The dimensions of sand ripples in full-scale oscillatory flows

New large-scale experiments have been carried out in two oscillatory flow tunnels to study ripple regime sand suspension and net sand transport processes in full-scale oscillatory flows. The paper focuses on ripple dimensions and the new data are combined with existing data to make a large dataset of ripple heights and lengths for flows with field-scale amplitudes and periods. A feature of the new experiments is a focus on the effect of flow irregularity. The combined dataset is analysed to examine the range of hydraulic conditions under which oscillatory flow ripples occur, to examine the effects of flow irregularity and ripple three-dimensionality on ripple dimensions and to test and improve existing methods for predicting ripple dimensions.The following are the main conclusions. (1) The highest velocities in a flow time-series play an important role in determining the type of bedform occurring in oscillatory flow. Bedform regime is well characterised by mobility number based on maximum velocity in the case of regular flow and based on the mean of the highest one tenth peak velocities in the case of irregular flow. (2) For field-scale flows, sand size is the primary factor determining whether equilibrium ripples will be 2D or 3D. 2D ripples occur when the sand D₅₀ ≥ 0.30 mm and 3D ripples occur when D₅₀ ≤ 0.22 mm (except when the flow orbital diameter is low). (3) Ripple type (2D or 3D) is the same for regular and irregular flows and ripple dimensions produced by equivalent regular and irregular flows follow a similar functional dependence on mobility number, with mobility number based on maximum velocity in the case of regular flow and based on the mean of the highest one tenth velocities in the case of irregular flow. For much of the ripple regime, ripple dimensions have weak dependency on mobility number and ripple dimensions are similar for regular and irregular flows with the same flow orbital amplitude. However, differences in ripples produced by equivalent regular and irregular flows become significant at the high mobility end of the ripple regime. (4) Ripple dimensions predicted using the Wiberg and Harris formulae are in poor agreement with measured ripple dimensions from the large-scale experiments. Predictions based on the Mogridge et al. and the Nielsen formulae show better overall agreement with the data but also show systematic differences in cases of 3D ripples and ripples generated by irregular flows. (5) Based on the combined large-scale data, modifications to the Nielsen ripple dimension equations are proposed for the heights and lengths of 2D ripples. The same equations apply to regular and irregular flows, but with mobility number appropriately defined. 3D ripples are generally smaller than 2D ripples and estimates of 3D ripple height and length may be obtained by applying multipliers of 0.55 and 0.73 respectively to the 2D formulae. The proposed modified Nielsen formulae provide an improved fit to the large-scale data, accounting for flow irregularity and ripple three-dimensionality.     

Growth and reproduction of the bivalve Spisula subtruncata (da Costa) in Dutch coastal waters

The bivalve Spisula subtruncata is usually abundant in shallow coastal waters along the Dutch coast. However, its biomass has been decreasing since 1995. In order to assess whether reproductive failure may be the cause of the observed decline over the last decades, the energy investment in reproduction of a population of S. subtruncata from central Dutch coastal waters was studied. The population studied consisted of individuals of up to four years old. Shell length reached maximum values of around 32 mm and individual total body, somatic and gonadal ash-free dry mass reached maximum values of about 278 mg AFDM, 252 mg AFDM and 76 mg AFDM, respectively. A clear seasonal cycle in somatic and gonadal mass was observed. Somatic and gonadal mass indices increased in early spring and reached maximum values during summer, followed by a decrease to minimum values at the beginning of the following year. Spawning was in June–July and settlement of spat seems to have occurred in July–August. Mean oocyte diameter was 57.43 ± 0.03 μm, corresponding to a volume of 98972 μm³. These results suggested that reproductive failure was not the cause of the current population decline. Most likely, unsuccessful settlement of spat and/or severe predation during the first months of life were responsible for the observed patterns.     

Induction of vitellogenin in vivo and in vitro in the model teleost medaka (Oryzias latipes): comparison of gene expression and protein levels

Quantification of the egg yolk precursor vitellogenin (VTG) in fish has become a standard technique to detect estrogenic effects of known chemicals and environmental samples. In the present study, we have analysed VTG induction by estradiol, ethynylestradiol and genistein exposure in the model teleost medaka (Oryzias latipes) and demonstrate that the medaka is a suitable model system to analyse estrogenic effects. By comparing VTG gene expression and protein levels we show that in principal both techniques can be used to study VTG induction in vivo (juvenile and adult males) and in vitro (primary cultures of male liver cells). If a short term in vivo or in vitro exposure is performed, detection of mRNA might be sufficient. For long term studies with the need to detect weak estrogenic chemicals and a precise quantification, immuno-chemical detection may be favoured.     

