The requirements for stable metallothionein clusters examined using synthetic lobster domains.

Metallothioneins (MTs) are small, cysteine-rich proteins which detoxify xenobiotic metals such as cadmium (Cd) and mercury (Hg). In crustaceans and mammals they consist of two independent domains which are folded around metal-thiolate clusters. MT clusters of different origins, exhibiting distinct, highly conserved cysteine positions on their sequences, show differences in metal-cysteine coordination and reactivity. Lobster-MT, containing two Cd3 beta domains, is an important model for structure-function relationships among the clusters. The influence of (1) the position of the cysteine residues and (2) steric and electrostatic effects of neighboring amino acids on the folding and stability of MT cluster were investigated. Thus, the native lobster beta C and beta N domains (each having nine cysteines and binding three M2+ ions) and a modified domain Cd3 beta C-->N, in which the cysteines of the C-terminal domain were relocated to match the positions of those in the N-terminal domain, were chemically prepared and characterized. The synthetic native domains (Cd3 beta C and Cd3 beta N) were found to exhibit spectroscopic properties, metal-binding affinities and kinetic reactivity similar to the holo-protein. However, the modified Cd3 beta C-->N domain was unusually reactive and in the presence of Chelex, metal chelation resin, aggregated to a Cd5(beta C-->N)2 dimer, which exhibited unusual structure as observed by its 113Cd-nuclear magnetic resonance. These differences in structure and reactivity demonstrated that the requirements for formation of a stable Cd3S9 beta-cluster are more stringent than simply the sequential positions of the cysteines along the peptide chain and must include interactions involving neighboring, noncysteine amino acids.     

某核电厂附近海域生态环境特征及潜在致灾生物研究

为了了解某核电厂周边海域的生态环境状况,筛选潜在威胁核电厂冷源安全的致灾生物,分析影响致灾生物时空分布的主要环境因素,本研究于2018年进行了4个季节的生态调查。结果表明,研究海域浮游植物密度秋季最高,春季优势种为东海原甲藻(Prorocentrumdonghaiense),其他季节优势种为中肋骨条藻(Skeletonema costatum)等硅藻。浮游动物密度夏季最高,以桡足类为主,春、夏季优势种还包括球型侧腕水母(Pleurobrachiaglobosa)和薮枝螅水母(Obeliasp.)。底栖动物的密度和生物量在秋季最高,优势种主要为豆形短眼蟹(Xenophthalmus pinnotheroides)、长吻沙蚕(Glycera chirori)和丝异须虫(Heteromastusfiliforms)。浮游植物、浮游动物和底栖动物群落均在夏季最为稳定,群落多样性水平和物种丰度较高,且分布较为均匀。浮游植物群落的细胞丰度与悬浮物和磷酸盐的浓度正相关性最高。浮游动物的密度受温度和盐度的影响较大。底栖动物的群落分布主要受悬浮物和无机营养盐的浓度影响。本研究共筛选出17种威胁该核电厂冷...     

Input of atmospheric lead to marine sediments in a south-east Pacific coastal area (approximately 36 degrees S)

Atmospheric input of Pb to coastal sediments in the south-east Pacific (approximately 36 degrees S) was estimated using: (1) a salt marsh (non-local emission sources) as a natural collector of atmospheric fluxes and (2) Pb concentrations in rain and air samples, both considered to be representative of the atmospheric input in the study area. A radioisotopic geochronology technique ((210)Pb) was used to estimate the total Pb atmospheric supply to the sediments. The results show that atmospheric input to Concepción Bay accounts for 13-68% of Pb in near shore sediments, evaluated through salt marsh and rain, both showing comparable results. Consequently, there are other relevant Pb sources to explain the higher concentrations in this area. Sediments in the shelf are subject to important influence of upwelling waters, estimated by Salamanca [Sources and sinks of (210)Pb in Concepción Bay, Chile (1993) PhD thesis, Marine Science Research Center, State University of New York at Stony Brook, USA] using (210)Pb. The atmospheric input, however, is mainly responsible for the total Pb input, since the salt marsh (natural atmospheric collector) shows similar Pb(xs) inventories than the shelf, corresponding to a regional-scale Pb emissions.     

