Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.     

Influence of air masses on particle number concentration and size distribution at Mt. Waliguan, Qinghai Province, China

Particle size distribution of 12–500 nm was measured at Mt. Waliguan, China Global Atmosphere Watch Baseline Observatory, from Aug. in 2005 to May in 2007. 72-hr back-trajectories at 100-m arrival height above ground level for the same period were calculated at 6:00, 12:00, and 21:00 (Beijing Time) for each day using the Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT-4) model developed by NOAA/ARL. It was found that air mass sources significantly impact particle number concentration and size distribution at Mt. Waliguan. Cluster analysis of back-trajectories show that higher Aitken mode particle number concentration was observed when air masses came from or passed by the northeastern section of Mt. Waliguan, with short trajectory length. High number concentration of nucleation mode was associated with air masses from clean regions, with long trajectory length     

Combined effects of light intensity and NH^＋4-enrichment on growth,pigmentation, and photosynthetic performance of Ulva prolifera （Chlorophyta）

The aim of this study was to investigate the effects of light intensity and enhanced nitrogen supply on the growth and photosynthesis of the green-tide macroalga, Ulva prolifera. Thalli of U. prolifera were grown in natural or NH 4 +-enriched seawater under two different light intensities for 7 days, and then the growth rate, pigmentation, and photosynthetic performance of the thalli were evaluated. The results show that the relative growth rate(RGR) was markedly higher under the high light level than under the low light level. Enrichment with NH 4 + enhanced the RGR under high light intensity, but did not affect RGR under low light intensity. In low light conditions, NH 4 +-enrichment resulted in a marked decrease in the maximal photosynthetic rate( P m) and the maximum carbon fixation rate( V max), but it did not affect the half saturation constant for carbon( K 0.5) or the ratio of V max to K 0.5, which reflects the carbon acquisition efficiency. In high light conditions, P m, K 0.5, and the dark respiration rate( R d) increased under NH 4 + enrichment, but V max and the V max / K 0.5 ratio decreased. Regardless of the light intensity, NH 4 +-enrichment did not affect the apparent photosynthetic efficiency( α), which refl ects the ability of the alga to use light energy at low light levels. Under both low and high light intensities, the chlorophyll a(Chl a), chlorophyll b(Chl b), and carotenoids(Car) contents in thalli were higher in NH 4 +-enriched than in natural seawater, except that there was a decrease in the Chl b content of thalli in NH 4 +-enriched seawater under low light intensity. Therefore, NH 4 + enrichment improved the growth and photosynthetic performance of U. prolifera under high light intensity, but not under low light intensity. We discuss the possible mechanisms underlying these physiological responses.     

Hadoop环境下基于SparkSQL海量自动站数据查询统计初探

在Hadoop分布式计算和存储架构下,自定义ETL数据清洗规则将海量自动站小时单站文件按所属年和站号合并为大文件流转存储至HDFS中,并运用SparkSQL并行计算框架进行统计处理生成常用气象要素日统计值。结果表明,数据处理和获取时效较关系型数据库方式有显著提升。采用SparkSQL并行计算框架对多气象要素多站点和长时间序列进行数据统计处理查询均能达到秒级别响应,并随着统计站点数的不断增加和时间跨度的延长其优势更为明显,能更高效地支撑此类气象数据服务,为海量气象数据处理从关系型数据库到大数据分布式架构的转换处理提供了新思路。     

长江口现代潮滩沉积物粒度特征及其在沉积相识别中的应用

本研究通过对长江口现代潮滩6个柱状样沉积物的粒度精细分析,试图提取研究区潮滩沉积相识别的粒度敏感指标,并通过对长江三角洲南部平原一个全新世钻孔(SL67孔)潮滩沉积物的粒度研究,检验现代沉积粒度敏感指标在全新世钻孔潮滩沉积相识别中的应用。研究显示,长江口现代高潮滩和中潮滩的黏性颗粒(8μm)和粗粉砂(32~63μm)含量差异显著,可以成为区分高潮滩和中潮滩的敏感组分;在开敞型的中、低潮滩之间,砂(63μm)和细粉砂含量(8~32μm)也差异明显。长江口现代潮滩沉积物的粒度频率曲线及众数值也可以有效协助区分高潮滩、中潮滩和低潮滩沉积物。利用上述粒度敏感组分和众数指标检验SL67孔全新世早中期在海平面波动控制下的潮滩演替过程,并揭示了7.5 cal ka BP前后的一次海平面加速上升事件。     

