大菱鲆幼鱼视网膜结构及不同光谱下视蛋白基因表达特征的研究

大菱鲆(Scophthalmus maximus)是中国重要的养殖鱼类之一, 其生活史经历不同的栖息地和光环境, 视觉器官结构及视觉功能也具有适应性发育特征和可塑性。本研究以不同发育阶段的大菱鲆幼鱼为对象, 探究了其视网膜结构变化、视蛋白基因的表达特征及其与光谱之间的关系。结果表明, 随着大菱鲆幼鱼的生长发育, 其视网膜的外核层逐渐变厚; 视锥视杆层的厚度变化不明显; 而内核层与神经节细胞层逐渐变薄。视紫红质基因rh1与视蛋白基因总表达量的比例上升, 由2月龄的57.35%上升到9月龄77.19%; 视锥蛋白基因与视蛋白基因总表达量的比例下降; 其中红视蛋白基因lws 由2月龄的4.49%下降至9月龄的0.13%。将7月龄的大菱鲆幼鱼用不同光谱处理75 d后, 其视蛋白基因的表达会随光谱环境的变化而发生改变。与全光谱相比, rh2b1、sws2、sws1在红、黄光下的表达量显著下降, 而rh2b1在蓝、绿光下表达显著上升、sws2在绿光下表达显著上升, 其他则变化不显著(P<0.05)。黄、绿、蓝及全光谱下rh2基因家族与视蛋白基因总表达量的比例最高, 而红光下基因rh1的表达量占比最高。大菱鲆幼鱼在不同阶段以及不同光谱处理下表现出了视蛋白基因表达的可塑性以适应不同水层的光谱环境。本研究为探究大菱鲆对光环境的适应机制及工厂化养殖光照调控技术的建立提供理论参考。     

1998年春深圳湾环节环沟藻赤潮及其发生原因的探讨

1998年4月23日至29日深圳湾发生了较大规模的环节环沟藻赤潮,面积逾200km2,细胞密度高达3.8×106 个/L。赤潮发生于大量降雨之后 ,高峰期水体盐度在20以下。在深圳河河口海域 ,虽然富营养化程度也较高 ,但由于悬浮物含量过高 ,浮游植物及环节环沟藻数量较少。赤潮期间 ,深圳湾没有发生死鱼现象 ,养殖牡蛎的大量死亡估计与赤潮无关     

鱼类生长激素的体内代谢、分泌调控和作用机制研究的进展

主要对１９８３－１９９６年间关于鱼类生长激素的体内代谢、分泌调控和作用机制的研究进展予以综合评述。通过鱼类划性放射免疫测定法的建立和体风代谢动力学研究，证实，外源生长激素在鱼体内不会产生积累。鱼类生长激素的分泌调控受下丘脑分泌的生长激素释放因子（ＧＨＲＦ）和释放抑制因子（ＳＲＩＦ）的双重；促性腺激素释放激素（ＧｎＲＨ）、雌二醇（Ｅ２）、促甲状腺素释放激素（ＴＲＨ）和神经肽（ＮＹＰ）对鱼类生长激素的     

廉州湾及其邻近水域初级生产力研究

１９９２～１９９３年，在对廉州湾及其邻近海域的环境调查中，首次对该海区叶绿素ａ与初级生产力进行了测定。获得叶绿素ａ的春季平均值为１．００ｍｇ·ｍ－３，秋季为０．２９ｍｇ·ｍ－３，初级生产力的平均值为３０１．５ｍｇ·ｍ－２·ｄ－１。文章对调查区初级生产力的分布特征及变化规律进行了探讨，并与北方沿海的初级生产力水平进行了比较。     

大亚湾海域锥状斯氏藻赤潮及其与孢囊的关系

用沉积物捕捉器取样，研究了大亚湾海域２０００年６～９月孢囊（Ｃｙｓｔ）种群动态，同时 分析了浮游植物种群动态。研究期间（８～９月）该海域发生了一起大规模的锥状斯氏藻赤潮。 赤潮前一个月，锥状斯氏藻孢囊的平均日形成率为２．０５× １０５ｃｙｓｔｓ／（ｍ２·ｄ），丰度为５０．２２％， 该时期以有性生殖产生的静止期孢囊为主；在赤潮盛期的８，９月初，孢囊的平均日形成率达到 ２．０１ ×１０６ｃｙｓｔｓ／（ｍ２·ｄ），丰度为９６．９７％，此时期的孢囊多为营养细胞所形成的暂时性孢囊； 赤潮结束后，由于环境条件的恶化，暂时性孢囊的形成达到高峰，平均日形成率为１．２４×ｌ０７ ｃｙｓｔｓ／（ ｍ２·ｄ），丰度为９９.５３％。结果表明大亚湾丰富的孢囊是潜在的种源（Ｓｅｅｄ　ｂｅｄ），２０００年 的夏季、秋李持续高温（＞２５℃），台风减少及风力减弱等有利锥状斯氏藻的环境条件诱发孢 囊萌发，开始了该种类的赤潮。     

