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151.
长牡蛎碱性磷酸酶的分离纯化及部分性质研究 总被引:5,自引:1,他引:5
本文以长牡蛎为酶源提取材料,用正丁醇抽提,硫酸铵分级分离,DEAE-52和Sephadex G-100柱层析,得到一定纯度的碱性磷酸酶,提纯倍数为61.96,比活力达1.326U/mg.该酶的最适pH值9.6,最适温度38℃,初速度6.8μmol/(dm~3·min),米氏常数Km值为1.30mmol/dm~3.实验结果表明,Mg~(2+)对该酶有明显的激活作用;Zn~(2+)、Cu~(2+)、Hg~(2+)对该酶有明显的抑制作用. 相似文献
152.
Tiago M. Alves Vasilios Lykousis Dimitris Sakellariou Stamatina Alexandri Paraskevi Nomikou 《Geo-Marine Letters》2007,27(1):41-61
Bathymetric, 9.5-kHz long-range sidescan sonar (OKEAN), seismic reflection and sediment-core data are used in the analysis
of two tectonic troughs south of Crete, Eastern Mediterranean Sea. Here, up to 1.2 s two-way travel time (TWTT) of strata
have accumulated since the Middle Miocene in association with extension in the South Aegean region. The study area comprises
>100-km- long by >25-km-wide basins filled by sediments subdivided into two seismic units: (1) an upper Unit 1 deposited in
sub-basins which follow the present-day configuration of the southern Cretan margin; (2) a basal Unit 2, more than 500 ms
(TWTT) thick, accumulated in deeper half-graben/grabens distinct from the present-day depocentres. Both units overlap a locally
stratified Unit 3 comprising the pre-Neogene core complex of Crete and Gavdos. In this work, the interpreted seismic units
are correlated with the onshore stratigraphy, demonstrating that denudation processes occurring on Crete and Gavdos in response
to major tectonic events have been responsible for high sedimentation rates along the proximal southern Cretan margin. Consequently,
topographically confined sedimentary units have been deposited south of Crete in the last 12 Ma, including turbidites and
other mass-flow deposits fed by evolving transverse and axial channel systems. Surface processes controlling facies distribution
include the direct inflow of sediment from alluvial-fan systems and incising mountain rivers onto the Cretan slope, where
significant sediment instability processes occur at present. In this setting, seismic profiles reveal eight different types
of stratigraphic contacts on basin-margin highs, and basinal areas show evidence of halokinesis and/or fluid escape. The acquired
data also show that significant changes to the margin’s configuration occurred in association with the post-Alpine tectonic
and eustatic episodes affecting the Eastern Mediterranean. 相似文献
153.
154.
The present paper deals with the ultrastructural changes of oocyte in the process of maturing in pen shell Atrinapectinata Linne. When it develops from the antecedent growth period to the accelerating growth period the annulate lamellae emerge, the RER better develop, the yolk granules with single and double layer membrane and are visible. In the well-developed mitochondria there is yolk stored. The Golgi vacuoles are brewing yolk granules. At the mature period the annulate lamellae and the Golgi bodies disappear, and there are only a few RER and mitochondria with poor cristae in the cytoplasm. All these might be well used for judging the maturity of the ootids in the process of the artificial propagation. 相似文献
155.
156.
Noriaki Kimura 《Journal of Oceanography》2007,63(4):685-694
This paper examines the mechanism controlling the short time-scale variation of sea ice cover over the Southern Ocean. Sea
ice concentration and ice velocity datasets derived from images of the Defense Meteorological Satellite Program (DMSP) Special
Sensor Microwave Imager (SSM/I) are employed to reveal this mechanism. The contribution of both dynamic and thermodynamic
processes to the change in ice edge location is examined by comparing the meridional velocity of ice edge displacement and
sea ice drift. In the winter expansion phase, the thermodynamic process of new ice production off the ice edge plays an important
role in daily advances of ice cover, whereas daily retreats are mostly due to southward ice drift. On the other hand, both
advance and retreat of ice edges in the spring contraction phase are mostly caused by the dynamic process of the ice drift.
