Comparative assessment of nitrogen fixation rate by 15N2 tracer assays in the South China Sea

Nitrogen fixation is one of the most important sources of new nitrogen in the ocean and thus profoundly affects the nitrogen and carbon biogeochemical processes. The distribution, controlling factors, and flux of N₂ fixation in the global ocean remain uncertain, partly because of the lack of methodological uniformity. The 15N₂ tracer assay (the original bubble method → the 15N₂-enriched seawater method → the modified bubble method) is the mainstream method for field measurements of N₂ fixation rates (NFRs), among which the original bubble method is the most frequently used. However, accumulating evidence has suggested an underestimation of NFRs when using this method. To improve the availability of previous data, we compared NFRs measured by three 15N₂ tracer assays in the South China Sea. Our results indicate that the relationship between NFRs measured by the original bubble method and the 15N₂-enriched seawater method varies obviously with area and season, which may be influenced by incubation time, diazotrophic composition, and environmental factors. In comparison, the relationship between NFRs measured by the original bubble method and the modified bubble method is more stable, indicating that the N₂ fixation rates based on the original bubble methods may be underestimated by approximately 50%. Based on this result, we revised the flux of N₂ fixation in the South China Sea to 40 mmol/(m2·a). Our results improve the availability and comparability of literature NFR data in the South China Sea. The comparison of the 15N₂ tracer assay for NFRs measurements on a larger scale is urgently necessary over the global ocean for a more robust understanding of the role of N₂ fixation in the marine nitrogen cycle.     

Aliphatic biomarker signatures of early Oligocene-early Miocene source rocks in the central Qiongdongnan Basin: Source analyses of organic matter

The geochemical signatures of fifty-four rock samples and three supplementary drill stem test(DST) oils from the Yacheng-Sanya formations in the central Qiongdongnan Basin(CQB) were analysed. Reconstruction of the early Oligocene-early Miocene(36–16 Ma) palaeovegetation and source analyses of organic matter(OM) were conducted using aliphatic biomarkers in ancient sediments and DST oils. Both the interpreted aquatic and terrigenous OM contributed to the CQB source rocks(SRs) but had varying relat...     

南海西北部水域斑节对虾资源的调查研究

1989－1991年对南海西北部水域进行斑节对虾Penaeusmonodon资源专项调查。结果表明,斑节对虾的密集分布区在海南岛东部和南部海区,其现存资源量为82．6万尾,周年有体长30mm以下的幼虾出现。主要产卵期在9－12月。产卵场在海南岛东部和南部水深30-60m的海区。     

商业平板式超滤膜包完整性检验：对海洋胶体生物地球化学研究的启示

The performance and integrity of a cassette cross-flow ultrafilter(Pellicon 2, Millipore) are examined with a suite of macromolecules of different molecular masses. The retention coefficient during the cross-flow ultrafiltration experiments increases with increasing molecular mass and reaches 90% with 10 kDa dextran in both milli-Q water and ultrafiltered seawater media. Based on a 90% retention coefficient, the molecular mass cut-off for the ultrafiltration membrane is defined at 10 kDa, which is ten times(1 kDa) that rated by the manufacturer. To further validate the accuracy of the laboratory calibration, the samples from the lower Zhujiang River and the Jiulong River Estuary are ultrafiltered with the cassette ultrafiltration membrane and the colloidal organic carbon abundances in these samples are quantified with the ultrafiltration permeation model based on time series permeation subsamples. The colloidal organic carbon abundances are 5.8%–21.1% in the Jiulong River Estuary and 5.6%–11.0% in the lower Zhujiang River. These are consistent with the reported values for both estuaries as well as with the colloidal organic carbon abundances in marine environments over the coastal and open oceans with 10 kDa cut-off membranes. Therefore, these field data support the laboratory calibration result and indicate the validity of the experimental and quantification procedure adopted. The discrepancy between the nominal molecular mass cut-off and the actual pore size of the ultrafiltration membrane should be of great concern for research in colloidal and nanoparticle biogeochemistry. Careful examination of the membrane integrity should be taken during ultrafiltration experiments in order to avoid misleading molecular mass cut-off information.     

海洋细菌脂肪酸的气相色谱分析

以副溶血性弧菌VP-X-3为试验对象,利用气相色谱技术分析了其细胞脂肪酸的组成和含量,以色谱峰的数量、色谱峰高及脂肪酸含量作为评价指标,探讨了不同的皂化和甲酯化条件对细菌细胞脂肪酸测定结果的影响。结果表明:皂化和甲酯化条件都对细胞脂肪酸的组成和含量产生一定的影响。较低温度皂化处理利于增加色谱峰数量和色谱峰高。甲酯化温度过高容易破坏脂肪酸,尤其是长链脂肪酸和不饱和脂肪酸的结构。高温甲酯化条件下,时间越长,甲酯化效果越差。试验得到较为理想的气相色谱前处理条件:2 mol/L NaOH-甲醇溶液2 mL,70℃水浴10 min;10%(V/V)H2SO4-甲醇溶液2 mL,70℃水浴15 min。此条件下的色谱峰的数量多达29种,色谱峰高明显高于其他处理条件下的峰高。     

