This study on the effects of ultrasonic treatment on female gametophytes ofLaminaria japonica showed that:
1.
Ultrasonic treatment had shortening effect on filaments of female gametophytes. Within certain period of time, the average length of filamentous female gametophytes was shortened.
2.
Ultrasonic treatment had emptying effect on cells. The number of empty cells increased with time of treatment. Ultrasonic treatment had harmful effect on cells.
3.
Ultrasonic treatment could break down cell walls. The combination of frequency of 20 kHz, output of 15 W, 40 s and 60 s of treatment was best for this purpose. After ultrasonic treatment, the regeneration of female gametophytes into sporophytes was effected. Female ganetophytes could not recover after too long period of treatment.
Due to rapid economic growth in the Pingtung Plain of Taiwan, the use of groundwater resources has changed dramatically.
Over-pumping of the groundwater reservoir, which lowers hydraulic heads in the aquifers, is not only affecting the coastal
area negatively but has serious consequences for agriculture throughout the plain. In order to determine the safe yield of
the aquifer underlying the plain, a reliable estimate of groundwater recharge is desirable. In the present study, for the
first time, the chloride mass-balance method is adopted to estimate groundwater recharge in the plain. Four sites in the central
part were chosen to facilitate the estimations using the ion-chromatograph and Thiessen polygon-weighting methods. Based on
the measured and calculated results, in all sites, including the mountain and river boundaries, recharge to the groundwater
is probably 15% of the annual rainfall, excluding recharge from additional irrigation water. This information can improve
the accuracy of future groundwater-simulation and management models in the plain.
Received, April 1996 Revised, March 1997, November 1997 Accepted, March 1998 相似文献