海带雌性原生质体的分离与培养

于1993年1月 - 1993年4月，为了制备遗传性纯一的基因工程受体，用酶法从海带雌配子体单性生殖系分离雌性单倍体原生质体。从皱纹盘鲍消化腺及消化器官提取出鲍酶，在25°C下、1％（W／V）的鲍酶与2％（W／V）的纤维素酶（Onozuka R－10）合用能有效解离单细胞和原生质体。海带雌性原生质体经2d培养再生了细胞壁。     

Static Analysis of Collision Strength of Offshore Platform

In this paper a numerical analysis method combining FEM incemental technique with limit analysis concept is proposed for the study of the static strength of offshore platform in collision. Large deformation and plasticity are accounted for and the limit yield surface expressed by generalized stress for a tubular section is derived. The modified stiffness matrix of space beam element is formulated by Plastic Node Method. The buckling behavior of beam columns can also be taken into account. It can trace the generation of plastic hinges during loading and finally the ultimate strength of offshore platform against collision is obtained.     

Optimal Design for Main Dimensions of a 75000t Floating Production and Storage Vessel

The basis, process and results of the demonstration of the main dimensions of a 75000t floating production and storage vessel are discussed in this paper. A simple but reliable orthogonal design method is applied in the main dimension optimization. The ideas of gradual approximation and feedback from various aspects are put into effect. During the demonstration, in order to make the model tally with the actual situation, the draft design is closely related to the computational analysis, so that the demonstration model can be verified at any time; the handling of the overall system is closely related to the research of each item, which is beneficial not only to the mastery of various regularities, but also to the balance of decisions. Finally, according to the computational results and the regularities obtained from analysis, the main dimensions are determined.     

Elasto-Plastic Analysis of Tubular Joints of Offshore Platforms by Finite Element Method

The plastic node method is reformulated by the variational principle and is applied to elasto-plastic finite element analysis of tubular joints, eventually including the effect of internal and external gussets, stiffener rings, etc., if necessary. Four different joints are studied here in detail for the elasto-plastic behavior, the strain at the hot spot, the strain concentration factor around the intersection line, and the propagation of the plastic region with loading up to collapse in order to determine the ultimate strength, safety factor, and development of the plastic field. The present results are in good agreement with the experimental results.     

PROPAGATION AND TRANSFORMATION OF NON-LINEAR WAVES ON UNIFORMLY SLOPING BEACHES Ⅰ. A SOLUTION FOR NON-LINEAR PROGRESSING WAVES

Two-dimensional non-linear hydrodynamical equations are solved by using perturbation method and treating slopping beaches as bottom boundary conditions so that a kind of solution for nonlinear progressing waves is obtained. The first order of approximation is the same potential function as used by Biesel, and the second order is calculated numerically. Based on the solution, wave characteristics before breaking, especially the wave set-down, are discussed. It turns out that for the whole course of waves propagating from deep to shallow waters the theory proposed in this paper has a wider valid range of application than others.     

中国沿岸水域养殖贝类及其养殖笼网污损苔虫7新种

拟小孔苔虫属Microporella和斑孔苔虫属Fenestrulina均隶于唇口目Cheilostomida有囊亚目Ascophorina皮壳次目 Lepraliomorpha裂孔超科Schizoporelloidea拟小孔苔虫科Microporellidae。这两个属的主要区别在于：拟小孔苔虫属具有鸟头体，而斑孔苔虫属无鸟头体。我们在研究中国沿岸水域养殖贝类及其养殖笼网的污损苔虫时发现，中国一些学者以前所报道的“纤毛拟小孔苔虫”并非 Pallas(1766)所鉴定的种，而是包括不同于纤毛拟小孔苔虫Microporella ciliata Pallas，1766的5个不同的独立种，即本文所描述的拟小孔苔虫属的5个新种：空穴拟小孔苔虫 Microporella vacuatus sp.nov.、小筛网拟小孔苔虫Microporella cribellata sp.nov.、无齿拟小孔苔虫Microporella inermis sp.nov.、异北方拟小孔苔虫 Microporella antiborealis sp.nov.和项链拟小孔苔虫 Microporella monilifera sp.nov.。这5个新种与纤毛拟小孔苔虫的主要区别是:5新种的口刺为2~6根，除无齿拟小孔苔虫的口上卵胞仅具一列边缘孔外，其他4新种卵胞前表面还有分散的穿孔；而纤毛拟小孔苔虫的口刺为6根，口上卵胞细颗粒状，无穿孔。研究中我们还发现前人所定名为“马氏斑孔苔虫”的种并非 Audouin(1826)所描述的种，而是4种不同于马氏斑孔苔虫 Fenestrulina mallusii Audouin，1826的独立种，其中两种不属于污损苔虫的范畴，另外的两种即本文所描述的斑孔苔虫属的两新种，即中华斑孔苔虫Fenestrulina Sinica sp.nov.和东方斑孔苔虫Fenestrulina orientalis sp.nov.。这两个新种与马氏斑孔苔虫的主要区别在于：两新种口刺均为4~6根，受孕个虫有2根裸露的口刺；而马氏斑孔苔虫的口刺为2~3根，受孕个虫无裸露的口刺。     

三疣梭子蟹全人工工厂化育苗技术

2000年笔者在日照市水产研究所利用1450m^3水进行梭子蟹全人工工厂化育苗生产，共出Ⅱ-Ⅲ期幼蟹512．7万只，创造了出苗量为3500只／m^3，总产值为150多万元的直接经济效益，同时对幼体培育的密度进行了初步试验，结果表明：从Z1至M期幼体培育密度10－15万／m^3为宜，M期以后密度以0．8－1．5万／m^3为宜。     

高分辨率遥感技术在厦门海湾生态环境调查中的应用

对于局部区域研究，高分辨率卫星遥感及其影像的智能化处理技术是获取信息的新手段．本文以IKONOS遥感影像在厦门海湾的应用为例，探讨高分辨率遥感卫星影像应用于海湾生态环境调查与分析的应用技术．通过分析高分辨率影像地物特征，提出高分辨率遥感影像的重要处理技术：采用不同缩放尺度进行分类和利用空间特征及纹理结构进行专题信息提取。     

桶形基础平台三维有限元稳定性分析

以我国第一座桶形基础采油平台工程实例为原型，采用三维有限元计算方法对桶形基础稳定性分析。平台在波浪、风等荷载作用下，其稳定性不仅与平台的整体结构有关，而且与桶形基础及其周围土体相互作用相关。在计算中，利用薄层摩擦单元模拟土与结构间的相互作用，取得了很好的效果。分析结果表明桶形基础采油平台在设计条件下运行是较为稳定的。     

唐山市滨海湿地动态演变特征及其机制分析

利用历史资料和Landsat TM卫星遥感图像,研究了唐山市滨海湿地自建国以来的演变特征,着重对1987~2004年陡河———滦河之间的滨海平原湿地类型进行了识别,绘制了不同时期的景观面解译图。利用Mapinfo制图软件计算出两个时期的面积(比例)转化矩阵,显示该区滨海湿地破碎化加重,其动态演变特征呈明显退化趋势,进而提出引起其退化的原因和发展趋势预测。