Construction of bacterial artificial chromosome libraries for Zhikong Scallop <Emphasis Type="Italic">Chlamys farreri</Emphasis>

Two Large-insert genomic bacterial artificial chromosome （BAC） libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I, respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb, providing a coverage of 5.3 haploid genome equivalents. Similarly, the Mbo I library with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 haploid genome equivalents.     

Pb distribution and translocation in Jiaozhou Bay

The trends of distribution, translocation and seasonal change of heavy metal Pb were studied based on the surface and bottom water sampling in Jiaozhou Bay in 1979, and compared with those in 1990＇s. The results showed that the source of Pb in the bay was from wastewater and sewage in the east of Jiaozhou Bay from ocean vessels. Pb concentration was higher in spring and lower in summer and autumn, and remained stable through sedimentation in the bottom layer. The overall water quality was good in 1970＇s. Compared with the environmental monitoring data of 1995-1999, Pb pollution had become serious. Therefore, more efforts should be made to protect the bay from Pb pollution.     

Rossby waves with linear topography in barotropic fluids

Rossby waves are the most important waves in the atmosphere and ocean, and are parts of a large-scale system in fluid. The theory and observation show that, they satisfy quasi-geostrophic and quasi-static equilibrium approximations. In this paper, solitary Rossby waves induced by linear topography in barotropic fluids with a shear flow are studied. In order to simplify the problem, the topography is taken as a linear function of latitude variable y, then employing a weakly nonlinear method and a perturbation method, a KdV （Korteweg-de Vries） equation describing evolution of the amplitude of solitary Rossby waves induced by linear topography is derived. The results show that the variation of linear topography can induce the solitary Rossby waves in barotropic fluids with a shear flow, and extend the classical geophysical theory of fluid dynamics.     

The hydrogen peroxide impact on larval settlement and metamorphosis of abalone <Emphasis Type="Italic">Haliotis diversicolor supertexta</Emphasis>

Abalone Haliotis diversicolor supertexta is an important economic mollusk. The settlement and metamorphosis are two critical stages during its development period, which has direct influence on abalone survival and production. The influence of reactive oxygen species （hydrogen peroxide） on abalone embryo and juvenile development were examined in this study. Larvae of Haliotis diversicolor supertexta were induced to settlement and metamorphose by exposure to seawater supplemented with hydrogen peroxide. They had the best performance at 800 μmol/L. The concentration of 1 000 μmol/L or higher was toxic to the larvae, as the larvae could settle down only at benthic diatom plates without complete metamorphosis. In addition, H2O2 adding time was critical to the larval performance. 24h after two-day post-fertilization was proved to be the optimal adding time. In this paper, two action mechanisms of hydrogen peroxide are discussed： （1） hydrogen peroxide has direct toxicity to ciliated cells, thus cause apoptosis; （2） hydrogen peroxide, as a product from catecholamines＇ autoxidation process in vivo, can reverse this process to produce neuro-transmitters to induce abalone metamorphosis.     

Field detection of chlorophyll-a concentration in the sea surface layer by an airborne oceanographic lidar

An airborne oceanographic lidar, with a frequency-tripled Q-switched Nd： YAG laser of 355 nm, has been designed to measure chlorophyll-a （Chl-a） concentration in the sea surface layer by the Ocean Remote Sensing Institute, OUC. The field experiment was carried out in the bay which is located south of the Liaodong Peninsula on the 10th of September 2005. After the flight, the raw data were processed and analyzed by the fluorescence-to-Raman ratio method with seawater attenuation coefficients calculated from signal profiles. The results of Chl-a concentration sea water were also compared with those of Chl-a concentration by measurements by lidar are shown. The measurements in clear a Moderate Resolution Imaging Spectroradiometer （MODIS）.     

Field Detection of Chlorophyll-a Concentration in the Sea Surface Layer by an Airborne Oceanographic Lidar

An airborne oceanographic lidar, with a frequency-tripled Q-switched Nd: YAG laser of 355 nm, has been designed to measure chlorophyll-a (Chl-a) concentration in the sea surface layer by the Ocean Remote Sensing Institute, OUC. The field experiment was carried out in the bay which is located south of the Liaodong Peninsula on the 10th of September 2005. After the flight, the raw data were processed and analyzed by the fluorescence-to-Raman ratio method with seawater attenuation coefficients calculated from signal profiles. The results of Chl-a concentration measurements by lidar are shown. The measurements in clear sea water were also compared with those of Chl-a concentration by a Moderate Resolution Imaging Spectroradiometer (MODIS).     

Hematological changes in white spot syndrome virus-infected shrimp, Fenneropenaeus chinensis (Osbeck)

The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV (white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifestations of hemocytes were similar among moribund shrimps infected via injection, feeding and by nature. Firstly, the total hemocyte counts (THCs) in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly, necrotic, broken and disintegrated cells were often observed, and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly, necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared, whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV, and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.     

基坑及周边建筑物变形监测方案

结合某基坑变形监测项目,从观测墩及觇牌的设计、观测方法、精度估计等方面对基坑水平位移观测进行了介绍,并介绍了利用差异沉降量推算法进行倾斜观测的方法.