日本囊对虾Marsupenaeus japonicus)组织蛋白酶D基因克隆及表达分析

组织蛋白酶D是溶酶体天冬氨酸蛋白酶家族的主要成员,广泛参与动物机体细胞内蛋白质的降解过程,对维持细胞稳态和正常代谢具有重要的作用。为研究组织蛋白酶D在甲壳动物非特异性免疫和幼体发育过程中的作用,本研究采用RACE技术首次克隆得到日本囊对虾组织蛋白酶D基因cDNA序列,命名为MjCatD,其中开放阅读框长为1161 bp,编码386个氨基酸残基。序列分析和同源建模显示该基因编码的蛋白含有保守的N-糖基化位点、天冬氨酸蛋白酶签名序列、酶活化位点和非消化性组织蛋白酶D的特征序列,并且呈保守的双叶形结构。同源性比较和系统进化分析发现,MjCatD与斑节对虾、美洲螯龙虾和脊尾白虾相似性较高,并且与它们紧密聚为一支。实时荧光定量PCR结果显示,MjCatD基因在日本囊对虾多个组织中均有表达,其中肝胰腺中表达量最高。在白斑综合征病毒(white spot syndrome virus, WSSV)感染后3～24 h日本囊对虾肝胰腺中MjCatD的表达量逐渐下降,而在48 h急剧上调至最高表达量并且与对照组差异极显著(P<0.01)。此外,MjCatD基因在幼体发育不同阶段中也表现出明显的变化趋势。以上研究表明,MjCatD基因可能参与日本囊对虾先天免疫反应和幼体发育过程。     

基于海浪谱分解与重构的资料同化试验

针对有效波高资料提出一种海浪谱分解与重构的资料同化方案:利用历史时段内的有效波高观测资料和模式计算波高场,采用最优插值方法得到分析波高场;在WAVEWATCH-Ⅲ模式的波浪能量密度谱和有效波高分析值之间引入一个变异系数矩阵,描述模式的误差,以此为状态向量构建卡尔曼滤波系统,对分解过的海浪谱进行修正和重构,得到同化后的海浪谱初始场。利用美国阿拉斯加湾北部海域的7个浮标站进行同化和72 h预报试验,对连续1个月的预报结果进行统计表明:采用该同化方案后24 h预报结果的有效波高均方根误差比未同化的结果降低了0.13 m;同化方案对预报效果的影响可持续36 h左右,随着预报时效延长,同化的效果减弱。     

白令海夏季浮游植物的群落结构与时空变化

通过1999年和2010年夏季同期7月在白令海(169°E~166°W,50°N~67°N)获取的94份浮游植物样品分析,获得了近十年的始末两个时间节点的浮游植物群落结构与时空变化,探讨了浮游植物群落动态及其与环境因素的关联。研究结果显示,共鉴定浮游植物(>10μm)5门58属153种,分为3个生态类群。硅藻是浮游植物的主体,种类多丰度高,占总种类数目的66.7%,占总丰度的95.2%。鉴于样品属性和空间范围的不同,物种组成有细微差别,丰度有较大差异且空间分布明显不同,高丰度区受控于上层营养盐供给和表层环流系统。优势种从北方温带大洋性硅藻演变为广温广盐性与冷水性硅藻,1999年以西氏新细齿状藻为第一优势种,柔弱伪菱形藻次之;2010年以丹麦细柱藻为第一优势种,冷水性的诺登海链藻次之并在陆架和陆坡占优。浮游植物群落结构较为稳定,由深水群落和浅水群落组成。深水群落分布于太平洋西北部和白令海盆,种类组成以温带大洋性的西氏新细齿状藻、长海毛藻、大西洋角毛藻和广布性的菱形海线藻、扁面角毛藻、笔尖根管藻为主,丰度低,种间丰度分配均匀,优势种多元化,物种多样性高;浅水群落分布于白令海陆坡和陆架,主要由冷水性的诺登海链藻、叉尖角毛藻、聚生角毛藻和广布性的丹麦细柱藻、旋链角毛藻组成,丰度高,种间丰度分配不均匀,优势种突出,物种多样性低。白令海夏季浮游植物种类组成及丰度变化直接受控于表层环流、营养盐、春季冰缘线等环境因素。     

