应用实时定量PCR技术检测秦皇岛近岸海域褐潮原因种——抑食金球藻(Aureococcus anophagefferens)

Aureococcus anophagefferens, a small pelagophyte algae, has caused brown tide blooms in coastal waters of Qinhuangdao in recent years, presenting significant negative impacts on the shellfish mariculture industry. Under standard light microscopy, it is visually indistinguishable from other small algae in field samples due to its extremely small size. In this study, quantitative polymerase chain reaction(q PCR) based on 18 S r DNA sequences was developed and used to detect and enumerate A. anophagefferens. A linear regression(R2 = 0.91) was generated based on cycle thresholds value(Ct) versus known concentrations of A. anophagefferens. Twenty-two field samples collected in coastal waters of Qinhuangdao were subjected to DNA extraction and then analyzed using q PCR. Results showed that A. anophagefferens had a wide distribution in coastal waters along Qinhuangdao. Elevated A. anophagefferens abundance, category 3 brown tide blooms(200 000 cells/m L) occurred at Dongshan Beach and Tiger-stone Beach in August in 2013. In shellfish mariculture areas along coastal waters of Qinhuangdao, 4 stations had category 3 blooms, and 6 stations had category 2 blooms(35 000–200 000 cells/m L) in August and all stations had category 1 blooms(0 to ≤35 000 cells/m L) in October. Quantitative PCR allows for detection of A. anophagefferens cells at low levels in filed samples, which is essential to effective management and prediction of brown tide blooms.     

基于迁移学习方法的山东冷云冰晶特征分布研究

为分析冷云中冰晶的分布特征,揭示冰晶增长演变机制,根据冰晶形状和尺度特征分为8类并进行标注,同时标注1类隔断栏进行数据质量控制,将9类标签图像整合并建立图像集,利用迁移学习VGGNet16方法进行识别训练,经训练模型分类准确率达98%。将模型应用到秋季冷云冰晶特征研究中,选取3次积层混合云和3次层状云降水过程,分析冰晶形状在不同温度区间的占比及冰晶谱变化特征,结果表明,温度通过影响冰晶基面与棱面的比值来决定冰晶初始形状分布,相同温度区间积层混合云内球状冰晶和线型冰晶占比高于层状云,低于-12℃后各类冰晶占比相对固定;积层混合云内线型冰晶直径集中在300~800 μm,冰晶谱呈多峰分布,聚合体直径大于600 μm,冰晶谱首尾两端浓度相当,球状冰晶直径集中在120～300 μm,冰晶谱呈单调下降趋势。     

Eutrophication,health risk assessment and spatial analysis of water quality in Gucheng Lake,China

The water characteristics of the Gucheng Lake, such as eutrophication, health and spatial distribution, were investigated. On the basis of the trophic state index (TSI) and entropy weight, a synthesized trophic state index (STSI) model was established to assess lake eutrophication condition through calculating STSI, choosing TP, TN, COD, BOD and NH₃-N as trophic variables. The STSI ranged from 50.58 to 62.44, which showed that the water has been between eutrophic and supereutrophic. A histogram was applied to health risk assessment which was analyzed from carcinogenic substances (Cr⁺⁶, As and Cd) and non-carcinogenic substances (hydroxybenzene, Pb, Hg, CN⁻ and NH₃), and the results showed that the former was much greater than the latter for effect. The total risk for each resident caused by all pollutants ranged from 5.18E-05 to 8.34E-05, which is far higher than the standard, recommended by Sweden Bureau of Environment Protection and Holland Ministry of Building and Environment Protection (1.0E-05). Cluster analysis was used to detect similarities and dissimilarities among the seven sampling sites and explain the observed clustering in terms of affected conditions. Twenty-one variables were used to divide seven sampling sites into three groups, namely, north lake, south lake and lake center.     

滑坡灾害环境与山区建设的和谐

滑坡是浙江省沿海及丘陵山区频发的地质灾害之一,常堵塞交通、农田毁损、民房倒塌、使山区人民生活环境严重恶化。分析滑坡灾害机理,提早预警预报,是合理规划山区民居建设、治理地质灾害、建立民居与生态环境合谐的的关系,是各级政府、地质工程界、建设部门和环境监理部门的重要社会责任和理论研究范围。     

Grain-size features of a Miocene loess-soil sequence at Qinan: Implications on its origin

In northern China, the Quaternary loess-soil se-quences[1] and the Hipparion Red-Earth Formation in the eastern Loess Plateau[2―6] provide a continental climate record for the past 8 Ma. The recently reported Miocene[7] and Pliocene[8] loess-soil sequences near Qinan constitute an eolian record of the western Loess Plateau from 22 to 3.5 Ma. Earlier studies[9] place the Miocene loess deposits into the so-called Gansu Sys-tem. Our investigations show that the Gansu System contains inde…     

