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191.
对虾传染性皮下及造血组织坏死病毒衣壳蛋白基因的克隆表达及抗体制备 总被引:2,自引:0,他引:2
本文根据GenBank登录的对虾传染性皮下及造血组织坏死病毒(IHHNV)的全基因组序列(NC-002190),设计出主要结构蛋白基因的相应引物,从厦门发病的对虾中,提取DNA,以此为模板,利用PCR扩增目的基因.再将目的基因分别与pGEX-6p1载体和pET-28a载体连接,构建重组表达载体pGEX-Ⅳ、pET-Ⅳ,重组子经酶切和测序鉴定后导入表达型大肠杆菌BL21,IPTG诱导表达,SDS-PAGE检测,大小约为62KDa和41KDa,与预测大小一致.将pET-Ⅳ表达重组蛋白经纯化,免疫小鼠,Western免疫印迹反应结果表明其抗体均可与pGEX-Ⅳ和pET-Ⅳ表达的蛋白发生特异性反应.本研究的结果为对虾IHHNV的快速检测及其免疫提供了研究基础. 相似文献
192.
对虾传染性肌肉坏死病(infectious myonecrosis,IMN)最初于2002年8月爆发于巴西Piaui州的凡纳滨对虾(Litopenaeus vannamei)养殖场,并很快在巴西东北沿岸蔓延开来,目前已传至亚洲.2004年,经美国亚利桑那大学(University of Arizona)水产病害实验室研究,该病为一种新的对虾疾病,根据其症状,暂定名为传染性肌肉坏死病. 相似文献
193.
On the basis of the soil environment investigation in Da'an City, Jilin Province, China, 40 soil samples from main land use types were obtained and tested by standard method. Soil organic matter (SOM), total N (TN), total P (TP), total K (TK), available N (AN), available P (AP) and available K (AK) were chosen as the evaluation factors. A regional soil nutrient evaluation model was developed based on the matter-element model. The results show that the soil samples with nutrient grade Ⅱ-Ⅴ respectively account for 10%, 30%, 32.5% and 27.5%, and those with grade Ⅳ and Ⅴ account for 60% in all samples. The relationship between soil nutrients and land types indicates that the nutrients of farmland are relatively good, with 41.7% of soil samples with the nutrient grade Ⅳ and Ⅴ. The nutrients of saline-alkali land and sandy land are the worst, with 100% of soil samples with the nutrient grade Ⅳ and Ⅴ. And the ratios of soil samples grade Ⅳ and Ⅴ in grassland and wasteland are respectively 62.5 % and 54.55%. Generally speaking, the soil nutrients status in Da'an City is poor, 60% of soil samples are in poor and extremely poor conditions, indicating that the soil has been severely eroded. Being a relatively superior evaluation method with more accurate resuits and spatial distribution consistency, matter-element analysis is more suitable for regional soil nutrient evaluation than previous models. 相似文献
194.
?????????????????????????1997??11??8?????????????o????Ч?????????????????????????λ?????????Ч????????仯??????????????????????Ч?????????仯????????λ?????????????????????????Ч????????仯??????????λ???????????????????? 相似文献
195.
Tidal current observation in the southern Yellow Sea in the summers of 2001 and 2003 总被引:1,自引:0,他引:1
Direct current observations in the Yellow Sea interior are very scarce due to intense fishing and trawling activities. Most previous studies on tides in the area were based on coastal measurements or satellite altimeter sea levels and have not been rigorously compared with direct measurements. In this paper, tidal currents are studied with current profiles from three bottom-moored Sontek Acoustic Doppler Profilers (ADPs) deployed in the southern Yellow Sea in summer of 2001 and 2003. The measured current series were dominated by tidal currents. Maximum velocities are between 40-80 cm/s at the mooring stations. M2 current is the most dominant primary tidal constituent, while MS4 and M4 are the most significant shallow water tides with much smaller amplitudes than the primary tides. 相似文献
196.
??LAMBDA????????GNSS????????????????????????????????????????????????????????????????????????Ratio??????????????????Ratio?????????????????????L1??L2?????λ????????????????????÷????????????????????????????????????????????????????????? 相似文献
197.
We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future. 相似文献
198.
1 枣庄矿产资源开采史 枣庄市矿产资源的开采有着悠久的历史.据当地甘泉寺的碑碣记载,1308年就有人在该地区挖窑采煤.13世纪中叶,民间采煤业逐渐兴盛起来.明代诗人满碧山"磨塘山欲尽,煤井地皆空"的诗句,就是对枣庄地区的采矿描述.开采陶土烧瓷的历史约有1400年;开采铁矿石手工冶铁也有500多年的历史.改革开放以来,枣庄地区的采矿业有了较快的发展,采矿品种由单一转向多种,现已达15个矿种.全市年产原煤2 000多万吨.县、乡(镇)、村办和个体办采石厂300多家,年采矿石量1 600万t,黏土、河砂等采矿点200余处,全市从事采矿业人员达16万余人. 相似文献
199.
200.
东海东北部春季若干重要水文结构的分析 总被引:7,自引:2,他引:7
本文主要基于韩国海洋研究所在东海沿岸海洋过程试验中收集的CTD资料,分析了1995年春季出现在东海东北部的一些重要水文结构。结果表明,一种锋涡状结构出现在黑潮向东转折点附近。它不仅使邻近海域的水文结构变得更复杂,而且诱发黑潮水与陆架水间活跃的交换。在陆架坡折处观测到若干孤立的陆架水块,可能是锋涡的卷挟作用所致;该海域存在4个水团,即黑潮水、对马暖流水、陆架水和混合水。对马暖流水分为上下两层:上层水为变性黑潮水,盐度比黑潮水约低0.1,底层对马暖流水仅位于冲绳海槽区,并有着与黑潮中层水相同的温、盐特性;一种双锋结构出现在邻近黑潮的陆架边缘附近。在内陆架形成的陆架锋,由北向南伸展时,愈来愈偏向陆架边缘。而黑潮锋沿九州以西深槽的陆架边缘向北伸展。在黑潮转折点附近,两锋几乎合并为一条锋。狭窄的锋带由黑潮水及其变性水和陆架水的混合水所占据。 相似文献