Cloning and expression analysis of the chloroplast fructose- 1,6-bisphosphatase gene from Pyropia haitanensis

Fructose-1,6-bisphosphatase(FBPase) is one of the key enzymes in Calvin circle and starch biosynthesis. In this study, the full-length of cpFBPase gene from Pyropia haitanensis was cloned by using rapid amplification of cDNA ends(RACE) technology. The nucleotide sequence of PhcpFBPase consists of 1 400 bp, including a 5′ untranslated region(UTR) of 92 bp, a 3′?UTR of 69 bp, and an open reading frame(ORF) of 1 236 bp, which can be translated into a 412-amino-acid putative peptides with a molecular weight of 44.3 kDa and a theoretical pI of 5.23. Multiple sequence alignment indicated that the protein belonged to the chloroplast FBPase enzyme. Phylogenetic analysis showed that the protein assembled with the cpFBPase of a thermal tolerant unicellular red micro-algae Galdieria sulphuraria. Expression patterns analyzed by qRT-PCR revealed that the expression of PhcpFBPase gene in the thallus phage was 7-fold higher than in the conchocelis phage, which suggested the different mechanisms of inorganic carbon utilization among the different life phages of P. haitanensis. And the different response modes of PhcpFBPase mRNA levels to high temperature and desiccation stress indicated that PhcpFBPase played an important role in responsing to abiotic stress.     

东太平洋海隆深海热液区沉积物古菌多样性分析

采用PCR-RFLP方法对东太平洋海隆深海热液区3个站位沉积物中的古菌多样性进行了初步研究.结果显示,从古菌16S rRNA基因文库中随机挑取的296个阳性克隆分属奇古菌门（Thaumarchaeota,47.64%）、广古菌门（Euryarchaeota,44.93%）、泉古菌门（Crenarchaeota,6.77%）和未分类古菌（0.68%）,其中优势菌群为奇古菌门的亚硝化侏儒菌属（Nitrosopumilus,35.47%）和广古菌门的热原体纲（Thermoplasmata,27.03%）,DHVE3、DHVE5、DHVE6、MBGB和MBGE类群在沉积物样品中也均有发现.另外,3个站位沉积物中古菌类群组成存在差异,S5-TVG1站位样品文库的97个古菌克隆分属奇古菌门（49.48%）、广古菌门（49.48%）和泉古菌门（1.03%）,S14-TVG10站位样品文库的103个古菌克隆由奇古菌门（84.47%）和广古菌门（15.53%）组成,S16-TVG12站位样品文库的96个古菌克隆包括广古菌门（71.88%）、泉古菌门（19.79%）、奇古菌门（6.25%）和未分类古菌（2.08%）.研究结果表明,东太平洋海隆深海热液区沉积物中古菌多样性丰富,存在着许多新的古菌菌群;不同站位古菌菌群结构以及多样性存在差异,这与其所处环境的热液活动密切相关.     

中国区域滨海湿地固碳能力研究现状与提升

对我国滨海湿地的固碳能力进行了评估,同时,对其固碳能力的遥感监测和评估方法进行了总结和分析,最后提出了滨海湿地固碳能力的提升技术。全球看来,我国滨海湿地的固碳能力总体上不高。国内看来,在北方泥炭地和植被覆盖率高,生长茂盛的区域,尤其是有红树林生长的区域固碳能力相对较高。滨海湿地主要分布在潮间带,人为监测困难,卫星遥感的出现为滨海湿地的监测带来很大方便,遥感监测已成为评估湿地固碳能力的一种高时效方法。滨海湿地固碳蕴藏着巨大潜力,提升滨海湿地的固碳能力可以大大降低全球CO2含量,对于改善全球温室效应有不可替代的作用。     

New Discovery of Oligocene Gastropod Opercula from the Yehucheng Formation in Northwestern China

Fourteen species of nonmarine gastropod opercula are found to be in high abundance in the Oligocene upper member of the Yehucheng Formation,Lanzhou Basin,northwest China.They are attributed to two families,namely Bithyniidae and Assimineidae,and four genera,namely Pseudemmericia,Bithynia,Mirolaminatus and Assiminea.Among them,three species are new,and they are Bithynia paramonolithic sp.nov.,B.obliquus sp.nov.,and B.disregularis sp.nov.Some of these species were first discovered in Paleogene strata of the vast Northwest China and can be compared with the opercula recovered from the Paleogene deposits in East and South China.It is inferred from the paleontology and sediment associations that the climate was slightly humid and the fauna probably inhabited the fresh or brackish water of a shallow lake in the Lanzhou Basin during the late early Oligocene period.     

