Metabolism and mutagenicity of 4-nitroquinoline N-oxide by microsomes and cytosol of digestive gland of the mussel Mytilus edulis L.

The modulation of the mutagenicity of 4-nitroquinoline N-oxide (4-NQO) by M. edulis digestive gland cytosolic and microsomal fractions was studied by monitoring the induction of the umu operon by 4-NQO in Salmonella typhimurium 1535/pSK1002. The mutagenicity of 4-NQO was increased by M. edulis cytosol and microsomes in dose-dependent manner with respect to protein and toxicant concentration. The mutagenic enhancement reflected increased NAD(P)H-dependent oxygen consumption by microsomes in the presence of 4-NQO and enhanced nitroreductase activity in cytosol. The former was stimulated by azide, indicating 4-NQO-enhanced oxyradical production by microsomes, and the latter was inhibitable by dicoumarol, indicating involvement of DT-diaphorase in nitroreduction by M. edulis cytosol. Neither dicoumarol nor allopurinol (inhibitors of DT-diaphorase and xanthine oxidase, respectively) affected the enhanced mutagenic expression of 4-NQO by cytosol. The results support the possibility of oxidative stress as a pollution-mediated mechanism of toxicity.     

Temporal changes of mollusc populations from a Zostera marina bed in southern Spain (Alboran Sea), with biogeographic considerations

Molluscs associated with a Zostera marina bed from Cantarriján bay (Southern Spain, Alboran Sea) at 14–16 m depth were sampled monthly from October 1996 to September 1997. A total of 44,819 individuals belonging to 80 species were identified. In spite of the high species richness, only seven species of gastropods showed a dominance value (D) higher than 1%. Jujubinus striatus was the dominant species of the assemblage with 70.8% of the total abundance. The other dominant species were Rissoa membranacea (9.8%), Nassarius pygmaeus (5.8%), Mitrella minor (4%), Smaragdia viridis (1.9%), Rissoa monodonta (1.4%), Bittium reticulatum (1.3%). The dynamic pattern of the mollusc populations showed a temporal trend with monthly values of species richness and abundance ranging between 10 and 25 species and between 178 and 4412 individuals·222 m⁻². The species richness and abundance were higher in the spring and summer months than in the autumn and winter ones. The diversity (Shannon–Wiener, H’) follows a similar trend, with increases from April to September and decreases from October to March. H’ values (ranging from 0.45 to 3.10) are more influenced by the evenness (J) than by the species richness. A multivariate analysis (Cluster, Multi-dimensional Scaling) based on both presence/absence and quantitative data has also pointed out a temporal trend, with spring–summer samples significantly different from autumn–winter samples. The temporal changes in abundance seem related with the species’ biology, such as recruitment events, as well as to the canopy features and shoot density variation in the Zostera meadow. From the biogeographical point of view, most of the molluscs (65%) found in the Cantarriján bed, have a Lusitanian–Mediterranean distribution (sensu Ekman 1953 ). The proximity to Africa is shown by the presence of four species with a mainly West African distribution. Only R. membranacea has a typical Atlantic distribution, driven by that of Z. marina in NW Europe.     

Acute Disturbance of Lake Pontchartrain Benthic Communities by Hurricane Katrina

In 2005, Hurricane Katrina produced a distinct, acute ecological disturbance of the benthic invertebrate community of Lake Pontchartrain, LA, USA. The bivalve Rangia cuneata and other community dominants were lost from 50% (815 km²) of the lake bottom. The storm surge directly killed benthic organisms and produced salinity stratification that caused episodes of detrimental low dissolved oxygen concentration at depths >3.7 m. Past disturbance of the bottom by shell dredging and intrusion of higher salinity bottom water through deep shipping channels appears to have contributed to the severity of this impact. Colonization by tolerant opportunistic taxa occurred, but low rainfall after Katrina has slowed the recovery of the typical rangia community. A decrease in water transparency and an increase in turbidity and chlorophyll a were associated with the loss of clam biomass. Other hurricanes may have produced less obvious but similar effects on smaller spatial and temporal scales.     

Progress and Challenges in Coupled Hydrodynamic-Ecological Estuarine Modeling

Numerical modeling has emerged over the last several decades as a widely accepted tool for investigations in environmental sciences. In estuarine research, hydrodynamic and ecological models have moved along parallel tracks with regard to complexity, refinement, computational power, and incorporation of uncertainty. Coupled hydrodynamic-ecological models have been used to assess ecosystem processes and interactions, simulate future scenarios, and evaluate remedial actions in response to eutrophication, habitat loss, and freshwater diversion. The need to couple hydrodynamic and ecological models to address research and management questions is clear because dynamic feedbacks between biotic and physical processes are critical interactions within ecosystems. In this review, we present historical and modern perspectives on estuarine hydrodynamic and ecological modeling, consider model limitations, and address aspects of model linkage, skill assessment, and complexity. We discuss the balance between spatial and temporal resolution and present examples using different spatiotemporal scales. Finally, we recommend future lines of inquiry, approaches to balance complexity and uncertainty, and model transparency and utility. It is idealistic to think we can pursue a “theory of everything” for estuarine models, but recent advances suggest that models for both scientific investigations and management applications will continue to improve in terms of realism, precision, and accuracy.     

