Prüfstrategie zur Bewertung potentiell erbgutverändernder Wasserinhaltsstoffe unter Einsatz gentoxikologischer Methoden

Assessment and Characterization of Genotoxic Water Contaminants with Modern Genotoxicological Methods The necessity of genotoxicological studies in water requires modern and practicable concepts for a prospective environmental protection. Up to now, genotoxicological studies prefer only the observation and detection of basic genotoxic effects in enriched surface water samples and the fruitless search for the involved water contaminants. In this publication, a new test strategy for the detection and the assessment of genotoxicity in water will be introduced. This strategy allows the detection and the verification of positive results and is therefore able to assess the evidence of these results for the water quality more accurate. The aim of this strategy is the detection of basic genotoxic effects (primary DNA damages), the clarification of the biological mechanisms, and the search for the reasons with modern analytical methods. The involved water contaminants and their mode of action will then be better understood. A test strategy in three steps will be proposed. In the first step genotoxicological indicator tests such as comet assay, alkaline filter elution, or the SOS-umu-test are used to get information about the basic status of the tested water samples. In the second step, the positive results of the primary DNA damages will be verified with mutation tests such as the micronucleus assay. The involved biological mechanisms will be biochemically characterized, and the biological active substances will be preclassified. Finally, in the third step the observed mechanisms will be confirmed with modern chemical methods such as HPLC MS-MS. These modern techniques are also able to detect and quantify the genotoxic compounds preclassified in step two.     

Effect of bed load supply on sediment transport in mountain streams

Mountain streams with their tributary torrents build the upper part of the fluvial network. They are important regarding the transfer of sediment from headwaters to lower basins. Channels are typically steep with wide grain size distributions, ranging from fine sand up to large boulders, and a stabilized bed surface. Mountain streams often are supply-limited with respect to mobile bed load, which needs to be addressed when bed load transport equations are applied to such streams. To better understand supply limitation, laboratory experiments highlighting the effect of bed load supply on incipient motion and bed load transport rate are discussed. Experimental tests were done in which fine bed load was supplied to a previously armored channel bed, with flow conditions ranging from one-third to twice the critical dis-charge for the bed surface. At flows not exceeding the critical discharge, the time series of the bed load transport rate at the downstream model boundary featured consistent patterns which are attributed to distinct phases: (i) a temporal lag, (ii) an equilibrium state, and (iii) a post-supply phase. Bed load transport occurred even at flows distinctly below that for incipient motion of the bed surface. But, with the mass of total bed load outflow approaching the supply amount, the mass did not exclusively consist of supplied grains. The coarser the supplied bed load, the more sediment was mobilized from the bed surface. At higher flows, processes differed. Total bed load outflow exceeded the supply amount and the break-up of the armor layer caused a refining of the bed surface.     

The relevance of bisphenol A adsorption during Fenton’s oxidation

The aim of this research was to assess the efficiency of Fenton’s oxidation for degradation of endocrine disruptor bisphenol A (BPA) with emphasis on extent of accompanying adsorption. Adsorption on the waste sludge resulting from the Fenton’s oxidation could represent a significant impact on the final removal efficiency of BPA. Fenton’s oxidation was accomplished at two concentrations of BPA (0.228 and 22.8 mg L^?1); both at the selected molar ratio of reagents Fe²⁺:H₂O₂ (1:10), as a function of reaction time. The kinetics of adsorption of BPA on waste sludge was determined for the same two concentrations of BPA at two concentrations of waste sludge (0.1 and 6.0 g L^?1). In addition to changing concentrations of BPA and sludge, the adsorption process was also influenced by parameters such as temperature, pH and contact time. Adsorption isotherms were determined. Oxidation and adsorption were monitored by gas chromatography combined with mass spectrum. It has been confirmed that BPA is not completely oxidized in Fenton’s oxidation, because it is adsorbed to formed waste ferric sludge and thus necessary precautions for sludge deposition must be observed.     