河南\

随机抽查统计了大气科学类18种核心期刊和15种省级刊物中的50期共计605篇论文,发现38%左右的关键词或摘要撰写存在欠规范的问题。为了提高学术论文的撰写质量,对大气科学类学术论文中关键词标引和摘要撰写存在的问题及解决方法进行了探讨。研究表明：关键词主要存在选用通用词、漏选关键词、标引深度不合适、排列顺序混乱等不足,摘要比较常见的问题则表现在基本要素残缺、嵌入不当信息、结果（结论）笼统空泛。该研究指出：关键词的选取以《汉语主题词表》中的叙词为主,可适当搭配新型交叉学科、新理论、新技术中出现的科技术语和专有名词,忌选通用词;应遵循“选准、选全”的原则,从题名、摘要、层次标题、结论乃至正文中提取能完整表达论文主题、具有检索价值的主题词,以4~5个为宜;其排序应遵循一定的逻辑规则。摘要应包含目的、方法、结果、结论四要素;避免使用第一人称、嵌入背景知识、自我见解和主观评价;结果（结论）部分要逻辑清晰、言之有物、定量直观,让读者能透过研究结果窥见论文的核心价值。该文还提出了提炼摘要四要素的四个步骤及大气科学类摘要的三种常见写作格式。

     

内蒙古和林格尔新区核心区地下空间适宜性评价

本文以内蒙古和林格尔新区核心区为研究范围,利用已有调查数据,基于MapGIS平台,从水文地质、工程地质、不良地质及地貌4个方面选取9个因子组成了地下空间适宜性评价指标体系,采用层次分析法对研究区地下空间适宜性进行了评价。评价结果显示：研究区浅层地下空间较适宜区占总面积99.71%;中层地下空间较适宜区占总面积95.03%;深层地下空间较适宜区分布于研究区东南部,占总面积26.95%,次适宜区大面积分布于研究区中西部区域,占总面积72.27%。研究结果表明：研究区地下空间资源综合适宜性等级基本以较适宜、次适宜为主,区内不同竖向层次与不同区位地下空间资源的适宜性存在差异,资源条件总体上呈现\     

融合多源海洋大地测量数据的南海海底地形多层感知机反演

本文融合SIO(Scripps Institution of Oceanography)发布的垂线偏差、重力异常和垂直重力梯度数据及NCEI(National Centers for Environmental Information)发布的船载测深数据, 利用多层感知机神经网络(Multi-Layer Perceptron, MLP)建立南海海域(108°E—121°E, 6°N—23°N)分辨率为1'×1'的海底地形模型(MLP_Depth).首先, 将642716个船载测深控制点的位置信息与周围4'×4'格网点处的地球重力信息(垂线偏差、重力异常、垂直重力梯度)作为输入数据, 将船载测深控制点处实测水深值作为输出数据, 训练MLP神经网络模型, 训练结束时决定系数R²为99%, 平均绝对误差MAE为39.33 m.然后, 将研究区域内1'×1'格网正中心点处的输入数据输入于MLP模型中, 可得格网正中心点处的预测海深值.最后, 根据预测海深值建立研究区域范围内分辨率为1'×1'的MLP_Depth模型.将MLP_Depth模型预测水深与160679个检核点处实测水深对比, 其差值的标准差STD(75.38 m)、平均绝对百分比误差MAPE(5.89%)与平均绝对误差MAE(42.91 m)皆优于GEBCO_2021模型、topo_23.1模型、ETOPO1模型与检核点实测水深差值的STD(108.88 m、113.41 m、229.67 m)、MAPE(6.11%、6.94%、18.37%)与MAE(47.33 m、52.24 m、130.08 m).同时, 为了研究不同区域内利用该方法建立的海底地形模型的精度, 本文在研究区域内分别建立了A、B区域的海底地形模型(MLP_Depth_A、MLP_Depth_B).经过验证得: MLP_Depth_A、MLP_Depth_B相比于MLP_Depth模型具有更高的精度, 更能反应海底地形的变化趋势.

     

鄂尔多斯盆地东部深部煤层气井压裂工艺及实践

鄂尔多斯盆地东部深部煤层气（埋深大于1 200 m）资源丰富,是下步煤层气勘探、开发的重点。与浅部煤层压裂相比,深部煤层压裂存在诸多挑战（地应力、闭合应力、破裂压力高,岩石力学参数复杂等）。通过对比现有煤层压裂工艺,以鄂尔多斯盆地东部深部煤层（埋深约2 000 m）为例,探索了采用油管+循环滑套+封隔器+喇叭口施工管柱和低浓度线性胶压裂液体系对深部煤层进行压裂作业,施工比较顺利,见气快,产气效果比较理想（产气量大于800 m³/d）。通过2口井的现场作业实践,证明了该工艺技术简单、易行、有效,为后续深部煤层气的勘探开发提供可借鉴的工艺技术。     

2017年江西汛期设区市城区暴雨回波特征分析

使用江西WebGIS雷达拼图和自动站雨量、雷电监测、强天气监测等数据,以及MICAPS常规天气图资料,对2017年3—7月江西汛期11个设区市26次城区暴雨过程的雷达回波特征进行分析。结果表明:2017年江西汛期共出现52日暴雨过程,其中江西11个设区市所在地城区出现26次暴雨。在这26次城区暴雨个例中,有23次伴随出现短时强降水,有3次降水比较均匀。暴雨维持时间长短不一,最长的有15 h,最短只有3 h,平均是10 h。有5次出现大风天气,21次没有大风出现。雷达回波特征主要有3种:块状(强单体、超级单体)、带状(飑线、回波带)、絮状(絮带、絮团)。这3种回波形态特征,出现率最高的是絮状回波,即比较宽、嵌有中等强度的对流单体絮带状回波带,强度40～55 dBz,出现16次,概率62%;其次是窄而长、紧密排列由强单体组成的飑线回波带,强度50～60 dBz,出现8次,概率30%;块状(强单体、超级单体)回波强度最强,中心强度达到60～70 dBz,出现2次,概率8%。