Hazard-consistent response spectra in the Region of Murcia (Southeast Spain): comparison to earthquake-resistant provisions

Hazard-consistent ground-motion characterisations of three representative sites located in the Region of Murcia (southeast Spain) are presented. This is the area where the last three damaging events in Spain occurred and there is a significant amount of data for comparing them with seismic hazard estimates and earthquake-resistant provisions. Results of a probabilistic seismic hazard analysis are used to derive uniform hazard spectra (UHS) for the 475-year return period, on rock and soil conditions. Hazard deaggregation shows that the largest hazard contributions are due to small, local events for short-period target motions and to moderate, more distant events for long-period target motions. For each target motion and site considered, the associated specific response spectra (SRS) are obtained. It is shown that the combination of two SRS, for short- and long-period ground motions respectively, provides a good approximation to the UHS at each site. The UHS are compared to design response spectra contained in current Spanish and European seismic codes for the 475-year return period. For the three sites analysed, only the Eurocode 8 (EC8) type 2 spectrum captures the basic shape of the UHS (and not the EC8 type 1, as could be expected a priori). An alternative response spectrum, anchored at short- and long-period accelerations, is tested, providing a close match to the UHS spectra at the three sites. Results underline the important contribution of the frequent, low-to-moderate earthquakes that characterize the seismicity of this area to seismic hazard (at the 475-year return period).     

The influence of vertical water balance on modelling Pantanal (Brazil) spatio‐temporal inundation dynamics

The current benchmark approach for mathematical modelling of floodplain hydrologic regime consists of dynamically coupling one‐dimensional (1D) and two‐dimensional (2D) models for flow routing along the main channel and the floodplain, respectively. For large‐scale sites, floodplain inundation may spread over hundreds of square kilometres and may last for many months and even influence seasonal floods in following years. This paper aims at evaluating the effect of vertical water balance representation on modelling a large‐scale floodplain. The Pantanal wetland (140 000 km²; Brazil) is simulated using a 1D/2D coupled model approach, which also considers the representation of vertical water processes over the floodplain. Four scenarios are simulated: Baseline (the reference scenario), NoVertBal (in which the vertical water balance over floodplain is turned off) and ETp+1 and ETp?1 scenarios, characterized by artificially increasing or decreasing daily potential evapotranspiration (ETp) by 1 mm, respectively. The results showed that the effect of the vertical water processes scenarios on channel flow is directly dependent on the lateral exchange of water between the channel and floodplain in the upstream river reach. This influence is stronger when there is a gain of water from the floodplain to the channel. The inclusion of these vertical water processes into floodplain modelling was essential to represent the process of wetting and drying, this effect being more relevant for areas not directly connected to main channels, which is a characteristic of the Pantanal region. Copyright © 2013 John Wiley & Sons, Ltd.     

OSL dating of the Middle Palaeolithic Hotel California site,Sierra de Atapuerca,north‐central Spain

In this study we present optically stimulated luminescence (OSL) dating results obtained at one of the most important open‐air Middle Palaeolithic sites in the Sierra de Atapuerca foothills – Hotel California. We also assess the possibility of obtaining extended‐range OSL chronologies for a nearby Middle Pleistocene fluvial deposit using several novel methods, namely OSL dating of individual quartz ‘supergrains’, multi‐grain aliquot thermally transferred OSL (TT‐OSL) dating and the first application of a single‐grain TT‐OSL dating procedure. Four single‐grain OSL ages constrain the Middle Palaeolithic occupation of Hotel California to between 71±6 and 48±3 ka. The Hotel California single‐grain equivalent dose (D_e) distributions are highly overdispersed and contain several dose populations, which are probably attributable to post‐depositional sediment mixing, partial bleaching and intrinsic scatter. The reliability of multi‐grain aliquot OSL dating is compromised by the complex underlying D_e dispersion affecting these samples, as well as by biasing multi‐grain averaging effects. Extended‐range OSL and TT‐OSL chronologies for the nearby Pico River terrace are consistent with each other and with broad independent age control. These experimental approaches yield a weighted average age of 348±16 ka for terrace T_A9 of the Arlanzón River sequence. Our results highlight the benefits of comparing ages obtained using several OSL methodologies to improve the robustness of luminescence chronologies. They also demonstrate the potential that single‐grain OSL techniques offer for establishing improved age constraints on the many other Middle Palaeolithic sites found at Atapuerca and elsewhere across north‐central Spain.     

The rise of the diptera‐microbial mat interactions during the Cenozoic: consequences for the sedimentary record of saline lakes

Shoreline gypsiferous sediments of an inland lake in central Spain furnishes valuable insight into reconstructions of early sedimentary changes related to shore fly–microbial mat interactions in fossil gypsum precipitating saline lake systems. The association of adult and larval forms of Ephydra (Diptera) with microbial matgrounds overlying the lake margin results in the formation of gypsiferous meniscate back‐filled burrows that provide an analogue for recurring, extensively developed trace fossils that occur in Cenozoic, but not older, lacustrine gypsum rocks. In this setting, sediment burrowing by ephydridae hinders significant preservation of microbialites. The overwhelming rise of Diptera at the onset of the Cenozoic resulted in extensive feeding and dwelling activity and contributed to reshape the saline aquatic habitat where microbial mats thrived, thus leading to the formation of specific trace fossils that are illustrative of the existence of microbes in the paleoenvironment.