Anaerobic Metabolism in Tidal Freshwater Wetlands: II. Effects of Plant Removal on Archaeal Microbial Communities

The interaction of plant and microbial communities are known to influence the dynamics of methane emission in wetlands. Plant manipulations were conducted in an organic rich (JB-organic) and a mineral rich (JB-mineral) site in a tidal freshwater wetland to determine if plant removal impacted archaeal populations. In concert, a suite of process-based measurements also determined the effects of plant removal on rates of methanogenesis and Fe-reduction. The microbial populations were analyzed with clone libraries of the SSU ribosomal RNA (rRNA) gene from selected plots, and terminal restriction length polymorphism (tRFLP) of the SSU rRNA and the methyl-coenzyme M reductase (mcrA) gene. Overall, methanogenesis dominated anaerobic carbon mineralization at both sites during the most active growing season. A total of 114 SSU rRNA clones from four different plots revealed a diversity of Euryarchaeota including representatives of the Methanomicrobiales, Methanosarcinales and Thermoplasmatales. The clone libraries were dominated by the Thaumarchaeota, accounting for 65 % of clones, although their diversity was low. A total of 112 tRFLP profiles were generated from 56 samples from 25 subplots; the patterns for both SSU rRNA and mcrA showed little variation between sites, either with plant treatment or with the growing season. Overall these results suggest that wetland soil archaeal populations were resilient to changes in the associated surface plant communities. The work also revealed the presence of novel, mesophilic Thaumarchaeota of unknown metabolic function.     

Geoarchaeological Investigations of Midden‐Formation Processes in the Early to Late Ceramic Neolithic Levels at Çatalhöyük,Turkey ca. 8550–8370 cal BP

Anthropogenic midden deposits are remarkably well preserved at the Neolithic settlement of Çatalhöyük and provide significant archaeological information on the types and nature of activities occurring at the site. To decipher their complex stratigraphy and to investigate formation processes, a combination of geoarchaeological techniques was used. Deposits were investigated from the early ceramic to late Neolithic levels, targeting continuous sequences to examine high resolution and broader scale changes in deposition. Thin‐section micromorphology combined with targeted phytolith and geochemical analyses indicates they are composed of a diverse range of ashes and other charred and siliceous plant materials, with inputs of decayed plants and organic matter, fecal waste, and sedimentary aggregates, each with diverse depositional pathways. Activities identified include in situ burning, with a range of different fuel types that may be associated with different activities. The complexity and heterogeneity of the midden deposits, and thus the necessity of employing an integrated microstratigraphic approach is demonstrated, as a prerequisite for cultural and palaeoenvironmental reconstructions.     

Bulk rock composition and geochemistry of olivine-hosted melt inclusions in the Grey Porri Tuff and selected lavas of the Monte dei Porri volcano, Salina, Aeolian Islands, southern Italy

The Aeolian Islands are an arcuate chain of submarine seamounts and volcanic islands, lying just north of Sicily in southern Italy. The second largest of the islands, Salina, exhibits a wide range of compositional variation in its erupted products, from basaltic lavas to rhyolitic pumice. The Monte dei Porri eruptions occurred between 60 ka and 30 ka, following a period of approximately 60,000 years of repose. The bulk rock composition of the Monte dei Porri products range from basaltic-andesite scoria to andesitic pumice in the Grey Porri Tuff (GPT), with the Monte dei Porri lavas having basaltic-andesite compositions. The typical mineral assemblage of the GPT is calcic plagioclase, clinopyroxene (augite), olivine (Fo_72?84) and orthopyroxene (enstatite) ± amphibole and Ti-Fe oxides. The lava units show a similar mineral assemblage, but contain lower Fo olivines (Fo_57?78). The lava units also contain numerous glomerocrysts, including an unusual variety that contains quartz, K-feldspar and mica. Melt inclusions (MI) are ubiquitous in all mineral phases from all units of the Monte dei Porri eruptions; however, only data from olivine-hosted MI in the GPT are reported here. Compositions of MI in the GPT are typically basaltic (average SiO₂ of 49.8 wt %) in the pumices and basaltic-andesite (average SiO₂ of 55.6 wt %) in the scoriae and show a bimodal distribution in most compositional discrimination plots. The compositions of most of the MI in the scoriae overlap with bulk rock compositions of the lavas. Petrological and geochemical evidence suggest that mixing of one or more magmas and/or crustal assimilation played a role in the evolution of the Monte dei Porri magmatic system, especially the GPT. Analyses of the more evolved mineral phases are required to better constrain the evolution of the magma.     