Geologic evolution of the Canarian Islands of Lanzarote, Fuerteventura, Gran Canaria and La Gomera and comparison of landslides at these islands with those at Tenerife, La Palma and El Hierro

In this paper we discuss the results of a swath bathymetric investigation of the Canary archipelago offshore area. These new data indicate that volcanism is pervasive throughout the seafloor in the region, much more that would be suggested by the islands. We have mapped tens of volcanic edifices between Fuerteventura and Gran Canaria and offshore Tenerife, La Gomera, El Hierro and La Palma. Volcanic flows are present between Tenerife and La Gomera and salic necks dominate the eastern insular slope of La Gomera. This bathymetry also supports land geologic studies that indicate that the oceanic archipelago has acquired its present morphology in part by mass wasting, a consequence of the collapse of the volcanic edifices. In the younger islands, Tenerife, La Palma and El Hierro, the Quaternary (1.2 to 0.15 Ma) debris avalanches are readily recognizable and can be traced offshore for distances measured in tens of km. Off the older islands, Lanzarote, Fuerteventura, Gran Canaria and La Gomera (<20 to 3.5 Ma), the avalanches have been obscured by subsequent turbidity current deposition and erosion as well as hemipelagic processes. The failure offshore western Lanzarote is in the form of a ramp at the base of the insular slope bound on the seaward side by a scarp. Its size and the lack of evidence of rotation along its landwards side precludes the possibility that it is a slump. It probably represents a slide whose outer scarp is caused by break-up of the slide. Mounds on the ramp’s surface may represent post-displacement volcanic structures or exotic blocks transported to their present locations by the slide. The failures offshore Fuerteventura are so large that, although they occurred in the Miocene-Pliocene, exotic blocks displaced from upslope are still recognizable in the insular margin morphology. The Canary Island insular margin appears to be a creation of Miocene-Pliocene mass wasting and more recent turbidity current deposition and erosion, and hemilepagic deposition. Failures offshore La Gomera are due to debris flows and/or turbidity currents. These events have obscured earlier mass wasting events. An erratum to this article is available at .     

西沙群岛龙介虫科两新种

龙介虫科Serpulidae的盘管虫Hydroides栖于石灰质管内,又名石灰虫,营附着生活，是有害附着生物之一。它们大量附着在船底,影响航行速度;附着在软体动物的重要养殖种类(珍珠贝、鲍、牡蛎等)的贝売上面,影响贝类生长,危害贝类养殖事业。因此,进行龙介虫科分类,生态的研究,可为有害生物的防除工作提供基本资料。 1956年和1975年,中国科学院海洋研究所在我国西沙群岛考察时,在永兴岛和珊瑚岛采到龙介虫科,盘管虫属两个新种,描述如下：     

地裂缝场地建筑结构动力响应特征及抗震设防研究

为研究地震作用下地裂缝场地建筑结构的动力响应特征,以西安地裂缝场地为研究背景,分别考虑独立基础、片筏基础、桩基础和桩筏基础上的框架结构,对结构峰值加速度、层间位移等动力响应特征及其影响因素和影响规律进行系统研究。研究结果表明：(1)地裂缝附近场地上的结构加速度响应和层间位移响应具有明显的放大效应,动力响应峰值随着距地裂缝距离的增大逐渐减小,最终趋于稳定,放大效应的影响范围约为24 m,此范围内的结构需提高抗震设防水平;(2)结构的峰值加速度随层高的增大而增大,整体呈现出“S”形,层间位移角随层高的增大先增大后减小,二者均表现出明显的“上盘效应”,即上盘结构响应强于下盘,且随着输入地震动强度的增大,上、下盘结构动力响应差异进一步扩大,上盘效应愈发显著;(3)片筏基础、桩基础和桩筏基础结构的加速度响应接近,而独立基础结构的加速度与前三者差异明显,但随着楼层的增大,基础形式对结构层间位移角的影响逐渐减弱;(4)上、下盘结构的峰值加速度、峰值位移大小及峰值出现的时间等存在一定差异,这是由于地震波经过地裂缝时发生复杂的反射和折射,结构受到非一致性激励造成的。     

