Development of three multiplex PCR primer sets for ark shell (<Emphasis Type="Italic">Scapharca broughtonii</Emphasis>) and their validation in parentage assignment

Scapharca broughtonii is a commercially important and over-exploited species. In order to investigate its genetic diversity and population structure, 43 novel polymorphic microsatellites were isolated and characterized. The number of alleles per locus ranged from 3 to 22 with an average of 6.93, and the observed and expected heterozygosities varied between 0.233 and 1.000, and 0.250 and 0.953, with an average of 0.614 and 0.707, respectively. Three highly informative multiplex PCRs were developed from nine of those microsatellites for S. broughtonii. We evaluated and validated these multiplex PCRs in 8 full-sib families. The average polymorphism information content (PIC) was 0.539. The frequency of null alleles was estimated as 3.13% of all the alleles segregation based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 100% of all offspring were unambiguously allocated to a pair of parents based on 3 multiplex sets. Those 43 microsatellite loci with high variability will be helpful for the analysis of population genetics and conservation of wild stock of S. broughtonii. The 3 sets of multiplex PCRs could be an important tool of pedigree reconstruction, population genetic analysis and brood stock management.     

基于主成分分析的济宁市环境承载力研究

该文以济宁市环境承载力为研究对象,运用主成分分析法,从影响环境污染状况、环境治理状况、人口状况、经济发展状况、自然资源状况、物耗能耗状况等19个指标中,提取出影响环境承载力动态变化的3个主成分,对济宁市环境承载力年际变化进行了综合评价。结果表明:2006—2010年,济宁市环境承载力呈现逐年上升的趋势。该研究评价结果较好,可以为该地区的可持续开发规划提供理论参考。     

脐带缆在潜器下放过程中的运动建模与仿真

潜器在下放布置过程中需要考虑变长度的脐带缆对其运动的影响,在考虑流的情况下,对脐带缆的有限差分模型进行了改进,并采用最小二乘方法求解由时间和空间上的中心差分格式离散后的非线性方程组。为了验证模型改进的有效性,将潜器在均匀定常流中水平匀速直航时的数值解与该特殊情况下的解析解进行比较,两组解的吻合不仅证实了模型改进的有效性,而且表明上述数值计算方法是可靠且有效的。在潜器下放布置运动给定的情况下,脐带缆的运动仿真结果表明,潜器姿态角的调整会对拖曳点处的缆绳张力大小和变化趋势产生显著的影响,流在改变缆绳空间形状的同时引起了缆绳对潜器作用力和力矩的非线性和时变性,脐带缆总是阻止潜器自身动力对运动速度和姿态角的改变,且对纵荡运动的影响最大。     

广西近海灾害地质因素及声学反射特征

利用高分辨率地球物理资料及相关历史资料,对北部湾北部的广西近海灾害地质因素进行了地质地球物理解译,识别出海底沙波、海底沙脊、塌陷洼地、冲刷沟槽、浅层气、残积砂体、地层间断、埋藏丘、埋藏古河道等多种灾害地质因素.根据它们出现的频率、规模及声学特征可知:浅层气和埋藏古河道是广西近海最为发育的灾害地质因素,其对海洋工程的施工与建设有较大的危害性.     

A Preliminary Study on the Soft–Sediment Deformation Structures in the Late Quaternary Lacustrine Sediments at Tashkorgan, Northeastern Pamir, China

This study identified soft–sediment deformation structures(SSDS) of seismic origin from lacustrine sediments in the late Quaternary paleo–dammed lake at Tashkorgan, northeastern Pamir. The observed deformation structures include sand dykes, liquefied diapir and convolute structures, gravity induced SSDS, and thixotropic pillar and tabular structures. We conducted a preliminary study on the morphology, formation and trigger mechanisms of pillar and tabular structures formed by liquefaction of underlying coarse sand and thixotropy of the upper silty clay. The regional tectonic setting and distribution of lacustrine strata indicate that the most probable trigger for the SSDS in lacustrine sediments was seismic activity, with an approximate earthquake magnitude of M6.0; the potential seismogenic fault is the southern part of the Kongur normal fault extensional system. AMS 14 C dating results indicate that the SSDS were formed by seismic events occurring between 26050±100 yr BP and 22710±80 yr BP, implying intense fault activity in this region during the late Pleistocene. This study provides new evidence for understanding tectonic activity and regional geodynamics in western China.     

浙江新昌早白垩世木化石新材料及内含真菌菌丝化石

浙江新昌下白垩统馆头组是我国南方地区为数不多的白垩纪木化石产出层位。本文报道了新昌地区馆头组木化石新材料及其内含真菌菌丝化石。木化石具明显生长轮,管胞径壁纹孔南洋杉式(多数单列、椭圆形、紧挤排列,偶见双列互生排列);交叉场纹孔南洋杉式;木射线单列式,多数2~11个细胞高。当前木化石新材料具有与现生南洋杉科植物相类似的木材解剖特征,显示其应归入贝壳杉型木属(Agathoxylon Hartig)。鉴于该类型木化石的分类现状仍有待厘清,将其定为Agathoxylonsp.。木化石内含真菌菌丝化石,管状,具横隔,平直或弯曲,常以不同角度分叉,菌丝壁光滑,具典型的锁状联合,属于担子菌类。当前木化石木材组织具有明显的木材腐朽特征,与现代真菌造成的木材白腐一致,推断此真菌化石为木材白腐菌。新昌地区贝壳杉型木属木化石新材料的发现进一步丰富了馆头组木化石的多样性,有助于增进对南方地区早白垩世植物群组成特征的认识。当前真菌菌丝化石是我国南方地区首次发现白垩纪担子菌化石,为探究白垩纪时期森林生态系统真菌与植物的相互作用提供了新的化石证据。     

2000-2014年西藏雅鲁藏布江流域积雪时空变化分析及对气候的响应研究

利用2000-2014年MODIS逐日无云积雪产品对雅鲁藏布江流域积雪特征的空间分布及变化、积雪随高程变化的规律进行了分析,并采用被动微波数据SMMR （1979-1987年）和SSM/I （1988-2008年）以及中国地面降水和气温0.5°×0.5°日值格点数据集,研究了雅鲁藏布江流域关键积雪参数对气候要素的响应等。结果表明：流域下游积雪日较大且变化剧烈;流域整体上呈显著减少的趋势;积雪日随高程的上升而增加;流域内降水呈不显著的增加趋势,而气温呈显著的增加趋势,最高气温对积雪变化影响最大;气温对积雪终日的影响明显高于积雪初日;在积雪消融期降水的增多促进了积雪的消融。