Salinity-induced survival strategy of Vibrio cholerae associated with copepods in Cochin backwaters

The occurrence of Vibrio cholerae in water, sediment and copepods was studied over a wide range of salinity using conventional and polymerase chain reaction (PCR) techniques in the Cochin backwaters. V. cholerae occurred either as culturable or non-culturable form in this tropical estuary. During the pre monsoon period when high salinity prevailed (35.5psu) V. cholerae was associated with copepods only in non-culturable form, but with the onset of monsoon when lower salinity prevailed, copepod-associated-V. cholerae reverted back to culturable form. The optimum salinity range for culturability was found to be 15-20psu. This points to the fact that salinity is a major factor that which influences V. cholerae's culturability and its association with copepods in Cochin backwaters.     

Assessment of the natural resources of groundwater by using balance-hydrodynamic models

The specific features of formulation and implementation of balance-hydrodynamic simulation during different-scale studies of natural resources of groundwater. Are an example of detailed estimate of the resources and balance of groundwater in the Vologda River basin is given. It is noted that the use of balance-hydrodynamic models, in addition to an increase in the general reliability of estimates, allows the calculation of normal annual characteristics of groundwater resources with different occurrence (exceedance probability), which is impracticable when hydrodynamic methods in other formulations are used.     

2,4,6-trinitrotoluene transformation by a tropical marine yeast, Yarrowia lipolytica NCIM 3589

Yarrowia lipolytica NCIM 3589, a tropical marine degrader of hydrocarbons and triglycerides transformed 2,4,6-trinitrotoluene (TNT) very efficiently. Though this yeast could not utilize TNT as the sole carbon or nitrogen source, it was capable of reducing the nitro groups in TNT to aminodinitrotoluene (ADNT). In a complete medium containing glucose and ammonium sulphate as the available carbon and nitrogen sources respectively, the culture was able to completely transform 1 mM (227 ppm) of TNT under such conditions. A dual pathway was found to be functional, one of which resulted in the formation of the hydride-Meisenheimer complex (H(-)TNT) as a transiently accumulating metabolite that was subsequently denitrated to 2,4-dinitrotoluene (2,4-DNT), whereas the other pathway resulted in the formation of amino derivatives. The presence of increasing amounts of reducing equivalents in the form of glucose promoted better growth and the nitroreductases of this yeast to reduce the aromatic ring to 2,4-DNT although, the reduction of the nitro groups to amino groups was the major functional pathway. The ability of this tropical marine yeast to transform TNT into products such as 2,4-DNT which in turn could be metabolized by other microbes has implications in the use of this yeast for bioremediation of TNT polluted marine environments.     

Behavior of the broad Hα component in the Seyfert galaxy NGC 4151 in 1976–2003

Variations in the flux and profile of the broad Hα component in the Seyfert galaxy NGC 4151 are analyzed based on spectral observations acquired from 1976 to spring 2003. The procedure used to distinguish components is described. There is a strong correlation between the flux of the broad Hα component and the U flux of the galactic nucleus. There was an appreciable increase in the intensity of the broad Hα in 1990–2000, with the flux reaching a maximum in 1996. The intensity and shape of an emission feature that appeared in the red wing of the broad Hα component at the end of 2002 are estimated.     

Distribution and accumulation rate of ore elements in Holocene and Late Glacial Sediments of the Deryugin Basin,Sea of Okhotsk

Results of the study of contents and accumulation rates of Fe, Mn, and a number of trace elements in Upper Quaternary sediments of the Deryugin Basin are presented. Maps of the average contents and accumulation rates of excess Fe, Mn, Zn, Ba, Ni, Pb, Cu, and Mo in sediments of the first oxygen isotope stage (OIS) have been plotted. Anomalous contents and accumulation rates are confined to peripheral zones of the Deryugin sedimentary basin and large fracture zones. Different mechanisms of the influence of fluid-dynamic processes on the rate of hydrogenic and biogenic accumulation of ore elements are assumed.Translated from Litologiya i Poleznye Iskopaemye, No. 2, 2005, pp. 115–132.Original Russian Text Copyright © 2005 by Astakhov, Gorbarenko, Bakhareva, Gretskaya, Sattarova.     

A Simple Model for the Movement of Fire Smoke in a Confined Tunnel

Fires in tunnels are unfortunately frequent occurrences often with tragic outcomes. A recent example is the fire on the funicular train at the ski resort in Kaprun (Austria), which caused nearly 160 deaths. Design engineers and risk analysts require knowledge of the fluid dynamics of the fire and smoke movement to answer questions such as how much oxygen can access and feed the fire, and what concentration of smoke will the people be exposed to. As an example in the Austrian accident the geometry was a long tunnel with fire doors closed at one end, and with a fire initiated near the closed (lower) end. The hot smoke from the fire is a source of buoyancy; the smoke reaches the ceiling of the tunnel, and then develops along the ceiling as a wall-bounded plume. The motion of the smoke is driven by a buoyancy force, but at the same time, mechanisms of turbulent heat and mass transfer act as a brake to this motion. In this paper we present how a generic model describing a semi-enclosed buoyancy-driven flow can be interpreted and used in the modelling of fire smoke movement in a confined tunnel. A consideration of the net pollutant volume flux through the tunnel leads to predictions for the variation of concentrations along the tunnel. The smoke concentrations near the fire smoke source scale linearly with the length of the tunnel, with higher concentrations at the lower section of the tunnel, as could be expected. Similarly the concentration of oxygen making its way through to the fire source decreases linearly with the length of the tunnel. A lower bound estimate of the smoke residence time can be obtained based on smoke concentration predictions from the model.