Numerical and simplified methods for the calculation of the total horizontal wave force on a perforated caisson with a top cover

The VOF method and the k–ε model, combined with the equation of state of air at constant temperature, have been used to calculate the total horizontal wave force caused by monochromatic waves acting on a perforated caisson with a top cover. From comparison of various parameters, such as the total horizontal force, the pressure difference on the front wall, the pressure on the back wall and the pressure on the top cover, between the numerical results and test data, it can be seen that the numerical results agree well with the test data. It is concluded that the method described in this paper can be utilized to calculate wave forces acting on perforated caissons with a top cover in the case of nonovertopping, nonbreaking waves. A simplified method to calculate the total horizontal force has been developed, based on test data, using a least-squares method. A comparison between the numerical results and the values calculated from the simplified equations shows good agreement. Therefore the simplified equations can be used in engineering applications to evaluate the total horizontal force on a perforated caisson with a top cover.     

Optimal Design for Main Dimensions of a 75000t Floating Production and Storage Vessel

The basis, process and results of the demonstration of the main dimensions of a 75000t floating production and storage vessel are discussed in this paper. A simple but reliable orthogonal design method is applied in the main dimension optimization. The ideas of gradual approximation and feedback from various aspects are put into effect. During the demonstration, in order to make the model tally with the actual situation, the draft design is closely related to the computational analysis, so that the demonstration model can be verified at any time; the handling of the overall system is closely related to the research of each item, which is beneficial not only to the mastery of various regularities, but also to the balance of decisions. Finally, according to the computational results and the regularities obtained from analysis, the main dimensions are determined.     

全球海洋与大气界面的熵通量估算

给出并讨论了海洋系统中由于热量交换、物质交换以及外力做功引起的熵通量的表达式;同时,根据新近调整的全球海 气界面的气候平均资料,估计了全球海洋与大气界面气候年平均的熵通量(熵流),这一熵通量主要取决于海 气界面的热量和物质交换的空间不均匀性。计算结果显示,由热量交换分量引起的熵通量对海洋系统的总熵通量起到主要的贡献作用,其值约为-555.6mW·(m2·K)-1;由海表风应力做功引起的熵通量相对较小,约为-0.09mW·(m2·K)-1;由物质交换引起的熵通量最小,仅为-0.02mW·(m2·K)-1。总的来说,海洋系统从外界获得的总熵通量为-555.7mW·(m2·K)-1,这也就意味着在气候平均定态下,海洋系统内部的熵产生在量值上等于系统的熵交换,即为555.7mW·(m2·K)-1。海洋系统的负熵流与其内部的各种不可逆过程引起的熵产生取得平衡,确保了全球海洋系统处于非平衡热力学定态,并维持着海洋系统中各种尺度的时 空有序现象的消长过程。     

Computation of Viscous Uniform and Shear Flow over A Circular Cylinder by A Finite Element Method

The incompressible viscous uniform and shear flow past a circular cylinder is studied. The two-dimensional Navier-Stokes equations are solved by a finite element method. The governing equations are discretized by a weighted residual method in space. The stable three-step scheme is applied to the momentum equations in the time integration. The numerical model is firstly applied to the computation of the lid-driven cavity flow for its validation. The computed results agree well with the measured data and other numerical results. Then, it is used to simulate the viscous uniform and shear flow over a circular cylinder for Reynolds numbers from lO0 to lO00. The transient time interval before the vortex shedding occurs is shortened considerably by introduction of artificial perturbation. The computed Strouhal number, drag and lift coefficients agree well with the experimental data. The computation shows that the finite element model can be successfully applied to the viscous flow problem.     

Variation in Water Masses on Shelf of Northern Bering Sea in September 2016-2018

Abstract

Based on hydrological data obtained during the 7th to 9th Chinese National Arctic Research Expeditions in the summers of 2016–2018, the main water structure on the shelf of the northern Bering Sea and the volume and heat fluxes of the Bering Strait throughflow were analyzed. Distinct variability was identified in the three Pacific water masses feeding the strait - Anadyr Water (AW), Bering Shelf Water (BSW) and Alaskan coastal water (ACW). Overall, the temperature and salinity of the entire section increased each year, with 2018 showing significant anomalies, i.e., a temperature anomaly of up to 1?°C and a maximum salinity anomaly of 2. From 2016 to 2018, the extent of the ACW gradually narrowed in the eastern part of this section, while the AW expanded eastward each year. The net volume transport through each of the three sections increased poleward from 1.65?Sv to 2.76?Sv, with the AW increasing from 0?Sv to 1.03?Sv, the BSW varying between 0.52–1.65?Sv, and the ACW gradually decreasing from 1.04?Sv to disappearing completely. The net heat fluxes were also poleward, varying between 25.77 TW and 61.50 TW, and showing a significant increase. Significant variations in magnitude and extent were observed in each water mass of the Bering Strait throughflow, which could produce widespread effects in the Arctic Ocean and the global ocean beyond.