深海细菌Pseudomonas sp. IOFA1中甲醛歧化酶FM11的纯化及性质研究

从印度洋深海沉积物中分离出一株能够高效降解甲醛的假单胞菌(Pseudomonas sp.IOFA1),该菌株可在40 min内完全降解100 mg/cm~3的甲醛.通过阴离子交换柱层析对菌株破碎液中的粗酶液进行纯化,得到一条具有甲醛降解活性的单一蛋白条带.经MALDI-TOF/TOF质谱鉴定,该蛋白为甲醛歧化酶蛋白(FM11).序列分析表明FM11与其它物种已知醛脱氢酶的氨基酸序列最高相似性为65%;结构域分析表明FM11含有2个保守的锌结合位点和一个NAD结合位点.酶学性质分析结果显示,FM11的最适反应温度为40℃,在25~50℃范围内能够保留95%以上的酶活力,尤其是在高于70℃的高温环境下仍保有约50%的活力,表现出广泛的温度适应性.FM11具有非常广泛的最适反应pH值范围(pH值为5~9),且在pH值4和pH值10时仍分别保留50%和80%左右的酶活力,表现出广泛的pH适应性.在pH值为5~7的条件下孵育1 h后,FM11仍保有97%以上的酶活,且在pH值为7~10条件下孵育1 h后,仍保留90%以上的酶活,表现出极好的酸性和碱性稳定性.Ca~(2+)、Mg~(2+)在低浓度时(0.5 mmol/dm~3)对FM11有较明显的激活作用.这些研究为我们进一步开发Pseudomonas sp.IOFA1甲醛歧化酶在降解甲醛方面的用途奠定了基础.     

Petrogenesis and Tectonic Significance of the Three‐Period Porphyries from the Daruoluolong Cu (Au) Deposit,Tibet, China

正Objective The Daruoluolong deposit is the first high-grade Cu(Au)deposit discovered in the middle section of the Bangonghu-Nujiang(herein after referred to as Ban-Nu)metallogenic belt,which has not been documented about previously.This deposit is located in Shuanghu County of northern Tibet,and its geotectonic position belongs to the     

桂北地区罗地1井钻探施工实践

页岩具有较强的水敏性,在页岩气勘查钻进施工中,井内易发生坍塌、剥落造成超径或缩径等井壁失稳现象。罗地1井采用金刚石绳索取心工艺进行钻进,完井井深1501.06 m,岩心采取率、井斜等各项指标均符合地质设计要求。本文主要从罗地1井的钻井设备、井身结构、施工难点等方面进行总结,可为今后页岩气调查井的施工提供参考。     

川东长兴组台缘礁滩相储层纵向非均质性特征及形成机制——以川东宣汉盘龙洞长兴组剖面为例

近几年,随着四川盆地海相碳酸盐岩储层勘探的不断加强,碳酸盐岩储层强的非均质性已成为制约礁滩气藏勘探开发的现实问题。以川东宣汉盘龙洞长兴组典型台缘礁滩剖面为研究对象,通过对典型台缘礁滩野外剖面的精细观察,结合镜下薄片及岩矿资料分析,重点探讨了盘龙洞礁滩储层纵向非均质性特征及成因。剖面上礁滩体垂向上存在很强的旋回性和储层非均质性,其储集岩分布、储层厚度、白云岩化程度、溶蚀作用等受海平面周期性的升降变化影响而呈现周期性变化。礁滩储层非均质性受高能相带、海平面升降变化和建设性成岩作用的共同控制,高能相带控制储层原生孔隙的发育,决定了储层的沉积非均质性,白云岩化和溶蚀作用有利于礁滩体储层的形成,大气淡水淋滤改造对于优化储层具有关键作用,海平面升降变化决定了礁滩体旋回性发育,而建设性成岩作用的选择性改造深化了储层非均质性。     

贵州遵义深溪大型锰矿床主要地质特征

贵州遵义地区是我国二叠纪锰矿的重要分布区之一,深溪锰矿是遵义地区继铜锣井锰矿发现的第二个大型隐伏锰矿床。本文结合深溪锰矿找矿实践,详细总结了深溪锰矿床地层、构造、矿石等特征,并对锰矿的矿床成因进行了探讨。研究表明,深溪锰矿严格受地层层位控制,呈层状、似层状产于二叠纪茅口组第三段浅灰至灰黑色含锰岩系中,矿石矿物主要为菱锰矿,含少量黄铜矿、重晶石、毒砂等矿物,矿石为块状、斑杂状、角砾状构造,自然类型为碳酸锰矿石,工业类型为贫锰矿、富锰矿。分析认为深溪锰矿为深部的气液成矿物质喷溢至地堑盆地中沉积成矿,为"内生外成"的"古天然气渗漏沉积型锰矿床"。     

Residual-state creep of clastic soil in a reactivated slow-moving landslide in the Three Gorges Reservoir Region,China

We study the creep properties of clastic soil in residual state. The intact samples are taken from a reactivated slow-moving landslide in the Three Gorges Reservoir Region, China. Firstly, the patterns of the landslide movement are analysed based on recent monitoring data, which indicate that the soil within the shear zone is undergoing two deformation processes: a creep phase, characterised by different creep rates, and a dormant phase. We then study the creep behaviour of the soil samples through a series of ring shear creep tests under various shear stress conditions. The creep response depends strongly on the ratio of the shear stress to the residual strength, and the normal effective stress, whereas the creep rate decreases due to strength regain. The long-term strength of the clastic soil is close to the residual strength. Therefore, the residual strength obtained from conventional shear test, which is less time consuming than creep test, can be used in long-term stability analyses of creeping landslides.     