构造地理要素趋势面的尺度转换普适性方法探讨

当前的遥感科学面临着遥感数据获取能力与数据应用能力之间突出的供需矛盾。尺度问题作为遥感科学中的关键问题,既限制了遥感作为一门科学向系统性、普适性的发展,又限制了遥感应用能力的发展。本文对定量遥感中的尺度问题进行了梳理,包括:遥感与传统站点观测之间的不一致、不同尺度遥感产品之间的不一致、机理模型的尺度适用问题,以及遥感产品与用户需求时空尺度间的不一致。对遥感中的尺度转换方法展开了讨论,总结了尺度转换的关键问题在于原数据信息量不足时引入额外信息和保留关键信息两方面。提出了构造地理要素趋势面的基本构想,搭建了一个具有普适性的尺度转换方法框架。核心内容是充分利用地表环境要素时间、空间上的信息作为先验知识,通过关联遥感观测新信息和先验趋势面生成指定时空尺度的地表要素产品。     

A Review of Aerosol Optical Properties and Radiative Effects

Atmospheric aerosols influence the earth's radiative balance directly through scattering and absorbing solar radiation, and indirectly through affecting cloud properties. An understanding of aerosol optical properties is fundamental to studies of aerosol effects on climate. Although many such studies have been undertaken, large uncertainties in describing aerosol optical characteristics remain, especially regarding the absorption properties of different aerosols. Aerosol radiative effects are considered as either positive or negative perturbations to the radiation balance, and they include direct, indirect (albedo effect and cloud lifetime effect), and semi-direct effects. The total direct effect of anthropogenic aerosols is negative (cooling), although some components may contribute a positive effect (warming). Both the albedo effect and cloud lifetime effect cool the atmosphere by increasing cloud optical depth and cloud cover, respectively. Absorbing aerosols, such as carbonaceous aerosols and dust, exert a positive forcing at the top of atmosphere and a negative forcing at the surface, and they can directly warm the atmosphere. Internally mixed black carbon aerosols produce a stronger warming effect than externally mixed black carbon particles do. The semi-direct effect of absorbing aerosols could amplify this warming effect. Based on observational (ground-and satellite-based) and simulation studies, this paper reviews current progress in research regarding the optical properties and radiative effects of aerosols and also discusses several important issues to be addressed in future studies.     

基于北京二号影像辅助nDSM的建筑物自动提取

针对建筑物提取方法缺乏泛化性的问题,本文提出了将nDSM、北京二号影像、NDVI、BAI的七通道图像相结合作为数据源的提取方法。采用随机森林、梯度提升机、支持向量机、BP神经网络分类器对建筑物进行提取获取最佳分类器模型,并运用二值化与开闭运算,以建筑物面积与最小外接矩形面积的比值为阈值,对建筑物分别进行最小外接矩形、DP算法拟合,优化建筑物提取结果。试验结果表明,梯度提升机(GBDT)较其他分类模型在不同场景下综合效果较好,F-score精度更高。     

滑坡灾害监测的圆弧合成孔径雷达大气相位校正方法

地基圆弧合成孔径雷达通过形变值测量实现滑坡预警,是一种重要的滑坡灾害监测遥感手段。大气相位校正影响形变值测量精度,是长时间稳定监测的关键技术。本文提出一种基于网格划分的两阶段大气相位校正方法。该方法通过特征提取及分类获得永久散射点,基于此实现监测点的自动筛选;利用网格估计大气相位,有效降低运算量,提高了计算效率;结合空间滤波和时间序列滤波,保证了大气相位估计的准确性。实测数据处理结果表明了该文所提方法在大气相位校正方面的有效性。     

高海拔隧道涌突水预测分析——以四川小卡子隧道为例

小卡子隧道是连接九龙县城和石棉县城的一条便利通道,它是连接S215和S211的绿色保障,九龙和石棉之间是海拔高度4 000 m以上,九龙县城周边为高原宽谷丘陵地区,地势起伏较大。九龙至周边沿线不良地质和特殊褶皱构造发育,其中冰雪害几乎每年冬春都发生,且影响线路较长,其它地质灾害也时常暴发,致使交通事故频繁发生,成为制约九龙县发展,因而急需要对线路进行改造,拟建连接九龙县至石棉县小卡子隧道即为公路改建工程。     

基于τ-p变换的地震道重构

基于τ-p变换原理,研究一种基于τ-p变换的地震数据重构方法,运用该方法对待重构道用零道替代,通过τ-p正反变换及一定的迭代处理实现地震道数据重构。理论模型与实际资料应用表明,基于τ-p变换的地震道重构方法效果明显,重构数据稳健。