Based on the above mechanism and the linear relation between the degree of ice production at the ice edge and northward wind
speed, the seasonal advance of ice cover can be roughly reproduced using the meridional velocity of ice drift at the ice edge. 相似文献
157.
Two distinct series of slumps deform the upper part of the sedimentary sequence along the continental margin of the Levant.
One series is found along the base of the continental slope, where it overlies the disrupted eastern edge of the Messinian
evaporites. The second series of slumps transects the continental margin from the shelf break to the Levant Basin. It seemed
that the two series were triggered by two unrelated, though contemporaneous, processes. The shore-parallel slumps were initiated
by basinwards flow of the Messinian salt, that carried along the overlying Plio-Quaternary sediments. Seawater that percolated
along the detachment faults dissolved the underlying salt to form distinctly disrupted structures. The slope-normal slumps
are located on top of large canyons that cut into the pre-Messinian sedimentary rocks. A layer of salt is found in the canyons,
and the Plio-Quaternary sediments were deposited on that layer. The slumps are bounded by large, NW-trending faults where
post-Messinian faulted offset was measured. We presume that the flow of the salt in the canyons also drives the slope-normal
slumps. Thus thin-skinned halokynetic processes generated the composite post-Tortonian structural patterns of the Levant margin.
The Phoenician Structures are a prime example of the collapse of a distal continental margin due to the dissolution of a massive
salt layer. 相似文献
158.
对虾一种无包涵体杆状病毒病原的PCR检测 总被引:4,自引:0,他引:4
为建立对虾无包涵体杆状病毒的快速诊断技术,应用PCR技术分别对暴发性流行病发生前、流行中和发病后的对虾样品进行了检测.根据已克隆的对虾杆状病毒部分基因组序列而设计的引物能特异性地扩增出靶DNA片段,最低可以检测1pg的病素DNA.由典型发病症状对虾的胃、腮、肝胰腺、中肠、游泳足、肌内和心脏等器官和组织中成功地检测到病毒.不同组织和器官经扩增得到的信号强弱不同:病虾的胃扩增得到的信号最强,中肠、肌肉、心脏和游泳足次之,腮和肝胰腺信号最弱,说明病毒在不同组织和器官中数量及感染程度不同.在对虾发病前及发病后,用二次PCR还能检测表面不发病呈隐性感染状态的对虾携带的病毒;而对野生健康虾的检测结果为阴性.研究表明,PCR是检测对虾暴发性流行病快速、灵敏而准确的方法,对病毒病的早期诊断、防治和高健康对虾品种的选育具有指导意义. 相似文献
159.
160.
Seasonal evolution of surface mixed layer in the Northern Arabian Sea (NAS) between 17° N–20.5° N and 59° E-69° E was observed
by using Argo float daily data for about 9 months, from April 2002 through December 2002. Results showed that during April
- May mixed layer shoaled due to light winds, clear sky and intense solar insolation. Sea surface temperature (SST) rose by
2.3 °C and ocean gained an average of 99.8 Wm−2. Mixed layer reached maximum depth of about 71 m during June - September owing to strong winds and cloudy skies. Ocean gained
abnormally low ∼18 Wm−2 and SST dropped by 3.4 °C. During the inter monsoon period, October, mixed layer shoaled and maintained a depth of 20 to
30 m. November - December was accompanied by moderate winds, dropping of SST by 1.5 °C and ocean lost an average of 52.5 Wm−2. Mixed layer deepened gradually reaching a maximum of 62 m in December. Analysis of surface fluxes and winds suggested that
winds and fluxes are the dominating factors causing deepening of mixed layer during summer and winter monsoon periods respectively.
Relatively high correlation between MLD, net heat flux and wind speed revealed that short term variability of MLD coincided
well with short term variability of surface forcing. 相似文献