Behaviour of zircon in high-grade metamorphic rocks: evidence from Hf isotopes,trace elements and textural studies

Hf isotopic data of minerals in a mafic pyroxene granulite from the southern Bohemian Massif, together with their major and trace element composition and petrological observations were used to decipher the metamorphic history and behaviour of zircon in the granulite. The Hf isotopic composition in the minerals was used to estimate whether the decompression reaction, namely the consumption of garnet and rutile, could have provided Zr for the formation of newly grown metamorphic zircon. The age of the decompression reaction indicated by the evolution of Hf isotopes in garnet and orthopyroxene is between 333 and 331 Ma, i.e. ca. 7 Ma younger than the available U–Pb zircon ages from the Moldanubian granulites and than the newly obtained 343 ± 2 Ma laser ablation ICP-MS U–Pb age of zircons. The combination of bulk and in-situ Hf isotopic data, major and trace element composition and petrological modeling of P–T evolution revealed that the formation of zircons can not be related to the decompression phase of the evolution of the mafic granulites. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

Environmental changes and the Migration Period in northern Germany as reflected in the sediments of Lake Dudinghausen

Paleolimnological techniques were used to identify environmental changes in and around Lake Dudinghausen (northern Germany) over the past 4800 yr. Diatom-inferred total phosphorus (DI-TP) changes identify four phases of high nutrient levels (2600-2200 BC, 1050-700 BC, 500 BC-AD 100 and AD 1850-1970). During these high DI-TP phases, fossil pollen, sediment geochemistry and archaeological records indicate human activities in the lake catchment. Although the same paleo-indicators suggest increased human settlement and agriculture activity during the late Slavonic Age, the Medieval Time and the Modern Time (AD 1000-1850), DI-TP levels were low during this period. In the sediments, iron and total phosphorus were high from ∼AD 100 to 1850, likely due to increased inflow of iron-rich groundwater into the lake. Increased iron input would have lead to a simultaneous binding and precipitation of phosphate in the upper sediment and overlying water column. As a result, anthropogenic impact on Lake Dudinghausen was masked by these phosphorus-controlling processes from AD 1000 to 1850 and was not evident by means of DI-TP. In accordance with fossil pollen, sediment geochemistry and limited archaeological records, DI-TP levels were low from AD 100-1000. Groundwater levels likely rose during this period as the climate gradually changed toward colder and/or moister conditions. Such climate change likely led to reduced settlement activities and forest regeneration in the catchment area. Our results are concordant with similar studies from central Europe which indicate rapid decreasing settlement activities from AD 100 to 1000.     

Water and sediment quality assessment in the mid-Black Sea coast of Turkey using multivariate statistical techniques

The aim of this study was to investigate the water and sediment quality in the mid-Black Sea coast of Turkey. The samples were collected from six stations during 2007. Investigated parameters were total carbon (TC), total inorganic carbon (TIC), total organic carbon (TOC), ammonium-nitrogen (NH₄-N), nitrate-nitrogen (NO₃-N), nitrite-nitrogen (NO₂-N), total phosphorus (TP), sulphate, total hardness, methylene blue active substances (MBAS), phenol, adsorbable organic halogens (AOX), dissolved oxygen (DO), pH and electrical conductivity (EC) in water samples and TC, TIC, TOC, TP, pH, electrical conductivity (EC), redox potential (Eh) and water content (WC) in sediment samples. Different multivariate statistical techniques were used to evaluate variations in surface water and sediment quality. Principal component analysis helped in identifying the factors or sources responsible for water and sediment quality variations. Five factors were found responsible for 87.63% of the total variance in the surface waters. In sediments, three factors explained 84.73% of the observed total variance. Cluster analysis classified the monitoring sites into two groups based on similarities of water and sediment quality characteristics.     

Seepage problems in the karstic limestone foundation of the Kalecik Dam (south Turkey)

Seepage phenomena through the karstic limestone foundation of Kalecik Dam are investigated. The dam, designed as a rock-fill dam with a height of 77 m, is already used for irrigation. The foundation consists of Mesozoic ophiolite, Paleocene allochthonous units composed of different lithologies, and Miocene conglomerate. The conglomerate lies unconformably on the other units. Quaternary basaltic lava patchily covers the others. Seepage from upstream to downstream occurs through the allochthonous karstic limestone. This limestone, which is overlain by conglomerates, has a thickness increasing towards the right abutment.