阿尔金山北缘喀腊大湾地区早古生代中酸性侵入岩岩石地球化学特征及构造意义

喀腊大湾地区位于阿尔金山脉东北缘,当地早古生代时期构造环境复杂并且伴随剧烈的花岗质岩浆活动.通过对区域内早古生代中酸性侵入岩5个岩体（大平沟岩体、阿北银铅矿岩体、阿北岩体、4337北花岗岩体、喀腊大湾南岩体）的岩石学、岩相学、地球化学特征研究,探讨早古生代区域重大构造事件和构造环境.南部的喀腊大湾南岩体表现低钾性质,其余4个岩体样本为中高钾钙碱性中酸性岩,并且有向高钾演变的趋势,铝饱和指数在1.0~1.1之间,数据统计和分析大部分样品具有I型花岗岩特征,少部分显示了I型向S型过渡的性质,代表了洋壳-陆壳活动性大陆边缘的环境.结合喀腊大湾周边地质背景,本地区应该是阿尔金古洋壳和塔里木地块南北向碰撞作用的火山岛弧区域.     

Microbial release of potassium from K-bearing minerals by thermophilic fungus Aspergillus fumigatus

A strain of thermophilic fungus Aspergillus fumigatus was cultured with K-bearing minerals to determine if microbe-mineral interactions enhance the release of mineralic potassium. Experiments were carried out in two settings, one with the mineral grains and the fungal cells in direct contact, and the other employing a membrane (pore size 0.22 μm) to separate the two. Measurements over a period of 30 days showed that, irrespective of the experimental setup, the concentration of free K in the culture was drastically higher than those in any of the control experiments where no living organism was present. Moreover, the occurrence of mineral-cell physical contact enhanced potassium release by an additional factor of 3 to 4 in comparison to the separation experiments. For contact experiments, Electron Probe Microanalysis revealed the formation of mycelium-mineral aggregates, and Atomic Force Microscopy imaging further indicated the possible ingestion of mineral particles by the fungus cells. Contrasting to what was observed and expected in control experiments, the potassium solubilization rate showed a positive dependence upon pH when fungi and minerals were mixed directly, and exhibited no correlations with solution acidity if cell-rock contact was restrained. These results appear to suggest that A. fumigatus promoted potassium release by means of at least three likely routes, one through the complexation of soluble organic ligands, another appealing to the immobile biopolymers such as the insoluble components of secretion, and the third related to the mechanical forces in association with the direct physical contact between cells and mineral particles.     

基于动力学震源模型的三维沉积盆地直下型断层地震动模拟

沉积盆地与近断层地震共同作用会增加地震破坏的风险水平,尤其是盆地下方直下型断层发震情况。采用动力学震源模型刻画断层破裂发震过程,开展沉积盆地直下型断层谱元法地震动模拟研究,探讨不同断层面初始剪应力和成核区位置下三维沉积盆地地表响应规律。研究结果表明,断层面应力降对盆地地表地震动的影响显著,在断层面强度一定的情况下,随着初始剪应力的增大,即应力降增大,盆地地表峰值响应增大,原因在于应力降的改变影响了断层破裂释放能量,进而引起断层破裂速度改变,最终导致盆地地表响应发生变化;改变断层面成核区位置会对盆地内部地震动分布规律产生影响,当成核区位置从断层中间向断层左侧移动时,盆地左侧地震动逐渐减小,而右侧地震动逐渐增大,最终表现为盆地右侧地震动显著高于盆地左侧,原因在于改变成核区位置后,导致近断层地震动的方向性效应发生变化。     

中国省会城市国际化水平比较研究

城市国际化不仅是一个结果更是一个与其他国际城市逐步接轨的过程,在此过程中,通过与其他城市的物质、文化与信息的交流,实现城市发展利益的最大化。在目前城市国际化比较研究的基础上,针对现有方法的不足,运用因子分析法,进行了分因素排名比较分析和综合得分排名的分类比较分析,最后根据综合得分通过类间距法将所有省会城市分为三类,并提出各类城市在不同城市国际化发展阶段应选择不同的发展策略。