Identification of five sea cucumber species through PCR-RFLP analysis

Sea cucumbers are traditional marine food and Chinese medicine in Asia. The rapid expansion of sea cucumber market has resulted in various problems, such as commercial fraud and mislabeling. Conventionally, sea cucumber species could be distinguished by their morphological and anatomical characteristics; however, their identification becomes difficult when they are processed. The aim of this study was to develop a new convenient method of identifying and distinguishing sea cucumber species. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of mitochondrial cytochrome oxidase I gene (COI) was used to identifing five sea cucumber species (Apostichopus japonicus, Cucumaria frondosa, Thelenota ananas, Parastichopus californicus and Actinopyga lecanora). A 692 bp fragment of COI was searched for BamHI, KpnI, PstI, XbaI and Eco31I restriction sites with DNAMAN 6.0, which were then used to PCR-RFLP analysis. These five sea cucumber species can be discriminated from mixed sea cucumbers. The developed PCR-RFLP assay will facilitate the identification of sea cucumbers, making their source tracing and quality controlling feasible.     

建立基于GIS的浙江省矿产资源储量管理系统

GIS是国土资源信息系统及办公自动化系统的基石 ,亦将是国土资源工作的各个领域中最具影响力、应用最广泛的一种技术。本文在分析已有矿产资源含量数据库特点和GIS应用现状的基础上 ,提出了在GIS平台上建立浙江省矿产资源储量管理信息系统的初步设想 ,概要论述了系统的功能、软件流程和系统结构     

超导重力仪GWR-TT70在武昌站的测量结果及其长期稳定性分析

介绍了超导重力仪 GWR- TT70 ( 1986- 0 1～ 1993- 12 )在武昌站的观测数据及潮汐参数的计算结果。采用了非数字滤波调和分析法 ,计算出该仪器观测重力值对气压的响应系数和长周期波潮汐参数 ,得到潮汐因子在 8年中的稳定度为 0 .5%以内。最后分析了仪器漂移特征和武昌站重力长期变化的可能量级。     

活性白云粉生产工艺及其物化性能研究

活性白云粉（ABP）是在白云岩经过破碎、细粉碎、表面改性的基础上得到的一种新型改性功能填料。本文利用河南辉县市的拍石头和张村两地产出的白云岩作为实验原料，对其表面改性的实验方法、改性剂选择及用量、改性条件及改性效果等方面进行了一定的探讨，并且对改性前后的白云石粉体的粒度、比表面积等性能及红外光谱特征做了对比研究。生产工艺和物性测试研究表明：活性白云粉代替轻质碳酸钙作橡胶填料前景广阔；与轻钙相比，活性白云粉的粒度不够均匀，经优化和改善生产工艺条件，可望进一步提高其应用价值。     

Marine bacterium strain screening and pyrethroid insecticide-degrading efficiency analysis

A pyrethroid insecticide-degrading bacterium, strain HS-24, was isolated from an offshore seawater environment. The strain, which can degrade cypermethrin(CYP) and deltamethrin(DEL), was identified as Methylophaga sp. The optimal culture and degradation conditions for CYP and DEL by strain HS-24 is pH 7 at 28°C. Under optimum culture conditions, strain HS-24 exhibited a broad degradation concentration range of 100, 200, 400, 600, and 800 mg/L for CYP and DEL. The metabolic intermediates were analyzed by NMR, which provided strong evidence that CYP and DEL removal occurred mainly because of a biological process. The toxicity of the degradation products of strain HS-24 was studied simultaneously by measuring the light output of the luminescence bacterium. This demonstrated that the biodegradation ability of strain HS-24 significantly decreased the toxicity of CYP- and DEL-contaminated aquaculture seawater. Finally, the findings of this paper indicate that strain HS-24 is thus revealed as a biological agent for the remediation of marine aquatic environments.     

1959-2003年青海省干湿变化分析

利用青海省1959-2003年气象资料,计算了修正的Palmer干旱指数,并对其进行了分析。结果表明：在青海省旱涝监测中,PDSI指数反映旱涝程度更为客观;青海省的干旱主要以轻旱为主;夏秋季年际干湿交替较冬春季频繁,变化振幅也较大;秋季青海省干旱化倾向最为严重,冬春季出现轻旱几率最大。另外,春季干旱总面积在减小;夏季轻旱面积增加,而中旱、重旱面积在减小;秋冬季重旱面积在增加。     

一种以能量平衡为基础的干旱指数

本文以能量平衡公式为基础,根据实际蒸发与潜在蒸发的关系依赖于土壤水分含量的事实,导出一种表达干湿状况的指标——土壤水分干旱指数。     

污染分布下的Lp估计抗差测度

观测数据中含有粗差或异常值扰动的误差分布可视为污染分布。根据M估计理论,导出了Lp估计的影响函数,分析了Lp估计的抗差能力,并用效率来测度估计的优劣、综合抗差能力和效率,指出观测数据中含有粗差的扰动,应用p为[1．2．1．5]的Lp估计来进行处理。     

海南岛三叠纪中基性岩的年代学、地球化学及其地质意义

辉长岩大多为地幔岩石部分熔融的产物,辉绿岩脉及碱性正长岩的形成通常与伸展构造有关,本文对海南岛万宁辉长岩及辉绿岩脉和分界洲正长岩进行了系统的年代学和岩石地球化学研究,并以此来讨论其构造意义。LA-ICP-MS锆石U-Pb定年结果表明,万宁辉长岩及辉绿岩脉形成约在240 Ma,分界洲正长岩形成约在231 Ma,主量元素特征表明万宁辉长岩和辉绿岩分别属碱性系列和亚碱性系列,分界洲正长岩属于典型的碱性岩浆岩。万宁辉长岩及辉绿岩脉的稀土元素具有轻稀土富集的特点(LREE/HREE=7.22～8.50和8.11～11.10),微量元素具有岛弧型火山岩的特征,富集大离子亲石元素K、Rb、Ba、Th,贫高场强元素Nb、Ta和Zr、Hf;分界洲正长岩的微量元素特征显示出与A型花岗岩类似的特征。海南岛三叠纪中基性岩如分界洲正长岩和万宁辉长岩及辉绿岩脉形成的构造背景为陆内伸展环境,指示海南岛在240～230 Ma处于印支造山运动的应力松弛阶段。