Artificial light at night: implications for early life stages development in four temperate freshwater fish species

Flora and fauna have both evolved under a natural cycle of light and dark. But especially in urban areas, the night is now increasingly disturbed by artificial light. Many traits and behaviours in fish are triggered by a circadian clock, for example hatching and swim bladder inflation, which predominantly take place at dusk or night. As lighting becomes brighter and extends farther into rural areas, the distinction between day and night becomes increasingly blurred. Therefore, the loss of diurnal trigger by artificial light at night was hypothesized having deleterious effects on these traits and impact fish reproduction. To assess these effects, eggs of four native freshwater fishes, Eurasian perch Perca fluviatilis, roach Rutilus rutilus, bleak Alburnus alburnus and chub Leuciscus cephalus, were incubated under two different light conditions: a photoperiod of 14 h light:10 h darkness (LD) and continuous illumination (LL). The time to hatch and swim bladder inflation was recorded. The species showed inconsistent reactions to the light treatments. In roach and bleak, the time to 50% hatch was longer in LL, whereas continuous lighting had an accelerating effect in chub. Incubation in LL elongated the hatching period in perch and roach and, in perch, the onset of darkness seemed to trigger hatching. The swim bladder inflation was significantly promoted by continuous light in chub and bleak but was not affected in roach. In conclusion, nocturnal artificial illumination could have an effect on hatching and initial swim bladder filling by masking the day–night-change and thereby diminish the trigger effect. However, the reactions were species specific and the increase in variation indicated a lack of diurnal triggering, whilst a general deleterious effect of artificial light at night has not been identified on early life stages.     

广东房地产业发展现状与前景分析

房地产开发热潮令理论界，新闻界和广大民众所关注，本文试图对广东省房地产发展的现状、特点及其主要问题作全面的分析，并提出解决问题的对策和建议，以及对广东省房地产业的发展前景作尝试性的探讨。     

GM4型磁通门磁力仪性能检验方法

目前,磁通门磁力仪广泛应用于观测地球磁场相对变化的仪器。相对于应用,仪器的性能测试不为人们所熟悉。本文针对GM4型磁通门磁力仪的噪声特性,频率特性,线性度,温度特性等关键性能指标的测试依据、测试方法及流程给予详细的阐述。     

Simulating the moderating effect of a lake on downwind temperatures

A steady-state, two-dimensional numerical model is used to simulate air temperatures and humidity downwind of a lake at night. Thermal effects of the lake were modelled for the case of moderate and low surface winds under the cold-air advective conditions that occur following the passage of a cold front. Surface temperatures were found to be in good agreement with observations. A comparison of model results with thermal imagery indicated the model successfully predicts the downwind distance for which thermal effects due to the lake are significant.     

Automated mapping of rock slope geometry,kinematics and stability with RSS-GIS

A GIS-implemented, deterministic approach for the automated spatial evaluation of geometrical and kinematical properties of rock slope terrains is presented. Based on spatially distributed directional information on planar geological fabrics and DEM-derived topographic attribute data, the internal geometry of rock slopes can be characterized on a grid cell basis. For such computations, different approaches for the analysis and regionalization of available structural directional information applicable in specific tectonic settings are demonstrated and implemented in a GIS environment. Simple kinematical testing procedures based on feasibility criteria can be conducted on a pixel basis to determine which failure mechanisms are likely to occur at particular terrain locations. In combination with hydraulic and strength data on geological discontinuities, scenario-based rock slope stability evaluations can be performed. For conceptual investigations on rock slope failure processes, a GIS-based specification tool for a 2-D distinct element code (UDEC) was designed to operate with the GIS-encoded spatially distributed rock slope data. The concepts of the proposed methodology for rock slope hazard assessments are demonstrated at three different test sites in Germany.     

Observations of estuarine circulation and solitary internal waves in a highly energetic tidal channel

Despite vigorous tidal and wind mixing, observations in an estuarine tidal inlet in the Wadden Sea show that during part of the tidal cycle, vertical stratification and internal waves may still develop. Acoustic Doppler current profiler (ADCP) and conductivity, temperature, depth observations, collected over the past 6 years at 13 h anchor stations (ASs), reveal that these occur especially during slack tide, when there is little wind and large freshwater discharge from nearby Lake IJssel. Measurements with a moored ADCP show that in the same tidal phase, strong cross-channel circulation develops, which may suddenly reverse circulation sense due to passing density fronts. In the vertically stratified phase that follows after the front passage, propagating mode-one solitary internal waves are observed. These are resonantly generated during decelerating tidal ebb currents when the (shear) flow passes a transcritical regime (Froude number equal to 1). A combination of photographs (including one from the International Space Station), bathymetric data, and ASs data leads to the discovery of yet another source of internal waves in this area, produced during slackening tide by propagating lee waves that develop over a deep trench. We suggest that both the cross-channel circulation as well as the (solitary) internal waves may locally be of importance for the (re)distribution and transport of sediments and nutrients and may influence tidally averaged transports.     

