downslope resedimentation of glaciomarine sediments. Despite the subpolar and ice-proximal settings of Marian Cove, the seafloor is highly rugged with a thin sediment drape, suggesting that much of the area has been recently eroded by glaciers. Sediment cores from the cove penetrated three distinct fining-upward lithofacies: (1) basal till in the lower part of the core, accumulated just seaward of the grounding line of the tidewater glacier; (2) interlaminated sand and mud in the middle part, deposited in ice-proximal zone by a combination of episodic subglacial meltwater inflow and iceberg dumping; and (3) pebbly mud in the upper part, deposited in ice-distal zone by both surface meltwater plume and ice-rafting from the glacier front. A reconstruction of the glacial history of these areas since the late glacial maximum shows an ice sheet filling Maxwell Bay in late Wisconsin time and grounding of the tidewater glacier in Marian Cove until about 1300 yr BP.     

On the recovery of surface wave by pressure transfer function

Except the commonly selected pressure transfer function derived from the linear wave theory, a previous study on the pressure transfer function for recovering surface wave from underwater pressure transducer suggested that the pressure transfer function is a function of frequency parameter only. With careful analysis, this study showed that the pressure transfer function should include a transducer submergence parameter as that given by the linear theory. It was found that the previously suggested empirical formula should be restricted to measurements with the pressure transducer close to the surface; otherwise overestimation of wave height would result. Field measurements were carried out with an acoustic wave gauge and a synchronized pressure transducer located at various depths with submergence parameter close to 1 (near the sea floor). It was shown that the previous one-parameter empirical formula might overestimate the significant wave height by more than 30%. This study found that with deep-water wave bursts excluded, the transfer function based on the linear wave theory provided a fairly good estimation on the significant wave heights, with an average deviation of 3.6%.     

Consumption of bacteria by larvae of a deep-sea polychaete

We investigated whether trochophore larvae of the polychaete Hesiocaeca methanicola, which lives on exposed ice‐like methane hydrates between 500 and 600 m, could consume near‐bottom picoplankton. In laboratory trials larvae significantly reduced the growth rates of all types of picoplankton, including heterotrophic bacteria, Prochlorococcus sp., Synechococcus‐type cyanobacteria and phototrophic eucaryotes <3 μm. Our findings suggest that these types of plankton may be important food sources for deep‐sea planktotrophic larvae.     

Mapping sediment acoustic impedance using remote sensing acoustic techniques in a shallow-water carbonate environment

 Real-time trackline plots of surficial sediment acoustic impedance delineate several sedimentary facies off Garden Key in the Dry Tortugas. The sea floor within a 6×6 km surveyed area consists of carbonate muds (silts), sands and shell, rock, and live corals. The 4-kHz acoustic data supports this finding by providing a pictographic representation of the distribution and structure of several sediment facies types. Plotting the gridded acoustic data with commercial mapping software (Surfer) provides a three-dimensional (3D) perspective of the bottom topography with a color contour map of surficial sediment impedance (upper 0.4 m) draped over the 3D surface.     

Evaluation of bending moments and shear forces at unit connections of very large floating structures using hydroelastic and rigid body analyses

This paper investigates the characteristics of bending moments, shear forces and stresses at unit connections of very large floating structures (VLFS) under wave loads. The responses of VLFS are calculated by solving multi-body motion equation considering hydroelasticity and connection stiffness. Hydroelastic responses are calculated by the direct method. Higher-order boundary element method (HOBEM) is used for fluid analysis and finite element method (FEM) is introduced for structural analysis. The equation of motion is modified to describe the unit connections by employing spring elements. Bending moments and shear forces at the connections are obtained from the dynamic equilibrium condition for pressures and inertia forces. Two types of VLFS units such as tandem arranged units and side-by-side arranged units are considered in the numerical examples. The influences of connection stiffness, wave frequency and heading angle on responses of VLFS are investigated through the numerical examples. Rigid body analysis along with hydroelastic analysis is also carried out in the numerical analysis and comparison of those two approaches is discussed.     

Ultimate strength of ship hulls under torsion

For a ship hull with large deck openings such as container vessels and some large bulk carriers, the analysis of warping stresses and hatch opening deformations is an essential part of ship structural analyses. It is thus of importance to better understand the ultimate torsional strength characteristics of ships with large hatch openings. The primary aim of the present study is to investigate the ultimate strength characteristics of ship hulls with large hatch openings under torsion. Axial (warping) as well as shear stresses are normally developed for thin-walled beams with open cross sections subjected to torsion. A procedure for calculating these stresses is briefly described. As an illustrative example, the distribution and magnitude of warping and shear stresses for a typical container vessel hull cross section under unit torsion is calculated by the procedure. By theoretical and numerical analyses, it is shown that the influence of torsion induced warping stresses on the ultimate hull girder bending strength is small for ductile hull materials while torsion induced shear stresses will of course reduce the ship hull ultimate bending moment.