Upper temperature tolerances of exotic brackish-water mussel, Mytilopsis leucophaeata (Conrad): an experimental study

The dark false mussel, Mytilopsis leucophaeata is an important mussel colonising the brackish-water systems of temperate and subtropical regions. Of late it has earned notoriety as a biofouling species in industrial cooling water systems. However, there are no published data on the temperature tolerance of this species. This paper presents data on the upper temperature tolerance of this mussel from the view point of biofouling control using thermal methods. In addition to mortality, response of physiological activities such as oxygen consumption, filtration rate, foot activity and byssus thread production were also studied at temperatures varying from 5 to 35 degrees C. Experiments were also carried out to understand the effect of mussel size, breeding condition, nutritional status and acclimation conditions (temperature and salinity) on the mortality pattern. The physiological activities were significantly reduced at temperatures beyond 27.5 degrees C and ceased at 35 degrees C. In 20 mm size group mussels exposed to 37 degrees C, 50% mortality was observed after 85 min and 100% mortality after 113 min. The effect of mussel size on mortality at different temperatures was significant, with the larger size group mussels showing greater resistance. M. leucophaeata collected during the non-breeding season (December-April) were more tolerant to temperature than those collected during the breeding season (June-October). Nutritional status of the mussel had no significant influence on the thermal tolerance of the mussel: fed and starved (non-fed) mussels succumbed to temperature at comparable rates. The effect of acclimation temperature and acclimation salinity on M. leucophaeata mortality at different temperatures was significant. Survival time increased with increasing acclimation temperature and decreased with increasing salinity. In comparison with other co-occurring species such as Mytilus edulis and Dreissena polymorpha, M. leucophaeata appears to be more tolerant to high temperature stress.     

Correlative changes in metabolism and DNA damage in turbot (Scophthalmus maximus) exposed to benzo[a]pyrene

Juvenile turbot (Scophthalmus maximus) were injected intraperitoneally with either corn oil or 5 mg/kg benzo[a]pyrene (BaP) dissolved in corn oil and sampled I and 3 days after injection. After 1 day, no elevation of 7-ethoxyresorufin O-deethylase (EROD) activity was observed, however bile metabolites (BaP-7,8 dihydrodiol representing 70% of the total metabolites) and a single hepatic DNA adduct spot (0.47 adducts/10(8) nucleotides) identified by 32P-postlabelling were formed. No BaP metabolites or DNA adducts were observed in either control or carrier control fish. Fish sampled after 3 days reported 5-fold higher (P < 0.05) levels of EROD activity, a shift in the bile metabolite profile towards BaP phenol formation (1OH and 30H BaP comprising up to 60% of total metabolites detected) and the formation of two adduct spots (0.86 and 0.71 adducts/10(8) nucleotides). These results show that BaP can be metabolised and form hydrophobic DNA adducts in turbot without EROD elevation. Following EROD elevation, a shift in the profile of both BaP metabolites and BaP metabolite-DNA interactions occurs indicative of other oxidative processes.     

Size of flatfish larvae at transformation, functional demands and historical constraints

At hatching the larvae of flatfish closely resemble the bilateral symmetric larvae of other teleosts, especially perciforms. Literature data show that transformation to asymmetric benthic juveniles normally occurs at body lengths between 10 and 25 mm. Unexpectedly, minimal size at its completion (including eye migration) can be 4.1 mm SL and maximal size is over 72 mm. In this paper we consider the functional requirements for a successful switch from a symmetric pelagic larva to a typical asymmetric juvenile benthic flatfish partly based on evidence from other teleosts. The unfavourable period of eye migration and transition to a benthic habitat requires some food reserves and rewiring and/or recalibration of vision and gravity-associated structures utilised previously by the still symmetric larvae for e.g. food detection. Binocular fixation of the prey probably occurs in that stage. Critical or sensitive periods occurring during development of fish larvae suggest that a completely functional symmetric stage of development must precede transformation. The normal size range in flatfish larvae at transformation seems to confirm our considerations. Recent data on temperature effects during development provide an explanation for metamorphosis at the minimal size. Some evidence for paedomorphic heterochrony in flatfish larvae is presented.     

A correcting “prism” for underwater viewpoints

The angular field observable in water by an observer in air depends on the configuration of the air-water interface. When the interface is a plane, the absolute limit to observable field is about from the normal to the interface. A practical limit, because of lateral chromatic aberration is considerably less, approximately 30°. A conventionally used configuration in research submersibles, is a polymethyl methacrylate port with inner and outer surfaces parallel. This has the same optical limitations as the plane air-water interface. It is shown that if the inner and outer surfaces are not required to be parallel to each other, there are solutions which permit extending the observable field to nearly a full hemisphere with acceptably small distortion and lateral chromatic aberration.