基于气候适宜度的夏玉米发育期模拟模型

结合前人气候适宜度的研究成果,以作物生理生态发育过程为基础,构建了夏玉米发育期预报模型。模型中分别建立了夏玉米温度、降水、日照时数适宜度函数,并结合河南省19个农业气象试验站的夏玉米发育期资料,运用通径分析法确定各个生育期温度、降水和日照的影响权重系数,计算出综合适宜度,用来预测夏玉米生育期。结果表明,模型能够较好地预测各个发育期(出苗、七叶、拔节、抽雄、乳熟和成熟)。建模资料的模拟值与观测值比较的均方根误差分别为1.5、3.1、3.4、2.9、4.0、4.5 d。运用独立资料对模型所作预测值的均方根误差在1.0~4.6 d之间。     

大地电磁自适应正则化反演算法

针对大地电磁正则化反演中正则化因子的选取困难问题提出了自适应正则化反演算法（Adaptive Regularized Inversion Algorithm, ARIA）. 在该算法中, ①提出了一种新的数据方差处理方法：数据方差规范化,使得数据方差的大小只对数据的拟合发生影响,不对数据目标函数和模型约束目标函数的权重产生影响,从而减少了正则化因子取值的影响因素;②提出了粗糙度核矩阵的概念,并给出了由基本结构插值基函数计算粗糙度核矩阵的公式,使得模型目标函数的构建更为简便、直接;③根据数据目标函数、模型约束目标函数和正则化因子之间的关系,提出了两种正则化因子自适应调节方法. 本文详细阐述了最平缓模型约束下的大地电磁一维连续介质反演的ARIA实现,以几个算例的分析比较来说明ARIA的有效性.     

江西省温室气体监测及关键领域碳排放核算工作进展

简要概括了“十三五”规划实施以来,江西省开展温室气体浓度监测及关键领域排放核算相关工作及研究进展。1) 阐述了江西省温室气体观测站网建设现状,分析了各监测站浓度变化特征,对监测数据质量进行了评估,规范了业务服务产品;2) 基于景德镇站温室气体观测数据,开展了大气CO2和CH4浓度变化及其源汇特征相关研究,摸清其区域输送影响及源汇特征;3) 通过对卫星资料的分析研究,揭示了江西省大气温室气体浓度时空分布特征;4) 通过连续多年重点企业碳排放核查,促进了碳排放权交易市场的建立;5) 完成江西全省及11个设区市农业温室气体清单的编制,掌握了农业温室气体排放动态变化,编制发布了相关技术规范。江西省温室气体监测起步较晚,相较于山西、广东、江苏等省仍存在一定差距,亟待配备充足的资金和人员,规范台站观测业务运行,加强数据质控和技术队伍建设;此外,有必要加强各类排放过程排放因子本地测算,进一步规范碳核查工作流程,为江西省生态文明建设、绿色低碳发展及应对气候变化提供支撑。     

周期浅析

本文分析了滑动平均的数据处理方法对周期分析结果产生的影响。提出了过程变量同号积分的数据处理方法,并对亚洲西风指数序列进行了试验分析。分析结果表明,周期的长短与振幅的大小有密切关系,亚洲逐日西风指数的周期振荡具有明显的季节特征。     

无拉伸动校正技术在近海工程地震勘探中的应用

动校正是地震资料处理的关键内容之一,直接关系到地震资料处理结果的精度.在浅层和大偏移距情况下,常规动校正使波形发生拉伸畸变,波形拉长、频带向低频方向移动,进而影响叠加效果.通常地震资料处理中大都采用切除的方法克服动校正拉伸畸变的影响,这对目的层较深时是可取的.以工程地质调查为目的的海洋地震勘探旨在了解海底之下较浅地层深度范围内的地质信息,一般勘探区水深较浅,数据叠加道数较少,如果仍然采取切除处理,势必严重影响资料的分辨率和准确度.本文阐述了一种无拉伸动校正方法,能较好地解决动校正后的波形畸变问题,理论模型和实际资料的处理结果表明该方法在近海浅层工程地震勘探中是行之有效的,有助于提高速度分析的精度和地震资料的分辨率.