Numerical study on inter-tidal transports in coastal seas

Inter-tidal(subtidal) transport processes in coastal sea depend on the residual motion, turbulent dispersion and relevant sources/sinks. In Feng et al.(2008), an updated Lagrangian inter-tidal transport equation, as well as new concept of Lagrangian in- ter-tidal concentration(LIC), has been proposed for a general nonlinear shallow water system. In the present study, the LIC is nu- merically applied for the first time to passive tracers in idealized settings and salinity in the Bohai Sea, China. Circulation and tracer motion in the three idealized model seas with different topography or coastline, termed as ‘flat-bottom', ‘stairs' and ‘cape' case, re- spectively, are simulated. The dependence of the LIC on initial tidal phase suggests that the nonlinearities in the stairs and cape cases are stronger than that in the flat-bottom case. Therefore, the ‘flat-bottom' case still meets the convectively weakly nonlinear condi- tion. For the Bohai Sea, the simulation results show that most parts of it still meet the weakly nonlinear condition. However, the de- pendence of the LIS(Lagrangian inter-tidal salinity) on initial tidal phase is significant around the southern headland of the Liaodong Peninsula and near the mouth of the Yellow River. The nonlinearity in the former region is mainly related to the complicated coast- lines, and that in the latter region is due to the presence of the estuarine salinity front.     

A New Ootype of Dinosaur Egg (Faveoloolithidae: Duovallumoolithus shangdanensis oogen. et oosp. nov.) from the Late Cretaceous in the Shangdan Basin,Shaanxi Province,China

A new ootype collected from the Upper Cretaceous Lijiacun Formation in the Shangdan Basin, Shaanxi Province is described in this paper. Based on general external shape, size, eggshell thickness and honeycomb‐like eggshell microstructure, eggs are referable to the oofamily Faveoloolithidae. Compared with other members of Faveoloolithidae, specimens described in this paper show special characteristics: adjacent pores are usually separated by two eggshell units between which often develop interspaces; columnar eggshell units are relatively closely arranged in radial view. According to these characteristics, we erect a new oogenus and a new oospecies: Duovallumoolithus shangdanensis oogen. et oosp. nov. The new discovery expands the diversity of Faveoloolithidae.     

Development of efficiently coupled thermo-hydro-mechanical model to predict hydraulic fracture morphology in heavy oil reservoirs

The aim of the study involves examining the effect of heavy oil viscosity on fracture geometry in detail by establishing a heavy oil fracturing model and conventional fracturing model based on thermal–hydraulic–mechanical (THM) coupled theory, Walther viscosity model, and K–D–R temperature model. We consider viscosity and density within the heavy oil fracturing model as functions of pressure and temperature while that as constants within the conventional fracturing model. A heavy oil production well is set as an example to analyze the differences between the two models to account for the thermo-poro-elastic effect. The results show that temperature exhibits the most significant influence on the heavy oil viscosity while the influence of pressure is the least. In addition, a cooling area with a width of 0–1 m and varied length is generated near the fracture. The heavy oil viscosity increases sharply in this area, thereby indicating an area of viscosity increment. The heavy oil viscosity increases faster and is closer to wellbore, and a high viscosity increment reduces the mobility of the heavy oil and prevents the fracturing fluid from entering into the reservoir. The special viscosity distribution results in significant differences in pore pressure, oil saturation, and changing trends between these two models. In the heavy oil reservoir fracturing model, the thermal effect completely exceeds the influence of pore elasticity, and the values of the fracture length, width, and static pressure exceed those calculated in the conventional fracturing model. Thus, a comparison of the measured values indicates that the results obtained by considering viscosity as a function of temperature and pressure are more accurate. Therefore, the results of this study are expected to provide good guidelines for the design of heavy oil fracturing.     

合肥盆地安参1井前侏罗纪基底地层的孢粉组合特征与时代归属

大别山北缘的安参1井是合肥盆地最深的一口参数井,钻遇前侏罗纪基底地层达千米以上,为一套不含煤层(线)或灰岩以泥岩为主的地层,与周缘华北陆块和大别造山带等构造单元的地层对比困难。因缺少宏观化石记录和明确的定年依据,其时代归属一直存在争议,制约了对这一套特殊地层所反映的地质信息的进一步挖掘。为进一步明确该套特殊地层的地质时代,本项研究对安参1井4160~5152m井段大量岩心和岩屑样品进行了微古化石分析,在其中5块样品中发现了保存良好的孢粉化石647粒,共计23属51种及部分未定种。根据对孢粉化石的类型、属种和含量的分析,将其自下而上划分为两个孢粉化石组合:Ⅰ.Densosporites reynoldburgensisLaevigatosporites perminutus;Ⅱ.Triquitrites-Macrotorispora media。孢粉组合Ⅰ中蕨类植物孢子以平均含量87.67%占绝对优势,代表分子有Laevigatosporites perminutus,Lycospora rotunda,Patellisporites meishanesis等,裸子植物花粉均为单囊粉属Florinites;孢粉组合Ⅱ中,蕨类植物孢子平均含量降低至53.78%,其中蕨类孢子Lycospora rotunda消失,Leiotriletes adnatus,Triquitrites,Crassipora等含量下降,并有特征分子大一头沉孢属Macrotorispora出现,裸子植物花粉含量和丰富度明显增加,以柯达粉属Cordaitina为主。经过与华北其他地区孢粉组合特征的对比分析,上述两个孢粉组合分别与华北陆块晚古生代二叠系太原组和上石盒子组具有明确的相似性,与早期和中晚期华夏植物群面貌相一致,反映了裸子植物逐渐繁盛、属种越来越丰富的过程,孢粉组合Ⅰ和Ⅱ的地质时代分别为晚石炭世晚期至早二叠世早期和晚二叠世,为安参1井前侏罗纪基底存在石炭—二叠系提供了直接证据,也为合肥盆地沉积环境和古气候分析提供了新资料。     