Numerical simulation of lifetime and time-dependent damage for granite by continuous and discontinuous approaches

In this study, the time-dependent damage process of granite is investigated utilizing two numerical simulation schemes based on continuous method and discontinuous method. Numerical creep tests are carried out with both simulation schemes and mechanical responses and fracture patterns of rock specimens are analyzed. The calibrated numerical models can successfully reflect the typical creep stages observed in the laboratory. The predicted lifetime is in accordance with the laboratory test data. Comparisons are made between the two simulation schemes. It is found that both schemes have unique features that can promote a genuine reflection of the time-dependent damage process of the brittle rocks.     

Carbonate formation mechanism in paleosol sediments of the Bohai Sea coastal zone,China

The Bohai Sea coastal zone of China consists of the Pacific Ocean to the east and Eurasia to the west; hence, this region is influenced by both the ocean and continental landmasses. The carbonate formation mechanism of eolian sediment within this area is poorly understood. The loess-paleosol sediments of the Miaodao stratigraphical section (MDS) contain a record of carbonate changes in this region during the last interglacial period. New insights into regional carbonate formation mechanisms since the last interglacial period were obtained by analyzing the ages of various sedimentary facies in combination with proxy paleoclimatic indices (including average grain size, standard deviation, CaCO₃ content, and clay minerals), as well as via foraminiferal analysis. The results led to three principal findings: (1) The carbonate content change in the MDS was neither controlled by grain size nor affected by minerals. The carbonate change controlled by precipitate leaching in the Loess Plateau region cannot explain the eolian sediments within the Bohai Sea coastal zone. (2) Various subsections contain obvious carbonate content changes caused by foraminiferal deposits atop the eolian sediments, which were deposited by storm surges during a high sea-level period. This increased the carbonate content in the eolian sediments and restricted the carbonate content in the Bohai Sea coastal zone. (3) Newly detected foraminifera of the last interglacial period on the eolian sediment of the MDS were the main source of carbonate content, providing a new understanding of the carbonate formation mechanism in eolian sediments different from that of the Loess Plateau.     

准噶尔盆地东北部侏罗系砂岩型铀矿成矿环境与找矿方向探讨

文章从蚀源区铀源条件、中新生代构造演化条件、侏罗系沉积建造特征及后生蚀变特征等角度分析了准噶尔盆地东北部砂岩型铀矿的区域成矿条件,认为卡拉麦里山的富铀花岗岩体为该区砂岩型铀成矿提供了丰富的铀源,中侏罗统含煤碎屑岩建造为砂岩型铀成矿提供了良好的容矿空间,侏罗纪以来多期次不整合面的发育为含铀含氧水的渗入改造提供了通道及时间,为大规模层间氧化带的发育提供了物质基础。研究结果显示,卡拉麦里山北坡为最有利的砂岩型铀矿找矿部位,其主要找矿目标层位为中侏罗统头屯河组,找矿类型为层间氧化带型。     

高效液相色谱-电感耦合等离子体质谱联用检测土壤中的无机硒形态

土壤样品中亚硒酸盐Se(Ⅳ)和硒酸盐Se(Ⅵ)的形态分析中,提取剂的选择和检测方法是技术的关键。以往的提取剂容易导致硒形态发生转变或无法同时提取Se(Ⅳ)和Se(Ⅵ),常用的氢化物发生原子荧光光谱法无法直接测定Se(Ⅵ),而是通过差减法得出Se(Ⅵ)含量。本文对比了不同提取剂的提取能力,确定使用0. 1 mol/L氢氧化钠溶液作为提取剂,在55℃超声萃取土壤样品30 min,提取液经高效液相色谱分离,电感耦合等离子体质谱检测,建立了土壤中Se(Ⅳ)和Se(Ⅵ)的形态分析方法。采用Hamilton PRP X-100色谱柱,以6 mmol/L柠檬酸为流动相,pH=5. 5,在8 min内可完全分离Se(Ⅳ)和Se(Ⅵ),两者的检出限分别为0. 15μg/L、0. 16μg/L,线性相关系数(r~2)均大于0. 999。以土壤为基体进行加标回收试验,Se(Ⅳ)和Se(Ⅵ)的回收率在84. 2%～95. 8%之间,相对标准偏差为1. 4%～5. 3%(n=6)。该方法简单快速,具有良好的精密度和准确度,适用于土壤中无机硒的形态分析。