The requirements for stable metallothionein clusters examined using synthetic lobster domains.

Metallothioneins (MTs) are small, cysteine-rich proteins which detoxify xenobiotic metals such as cadmium (Cd) and mercury (Hg). In crustaceans and mammals they consist of two independent domains which are folded around metal-thiolate clusters. MT clusters of different origins, exhibiting distinct, highly conserved cysteine positions on their sequences, show differences in metal-cysteine coordination and reactivity. Lobster-MT, containing two Cd3 beta domains, is an important model for structure-function relationships among the clusters. The influence of (1) the position of the cysteine residues and (2) steric and electrostatic effects of neighboring amino acids on the folding and stability of MT cluster were investigated. Thus, the native lobster beta C and beta N domains (each having nine cysteines and binding three M2+ ions) and a modified domain Cd3 beta C-->N, in which the cysteines of the C-terminal domain were relocated to match the positions of those in the N-terminal domain, were chemically prepared and characterized. The synthetic native domains (Cd3 beta C and Cd3 beta N) were found to exhibit spectroscopic properties, metal-binding affinities and kinetic reactivity similar to the holo-protein. However, the modified Cd3 beta C-->N domain was unusually reactive and in the presence of Chelex, metal chelation resin, aggregated to a Cd5(beta C-->N)2 dimer, which exhibited unusual structure as observed by its 113Cd-nuclear magnetic resonance. These differences in structure and reactivity demonstrated that the requirements for formation of a stable Cd3S9 beta-cluster are more stringent than simply the sequential positions of the cysteines along the peptide chain and must include interactions involving neighboring, noncysteine amino acids.     

藻类中植物生长素的研究

植物生长素(auxins)是指能引起与(吲哚乙酸IAA)有同样作用的物质的总称(山田长雄等,1997),作为植物激素(phytohormones)的一种,它广泛存在于水生、陆生植物中,对其生产发育起着至关重要的生物活性作用。相对于高等植物,藻类植物生长素的研究起步较晚。本文综合了国内、外相关文献,对藻类中的植物生长素的研究作了较为全面系统的综述。     

僧帽牡蛎二倍体和三倍体卵母细胞发育的超微结构比较

本文利用电子显微镜技术观察僧帽牡蛎二倍体和三倍体初级卵母细胞发育过程中细胞和细胞器的超微结构变化,结果表明：二倍体和三倍体卵母细胞发育差异明显。在卵黄形成期,二倍体卵母细胞呈椭圆形,细胞核呈伪足状突起,核仁呈颗粒状聚合体,细胞器多发达,合成卵黄粒我,细胞膜外有生绒毛,卵母细胞生物合成旺盛、代谢活动强;而三倍体卵母细胞大都呈不规则形状,该仁小、致密,细胞器少且不发达,卵黄粒少,细胞膜外无微绒毛,卵母细胞生物合成及代谢弱。     

基于天地图的农业农村信息共享服务模式

针对省级农业农村厅专题数据较多且无统一规范、分布广泛、信息孤岛化、数据更新手段落后、跨部门共享困难等问题,在收集、整合、分析宏观分布的业务信息和应用需求基础上,利用地理信息、分布式多源信息集成、海量数据的存储、处理与分析等技术,开展农业农村地理信息共享服务模式研究,设计并实现了省级农业地理信息共享服务平台,包括门户、一...     

加强目标观测,服务防灾减灾

以深入浅出的方式介绍了目标观测的定义、历史、确定目标观测敏感区的方法,以及实施目标观测与防灾减灾的关系;并以欧洲中期天气预报中心目标观测业务化为例,简明地概括了目标观测的实施过程;结合国内目标观测研究现状,对中国目标观测的未来提出了看法。     

风生涡旋对热盐环流年代际变率的影响——基于盒子模型的分析

大洋热盐环流（Thermohaline Circulation,简称THC）是主要源于北大西洋的深海环流,对全球经向水和热平衡具有重要的作用,是影响长期气候变化的一个重要因素。采纳了Longworth等的观点,通过添加扩散项的形式在经典的Stommel盒子模型中引进了风驱洋涡（Wind-Driven Ocean Gyre,简称WDOG）,并借鉴Sun等的研究方法,讨论了WDOG对THC年代际变率的影响。通过数值计算发现,WDOG的引入会缩短（延长）处于TH型（SA型）平衡态时的THC在扰动作用下的恢复时间。结合Wu and Mu文章的结论,可知正是由于WDOG对处于不同平衡态下THC的环流强度产生了不同的作用,因而导致了上述现象的出现。     