Paleo-environment and reservoir evolution of the Middle Ordovician Kelimoli Formation in the northern Tianhuan area,Ordos Basin,China: implications for high-quality reservoir development

The Middle Ordovician Kelimoli Formation carbonate reservoirs in the northern Tianhuan area, Ordos Basin, China are main exploration targets. Subsurface core samples, logging, drilling and production data from the area were used to characterise the carbonate reservoir and to construct a genetic model for exploration. The sedimentary facies identified include trough–continental rise, upper–lower slope, platform margin reef-shoal, open platform and evaporation-restricted platform. The slope and platform margin facies are potential zones for high-quality reservoirs. Porosity in the study area comprises intergrain, intercrystal, intragrain and intracrystal pores, fractures and vughs. The Sr/Ba (0.40–4.87) and V/(V + Ni) (0.64–0.97) ratios indicate deposition in a brackish water-dominated environment under reducing conditions, associated with sea-level fluctuations during the deposition of the Kelimoli Formation. The Sr/Ba, V/(V + Ni), ⁸⁷Sr/⁸⁶Sr ratios, δ¹⁸O values and crystal texture of dolomite samples suggest that meteoric water was involved in the diagenetic fluid in the near-surface depositional environment. Isotopes (δ¹³C and δ¹⁸O) and trace elements (Fe and Mn) allowed the identification of seepage-reflux and mixed-water dolomitisation. The crystal textures of the samples consist of micritic, bioclastic and reefal limestones, and dolomite with gypsum, which were easily dissolved during the early diagenetic stage. The epidiagenetic stage was the key period for the development of high-quality reservoirs because of large-scale bedding-parallel karstification from meteoric water. The reservoir zones, dominated by partially filled and unfilled vughs, and fractures, are favourable exploration targets in the northern Tianhuan area.     

Progress in the Application of Ground Gravity Observation Data in Earthquake Prediction

The mechanism of earthquake inoculation and the process of earthquake occurrence are very complicated. Additionally, earthquakes do not happen very often, and we lack enough cognition to the earth’s interior structure, activity regularity and other key elements. As a result, research progress about the theory of earthquake precursors has been greatly restricted. Ground gravity observation has become one of the main ways to study earthquake precursor information in many countries and regions. This paper briefly summarized the surface gravity observation technology and observation network in China: the surface gravity measurement instrument developed from Huygens physical pendulum in seventeenth Century to today’s high-precision absolute gravimeter, and its accuracy reached to ±1×10^-8 m/s². China has successively established the National Gravity Network, Digital Earthquake Observation Network of China,the Crustal Movement Observation Network of China Ⅰ and the Crustal Movement Observation Network of China, to provide a public platform for monitoring non tidal gravity change, seismic gravity and tectonic movement. The use of specific examples illustrated the role of gravity observation data in earthquake prediction. The gravity observation data of ground gravity can be used to capture the information of gravity change in the process of strong earthquake inoculation, and to provide an important basis for the long-term prediction of strong earthquakes. The temporal and spatial variation characteristics of the regional gravity field and its relation to strong earthquakes were analyzed: Before the earthquake whose magnitude is higher than M_S 5, generally there will be a large amplitude and range of gravity anomaly zones. Strong earthquakes occur mainly in areas where the gravity field changes violently. The dynamic change images of gravity field can clearly reflect the precursory information of large earthquakes during the inoculation and occurrence. Finally, the existing problems of surface gravity technology in earthquake precursor observation were put forward and the use of gravity measurement data in earthquake prediction research was prospected.