Single Nucleotide Polymorphism of FSHβ Gene Associated with Reproductive Traits in Japanese Flounder (Paralichthys olivaceus)

Follicle stimulating hormone β (FSHβ) of Japanese flounder (Paralichthys olivaceus) plays a key role in the regulation of gonadal development.This study aimed to investigate molecular genetic characteristics of the FSHβ gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of FSHβ on reproductive traits in Japanese flounder.We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the FSHβ gene in 60 individuals.We identified only an SNP (T/C) in the coding region of exon3 of FSHβ.The SNP (T/C) did not lead to amino acid changes at the position 340 bp of FSHβ gene.Statistical analysis showed that the SNP was significantly associated with testosterone (T) level and gonadosomatic index (GSI) (P0.05).Individuals with genotype TC of the SNP had significantly higher serum T levels and GSI (P0.05) than that of genotype CC.Therefore, FSHβ gene could be a useful molecular marker in selection for prominent reproductive trait in Japanese Flounder.     

Comparative assessment of the relationship of satellite data with the above ground biomass of Sal trees (<Emphasis Type="Italic">Shorea robusta</Emphasis>) determined from phenologically different time periods

The Above Ground Biomass(AGB) estimates of vegetation comprise both the bole biomass determined through a volumetric equation and litter biomass collected from the ground.For mature trees,the AGB estimated in phenologically different time periods is directly affected by the litter biomass since the Diameter at Breast Height(DBH) and height(H) of such trees that are used in the estimation of bole biomass would remain unchanged over a reasonable time period.In the present study,we have determined the AGB of Sal trees(Shorea robusta) in two contrasting seasons:the peak green period in October being devoid of lit-ter on the ground and the leaf shedding period in February with abundant amount of litter present on the ground.Estimation of AGB for the month of February included the litter biomass.In contrast,the AGB for October represented only the bole biomass.AGB was estimated for ten different plots selected in the study area.The AGB estimated from ten sampling plots for each time period was re-gressed with the individual tree parameters such as the average DBH and height of trees measured from the corresponding plots.The regression analysis exhibited a significantly stronger relationship between the AGB and DBH for the month of October as compared to February.Furthermore,the correlation between the remotely sensed derived data and AGB was also found to be significantly higher for the month of October than February.This observation indicates that inclusion of the litter biomass in AGB will tend to decrease the re-gression relationship between AGB and DBH and also between the remotely sensed data and AGB.Therefore,these conclusions invite careful consideration while estimating AGB from satellite data in phenologically different time periods.     

Resolving mechanisms of toxicity while pursuing ecotoxicological relevance?

In this age of modern biology, aquatic toxicological research has pursued mechanisms of action of toxicants. This has provided potential tools for ecotoxicologic investigations. However, problems of biocomplexity and issues at higher levels of biological organization remain a challenge. In the 1980s and 1990s and continuing to a lesser extent today, organisms residing in highly contaminated field sites or exposed in the laboratory to calibrated concentrations of individual compounds were carefully analyzed for their responses to priority pollutants. Correlation of biochemical and structural analyses in cultured cells and tissues, as well as the in vivo exposures led to the production and application of biomarkers of exposure and effect and to our awareness of genotoxicity and its chronic manifestations, such as neoplasms, in wild fishes. To gain acceptance of these findings in the greater environmental toxicology community, “validation of the model” versus other, better-established often rodent models, was necessary and became a major focus. Resultant biomarkers were applied to heavily contaminated and reference field sites as part of effects assessment and with investigations following large-scale disasters such as oil spills or industrial accidents.

Over the past 15 years, in the laboratory, small aquarium fish models such as medaka (Oryzias latipes), zebrafish (Danio rerio), platyfish (Xiphophorus species), fathead minnow (Pimephales promelas), and sheepshead minnow (Cyprinodon variegatus) were increasingly used establishing mechanisms of toxicants. Today, the same organisms provide reliable information at higher levels of biological organization relevant to ecotoxicology. We review studies resolving mechanisms of toxicity and discuss ways to address biocomplexity, mixtures of contaminants, and the need to relate individual level responses to populations and communities.