半滑舌鳎(Cynoglossus semilaevis)双链RNA依赖的蛋白激酶R(PKR)基因克隆和实时定量表达分析

采用RACE方法克隆了半滑舌鳎(Cynoglossus semilaevis)PKR基因(CsPKR), 获得了CsPKR全长cDNA序列为2907bp, 其中包含1959bp的开放阅读框, 100bp的5′非编码区和848bp的3′非编码区。保守结构域分析显示推导的CsPKR氨基酸在N端存在两个特异的dsRBD(dsRNA binding domain dsRBD)结构域, 在C端具有多个保守的激酶活性位点, 包括ATP结合位点和底物配体结合位点, 以及活性环结构A-loop。系统进化树分析显示鱼类、两栖类、鸟类及哺乳类的PKR具有共同的进化起源, CsPKR与牙鲆PKR的亲缘关系最为密切。荧光定量PCR结果显示, CsPKR基因在健康鱼多个组织中广泛表达, 在脑中的表达最高, 头肾中表达最低。经鳗弧菌和淋巴囊肿病毒分别感染后, CsPKR基因在免疫相关组织中呈现上调表达趋势, 其中感染鳗弧菌12h后在血液中表达量较对照组上升了9.28倍, 在感染淋巴囊肿病毒24h后, 在肝脏中的表达达到最大, 为对照组的9.97倍。以上结果暗示CsPKR基因在半滑舌鳎响应细菌和病毒免疫应答中起重要作用。     

水环境中的信息有机物与“水域生态讯息学”的提出

信息有机物(或化感物质)系指存在于自然环境,对生物物种或生物类群生存和生长有促进或抑制作用,并能通过调控生物丰度与群落结构来影响生态系统和生态环境的微痕量有机物。目前已知的信息有机物大多是分子质量小于10~4Da的醇、酚、萜、酯、多肽等小分子物质,它们随水体迁移扩散,通过控制酶活性或光合系统等途径影响目标生物生存生长,作用强度受微生物分解和营养水平等多种因素影响。水体中信息有机物一般是通过有机溶剂萃取或固相萃取富集后用色质联用鉴定结构并测定含量。本文系统总结了近年来水环境中信息有机物及其检测方法的主要研究进展,归纳总结了高效灵敏的水体信息有机物检测方法,阐述了水环境中信息有机物种类、结构、作用机制及其对水域生态学的意义,在此基础上,提出了"水域生态讯息学"的新概念,诠释了"水域生态讯息学"的内涵,这对于拓展生态学研究内容,揭示水生生物学发生机制,持续利用水生生物资源具有重要的科学意义和实际价值。     

FLU-net：用于表征页岩储层微观孔隙的深度全卷积网络

页岩孔隙是页岩气储集的主要空间,孔隙的形状、大小、连通性与发育程度很大程度上决定了页岩储集层的储集性能,因此,页岩气开采首先需要对其孔隙有充分的认识。基于阈值分割法获取页岩孔隙结构参数是目前页岩微观结构表征的一种重要手段,但是受扫描电镜图像灰度分布差异的影响,该方法需要逐一修改图像的最佳分割阈值以达到最好的孔隙分割效果,且阈值分割方法无法直接划分孔隙类别,这给后续的页岩微观结构定量表征带来了麻烦。为了实现页岩孔隙的智能识别和分类,笔者设计基于像素级语义分割的深度全卷积神经网络FLU-net,对页岩孔隙识别并分类为有机孔、无机孔（粒内孔、粒间孔）及裂缝,并结合孔隙尺度分类统计方法,分析不同类型孔隙发育数量、孔径大小、孔隙度等参数,实现页岩储层微观孔隙结构的自动化定量表征。以重庆渝西区块足201井区和四川盆地威远地区威204井区的页岩扫描电镜图像为研究对象,在对1 600幅页岩扫描电镜图像原始数据进行人工标注并划分数据集后,使用FLU-net进行孔隙识别,结果表明,本方法具有较高的准确率,同时自动化程度和泛化能力均高于传统预测方法。因此,扫描电镜与基于深度学习的语义分割模型结合是定量研究页岩微观结构表征的有效手段。     

港口及临港工业围填海规模综合评价研究

针对港口及临港工业围填海的特点,运用多目标决策理论与方法,构建了港口及临港工业围填海规模评价指标体系,包括现状评定和综合影响两类评价因子,包含自然条件、社会经济条件、资源环境影响、效益评估四类评价要素,共计15个评价指标。并根据成本型、效益型和固定型指标的特点提出了各自的标准化方法,采用主客观综合赋权法确定评价因子的权重,采用线性加权和法计算港口及临港工业围填海规模方案的综合得分,以此为依据确定围填海规模的适宜程度和适宜等级。以江苏辐射沙洲半岛式浅滩腰沙为例,运用港口及临港工业围填海规模综合评价方法,确定腰沙海域最佳的港口及临港工业围填海规模为理论基面+3.0 m。提出的研究方法能够为沿海省市港口及临港工业围填海规模评价提供参考和方法借鉴,对于围填海规模决策和围填海总量控制指标的制定具有重要意义。     

海洋重力测量动态环境效应分析与补偿

针对使用小型测量船搭载摆杆型海洋重力仪获取数据质量不高的问题,在深入分析海洋环境动态效应误差特性基础上,提出了一种基于互相关分析的交叉耦合效应修正法,对高动态海洋重力测量数据实施综合误差补偿和精细处理。使用典型恶劣海况条件下的观测数据对该方法的有效性进行了验证,结果显示,重力测线成果内符合精度从原先的±9.35×10-5m/s2大幅提升到±1.43×10-5m/s2,同时使用卫星测高反演重力对精细处理结果的可靠性进行了外部检核,外符合精度也从原先的±7.73×10-5m/s2提高到±5.63×10-5m/s2。