The impacts of the 2004 hurricanes on hydrology, water quality, and seagrass in the Central Indian River Lagoon, Florida

Between August 14 and September 26, 2004, four tropical weather systems (Charley, Frances, Ivan, and Jeanne) affected the central Indian River Lagoon (IRL). The central IRL received a prodigious amount of rainfall for the 2 mo, between 72 and 83 cm, which is a once-in-50-yr rainfall event. High stream discharges were generated that, combined with wind-suspended sediments, significantly reduced salinities and water transparency. In September, salinities among central IRL segments dropped from 30 psu or more to ≤15 psu, color increased from a low of 10 pcu to ≥100 pcu, and turbidity increased from ≤3 NTU up to 14 NTU. Evidence of the hurricanes' physical effects on seagrasses (burial, no scour) was limited to just one of the more than 25 sites inspected. Within 2 to 3 mo following the hurricane period, most parameters related to water transparency returned to or showed improvement over their prehurricane (February–July 2004) levels. Unseasonably low salinities (<20 psu) and moderately high color (>20 pcu) were observed through spring 2005, largely attributable to a relatively long residence time and a wetter-than-average spring season in 2005. By the end of the study period (July 2006), the central IRL generally showed a continuation of two opposite seagrass trends—an increase in depthlimit coverage but a decline in coverage density—that began before 2004. Also, within a limited reach of the central IRL, there was a temporary shift in species composition in summer 2005 (Ruppia maritima increased asHalodule wrightü decreased). It is likely that the persistently low salinities (not color) in 2004–2005 affected the species composition and coverage density. This study reveals that seagrasses are resilient to the acute effects of hurricanes and underscores the need to reduce chronic, an thropogenic effects on seagrasses.     

A comparison of photolysis rate parameters estimated from measured and simulated actinic flux for wintertime conditions at Storm Peak Laboratory,Colorado

Photolysis rate parameters depend upon solar actinic flux and chemical species dependent quantum yields and cross sections. Spectrally resolved measurements of actinic flux should be preferred over flux derived from models for the analysis of field observations. Actinic flux can be difficult to derive from the irradiance measurements of flat-plate radiometers. It is also difficult to estimate from models due to uncertainties in the ozone column, aerosol concentrations and distributions, cloud cover, optical depth and surface albedo. A series of actinic flux measurements were performed at Storm Peak Laboratory (3,210 m above sea level), Colorado, United States with spectroradiometers during the wintertime (January 07–10, 2004). The site is relatively remote with a clean atmosphere and during the wintertime the ground is generally covered by fresh snow with a high albedo. The actinic flux measurements were used to estimate the photolysis rate parameters of ozone, nitrogen dioxide and formaldehyde. The measured actinic flux and the photolysis rate parameters derived from the flux were compared to calculations using the Tropospheric Ultraviolet-Visible Model (TUV), version 4.2 (Madronich and Flocke, 1998). The TUV modeled actinic flux, the measured flux and the photolysis rate parameters derived from them had similar temporal patterns. However there were significant differences in their magnitude due to uncertainties in the data available to initialize the TUV model and the calibration of the spectroradiometer.     