Detrital zircon geochronology and provenance of sediments within the Mesozoic basins:New insights into tectonic evolution of the Qinling Orogen

Mesozoic sedimentary units within the Nanzhao and Mashiping basins record the paleogeographic and tectonic evolution of the Qinling Orogen (QO). This study uses...     

基于模糊物元模型的中国旅游生态安全评价及障碍因子诊断研究

基于DPSIR模型构建旅游生态安全评价指标体系,利用模糊物元模型对中国31个省(市、区)(不含港澳台)旅游生态安全进行动态评价,并运用空间自相关分析空间格局演化特征.同时借助障碍度模型,对旅游生态安全的障碍因子进行诊断.结果表明:①旅游生态安全呈现出波动增长的"W"型演化趋势.2013-2016年中国旅游生态安全在空间...     

基于动力学震源模型的三维沉积盆地直下型断层地震动模拟

沉积盆地与近断层地震共同作用会增加地震破坏的风险水平,尤其是盆地下方直下型断层发震情况。采用动力学震源模型刻画断层破裂发震过程,开展沉积盆地直下型断层谱元法地震动模拟研究,探讨不同断层面初始剪应力和成核区位置下三维沉积盆地地表响应规律。研究结果表明,断层面应力降对盆地地表地震动的影响显著,在断层面强度一定的情况下,随着初始剪应力的增大,即应力降增大,盆地地表峰值响应增大,原因在于应力降的改变影响了断层破裂释放能量,进而引起断层破裂速度改变,最终导致盆地地表响应发生变化;改变断层面成核区位置会对盆地内部地震动分布规律产生影响,当成核区位置从断层中间向断层左侧移动时,盆地左侧地震动逐渐减小,而右侧地震动逐渐增大,最终表现为盆地右侧地震动显著高于盆地左侧,原因在于改变成核区位置后,导致近断层地震动的方向性效应发生变化。     

Geochemical and Sr-Nd isotopic study of alkaline syenites in Liangtun-Kuangdonggou, Liaoning Province, China： Evidence for enriched mantle before 1.86 Ga and implications

The 1.86 Ga Liangtun-Kuangdonggou complex (LKC) is one of the oldest alkaline syenite bodies so far discovered in China. This syenite suite has elevated contents of total alkali (K2O Na2O), with an average of 10.50%, and a mean Rittmann Index (σ) of 6.48. The intrusions have slightly higher concentrations of K2O than those of Na2O on a weight percent basis, indicating the rocks belong to potassium-rich alkaline syenite series. Total rare-earth element concentrations (∑REE ) of the rocks are relatively high, ranging from 324×10 -6 to 1314×10 -6, with a mean value of 666×10 -6. The REE patterns are subparallel and rightward steep with (La/Yb)N >33, showing mild negative to positive Eu anomalies (δEu: 0.63-1.15). All samples exhibit strong LILE and LREE enrichments and TNT (Nb, Ta, Ti) and P depletions in multi-element spidergrams. On the εSr(t)-εNd(t) correlation diagram, most analytical data points plot within the enriched mantle field with low ( 87Sr/86Sr)i ratios (0.7045-0.7051) and negative εNd(t) values (-3.72--3.97), falling among those kimberlites from Fuxian County, Liaoning Provinve, from Mengyin County, Shandong Province and the Ⅱ-type kimberlites from South Africa. These characteristics imply that the LKC-rocks may have the same source as the above-mentioned kimberlites, i.e., they have close connections to the materials derived from enriched mantle reservoirs, further revealing that the upper mantle beneath the northeastern part of the North China Plate had been highly enriched before 1.86 Ga. Geodynamically, the LKC-rocks were formed in a within-plate environment with close genetic connections to rift-related alkaline magma activities possibly controlled by ancient mantle plumes.