基于QuickBird影像上珊瑚礁发育状况监测实验研究

珊瑚礁生态系统具有很高的生物多样性和重要的生态功能。20世纪80年代以后全球范围内珊瑚礁的大面积退化引起了人们广泛的关注。利用高分辨多光谱QuickBird影像,基于珊瑚礁几何参数与发育指数对南沙典型岛礁发育进行遥感监测,在珊瑚礁体迎风面水域由破波形成的纹理特征表明了海面粗糙度与水下礁坪的发育特性。结果表明,南沙岛礁受地质与气候的影响发育程度不一,半月礁发育较好,发育指数为0.38,仙娥礁发育指数为0.27。建立几何参数等指标能够监测南沙珊瑚礁的发育状况,可对珊瑚礁的保护提供科学依据。     

Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.     

济南东部埠村超单元及其就位机制

分布于济南东部六山圈—大有一带的侵入岩,为燕山早期同一岩浆热事件多次侵入形成的杂岩体,依其形成顺序可划分为山张庄、西杜、大有、赵家鹊山四个单元,归并为埠村超单元。该超单元的形成与幔源基性岩浆上侵及同源岩浆分异产生的中偏基性—中性岩浆多次侵入有关,属热气球式膨胀与火山口塌陷复合就位机制。     

滨州市北部沿海未利用地开发利用探讨

滨州市北部沿海地区土地资源丰富,未利用地较多且集中连片,具有很大的开发利用价值。深入分析滨州市北部沿海地区未利用地特点和近几年开发利用现状,提出开发中应注意加快土地利用的总体规划修编和各专项规划的衔接、抓紧开展土地利用集约评价工作、招商引资与以地选商并重、创新开发模式实行高效开发等问题,充分利用地区优势做好滨州市未利用地开发工作。     

Parabolic Wave Propagation Modelling in Orthogonal Coordinate Systems

This paper presents a numerical model study of the propagation of water waves using the parabolic approximation of the mild-slope equation in the orthogonal coordinate system. Two types of coordinate systems are studied: (a) a general form of orthogonal coordinate system and (b) the conformal system, a special form of orthogonal coordinate system. Two typical examples, namely, expanded breakwaters and a circular channel, are studied to validate the model. First, the examples are studied by use of the general orthogonal coordinates. Then the same examples are computed by use of the confonnal system. The computational results show that the confonnal coordinate system generally gives better predictions than the general orthogonal system. A numerical technique for generating the conformal grid is combined with the numerical model to improve the practicability of the model. The comparison between the result from the numerical grid system and that from the analytical grid system shows that reliable computational results can be obtained by use of the numerical confonnal grid system.     

宽体沙鳅(Botia reevesae)β-肌动蛋白基因的cDNA克隆与表达分析

利用RT-PCR和快速扩增cDNA末端(rapid amplification of cDNA ends,RACE)技术首次克隆了宽体沙鳅(Botia reevesae)-肌动蛋白基因的cDNA全序列,该序列全长为1795bp,由长100bp的5非翻译区(untranslated region,UTR)、570bp的3非翻译区和1125bp的开放阅读框(open reading frame,ORF)组成,编码375个氨基酸。宽体沙鳅-actin氨基酸序列包含1个糖基化位点、9个N-豆寇酰化位点、3个actin信号位点等主要结构区域。PSI-BLAST比对表明,宽体沙鳅-actin氨基酸与真鲷、罗非鱼、虹鳟等鱼类同源性达99%。NJ法系统进化分析显示宽体沙鳅-actin首先与鲢聚在一起,然后与、尖头等鱼类聚在一起。荧光定量PCR检测-actin基因在宽体沙鳅脑、鳃、心脏、肝、胃等12个组织的表达无显著差异(P<0.05),具有良好的稳定性。     

深化改革创新管理全面开创事业单位工作新局面--王文升同志在厅属事业单位工作会议上的讲话

(2005年6月30日)同志们:厅党组对事业单位的发展和这次会议十分重视。在年初召开的全省国土资源局长会议上,厅党组对事业单位的工作已提出了一些要求,作出了一些部署,也请事业单位的代表作了典型发言。厅党组原计划在三四月份召开这次事业单位工作会议,但由于厅机关及各事业单位都在开展保持共产党员先进性教育活动,因此推到了现在。这次会议的主要任务是,总结交流事业单位2004年的工作经验,通报表彰2004年度山东省国土资源管理服务先进单位,学习传达全省事业单位改革工作会议精神,安排部署2005年的工作任务。下面,根据厅党组研究的意见,我…     

盐湖卤水数据采集系统中的通信方法

介绍了盐湖卤水动态自劝观测系统中单片机数据采集系统与智能测钾仪及智能水位计之间的通信。前者来用并行通信，后者采用串行通信．分别介绍了两种通信方式的硬件接口和程序设计。     

包兰铁路沙坡头段不同编制结构的枝条阻沙栅栏防护效应风洞模拟

对包兰铁路沙坡头段枝条阻沙栅栏流场结构进行风洞模拟试验。结果表明:气流在经过横向、竖向阻沙栅栏时均出现了明显的流速分区。气流在经过阻沙栅栏时速度会减弱,但是竖向枝条阻沙栅栏的综合阻风防沙效果明显优于横向阻沙栅栏,同时竖向枝条阻沙栅栏制作上具有工序简单、原材料广泛及不受枝条长度限制的特性,因此,在防沙治沙应用中,竖向枝条阻沙栅栏更适合推广。虽然孔隙度是阻沙栅栏设计的重要技术参数,但栅栏结构对风沙流场产生直接影响,是决定着其对风沙活动防护效应高低的关键因子。     

射电天文台址电子设备电磁辐射评估

射电望远镜具有极高的系统灵敏度,且系统内、系统间及台址内电子设备众多,电磁环境复杂,科学合理地评估台址内电子设备辐射发射对射电天文观测的影响,对系统电磁兼容性设计、无线电管理、屏蔽改造等有重要的指导意义。浅析了射电望远镜系统灵敏度及射电天文领域仪器设备辐射发射相关评估标准;基于射电望远镜系统灵敏度及观测需求,计算了南山25 m射电望远镜馈源口面干扰电平限值,并给出了天线旁瓣增益的计算方法;提出一种基于干扰电平限值、旁瓣增益、干扰测量、路径衰减的电子设备电磁辐射评估方法,并针对南山25 m射电望远镜天线驱动电磁辐射进行了评估,给出了屏蔽需求。