To prevent seepage at the right abutment, a 200-m long and 60-m deep grout curtain along the dam axis was constructed. After the impoundment, some springs occurred downstream, and for this reason, extra grouting was performed. However, the seepage problem could not be solved. In order to determine the seepage direction and karstification pattern, hydrological studies have been done. Additional investigation boreholes have been drilled to observe fluctuations of the groundwater level and to analyze hydrochemistry. Also, dye tracer tests have been carried out.

As a result of these hydrogeological studies, seepage paths were observed in the karstic limestone located between the dam and the spillway. As those carbonate rocks continue beneath the spillway, the seepage problems are enjected to continue.     

Semantic Enablement for Spatial Data Infrastructures

Building on abstract reference models, the Open Geospatial Consortium (OGC) has established standards for storing, discovering, and processing geographical information. These standards act as a basis for the implementation of specific services and Spatial Data Infrastructures (SDI). Research on geo‐semantics plays an increasing role to support complex queries and retrieval across heterogeneous information sources, as well as for service orchestration, semantic translation, and on‐the‐fly integration. So far, this research targets individual solutions or focuses on the Semantic Web, leaving the integration into SDI aside. What is missing is a shared and transparent Semantic Enablement Layer for SDI which also integrates reasoning services known from the Semantic Web. Instead of developing new semantically enabled services from scratch, we propose to create profiles of existing services that implement a transparent mapping between the OGC and the Semantic Web world. Finally, we point out how to combine SDI with linked data.     

Retrieval of chlorophyll and nitrogen in Norway spruce (Picea abies L. Karst.) using imaging spectroscopy

The research evaluated the information content of spectral reflectance (laboratory and airborne data) for the estimation of needle chlorophyll (C_AB) and nitrogen (C_N) concentration in Norway spruce (Picea abies L. Karst.) needles. To identify reliable predictive models different types of spectral transformations were systematically compared regarding the accuracy of prediction. The results of the cross-validated analysis showed that C_AB can be well estimated from laboratory and canopy reflectance data. The best predictive model to estimate C_AB was achieved from laboratory spectra using continuum-removal transformed data (R²cv = 0.83 and a relative RMSEcv of 8.1%, n = 78) and from hyperspectral HyMap data using band-depth normalised spectra (R²cv = 0.90, relative RMSEcv = 2.8%, n = 13). Concerning the nitrogen concentration, we observed somewhat weaker relations, with however still acceptable accuracies (at canopy level: R²cv = 0.57, relative RMSEcv = 4.6%). The wavebands selected in the regression models to estimate C_AB were typically located in the red edge region and near the green reflectance peak. For C_N, additional wavebands related to a known protein absorption feature at 2350 nm were selected. The portion of selected wavebands attributable to known absorption features strongly depends on the type of spectral transformation applied. A method called “water removal” (WR) produced for canopy spectra the largest percentage of wavebands directly or indirectly related to known absorption features. The derived chlorophyll and nitrogen maps may support the detection and the monitoring of environmental stressors and are also important inputs to many bio-geochemical process models.     

Coupling sensor observation services and web processing services for online geoprocessing in water dam monitoring

ABSTRACT

Novel sensor technologies are rapidly emerging. They enable a monitoring and modelling of our environment in a level of detail that was not possible a few years ago. However, while the raw data produced by these sensors are useful to get a first overview, it usually needs to be post-processed and integrated with other data or models in different applications. In this paper, we present an approach for integrating several geoprocessing components in the TaMIS water dam monitoring system developed with the Wupperverband, a regional waterbody authority in Germany. The approach relies upon the OGC Web Processing Service and is tightly coupled with Sensor Observation Service instances running at the Wupperverband. Besides implementing the standardized XML-based interface, lightweight REST APIs have been developed to ease the integration with thin Web clients and other Web-based components. Using this standards-based approach, new processing facilities can be easily integrated and coupled with different observation data sources.