Surface coatings on quartz grains in bentonites and their relevance to human health

The cytotoxicity of quartz in the human lung is recognized to be dependent on both the inherent properties of the silica dust and external factors related to the history of the dust and including the presence of surface contamination. In this study, the physical and chemical surface properties of quartz grains in commercial bentonite deposits from the western (South Dakota) and southern (Alabama) USA were investigated. Measured quartz contents of bentonites range from 1.9 to 8.5 wt% with the <10 μm size fraction comprising 6–45% of this total. Trace element contents (Fe–Ti–Al) of quartz grains from any given bentonite are similar, indicating a single origin for the quartz with little if any contamination from other sources. Surface coatings are pervasive on all quartz grains and resist removal by repeated vigorous washings and reaction with HCl. Textural attributes and XPS and EDS analyses of these coatings are consistent with most being montmorillonite and, less frequently, mixtures of montmorillonite and opaline silica. Opaline silica (opal-A and opal-CT) occurs in two texturally distinct generations: an early massive grain-coating event and as later lepispheres. Montmorillonite coating thicknesses range from <1 μm to more than 10 μm thick. Surfaces of plagioclase, K-feldspar, and biotite grains are conspicuously devoid of montmorillonite coatings, but may show sparse distributions of opal-CT lepispheres. HRTEM has not confirmed a topotactic relationship or atomic structural concordance between montmorillonite coatings and underlying quartz grains. Alternatively, a precursor volcanic glass phase that coats the quartz surfaces during volcanic eruption and/or preferential early precipitation of opaline silica on quartz may provide substrates for development of montmorillonite coatings. Estimations of montmorillonite biodurability under pulmonary pH conditions suggest possible prolonged sequestration of respired bentonite quartz grains from contact with lung materials and modified cytotoxic reactivity.     

Decadal-scale autumn temperature reconstruction back to AD 1580 inferred from the varved sediments of Lake Silvaplana (southeastern Swiss Alps)

A quantitative high-resolution autumn (September-November) temperature reconstruction for the southeastern Swiss Alps back to AD 1580 is presented here. We used the annually resolved biogenic silica (diatoms) flux derived from the accurately dated and annually sampled sediments of Lake Silvaplana (46°27′N, 9°48′E, 1800 m a.s.l.). The biogenic silica flux smoothed by means of a 9-yr running mean was calibrated (r = 0.70, p < 0.01) against local instrumental temperature data (AD 1864-1949). The resulting reconstruction (± 2 standard errors = ± 0.7 °C) indicates that autumns during the late Little Ice Age were generally cooler than they were during the 20th century. During the cold anomaly around AD 1600 and during the Maunder Minimum, however, the reconstructed autumn temperatures did not experience strong negative departures from the 20th-century mean. The warmest autumns prior to 1900 occurred around AD 1770 and 1820 (0.75 °C above the 20th-century mean). Our data agree closely with two other autumn temperature reconstructions for the Alps and for Europe that are based on documentary evidence and are completely unrelated to our data, revealing a very consistent picture over the centuries.     

Origin of silicic volcanic rocks in Central Costa Rica: a study of a chemically variable ash-flow sheet in the Tiribí Tuff

Chemical heterogeneities of pumice clasts in an ash-flow sheet can be used to determine processes that occur in the magma chamber because they represent samples of magma that were erupted at the same time. The dominant ash-flow sheet in the Tiribí Tuff contains pumice clasts that range in composition from 55.1 to 69.2 wt% SiO₂. It covers about 820 km² and has a volume of about 25 km³ dense-rock equivalent (DRE). Based on pumice clast compositions, the sheet can be divided into three distinct chemical groupings: a low-silica group (55.1-65.6 wt% SiO₂), a silicic group (66.2-69.2 wt% SiO₂), and a mingled group (58.6-67.7 wt% SiO₂; all compositions calculated 100% anhydrous). Major and trace element modeling indicates that the low-silica magma represents a mantle melt that has undergone fractional crystallization, creating a continuous range of silica content from 55.1-65.6 wt% SiO₂. Eu/Eu*, MREE, and HREE differences between the two groups are not consistent with crystal fractionation of the low-silica magma to produce the silicic magma. The low-silica group and the silicic group represent two distinct magmas, which did not evolve in the same magma chamber. We suggest that the silicic melts resulted from partial melting of relatively hot, evolved calc-alkaline rocks that were previously emplaced and ponded at the base of an over-thickened basaltic crust. The mingled group represents mingling of the two magmas shortly before eruption. Electronic supplementary material to this paper can be obtained by using the Springer LINK server located at http://dx.doi.org/10.1007/s00445-001-0188-8.