Effects of hydrostatic pressure on microbial alteration of sinking fecal pellets

We used a new experimental device called PASS (PArticle Sinking Simulator) during MedFlux to simulate changes in in situ hydrostatic pressure that particles experience sinking from mesopelagic to bathypelagic depths. Particles, largely fecal pellets, were collected at 200 m using a settling velocity NetTrap (SV NetTrap) in Ligurian Sea in April 2006 and incubated in high-pressure bottles (HPBs) of the PASS system under both atmospheric and continuously increasing pressure conditions, simulating the pressure change experienced at a sinking rate of 200 m d⁻¹. Chemical changes over time were evaluated by measuring particulate organic carbon (POC), carbohydrates, transparent exopolymer particles (TEP), amino acids, lipids, and chloropigments, as well as dissolved organic carbon (DOC) and dissolved carbohydrates. Microbial changes were evaluated microscopically, using diamidinophenylindole (DAPI) stain for total cell counts and catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) for phylogenetic distinctions. Concentrations (normalized to POC) of particulate chloropigments, carbohydrates and TEP decreased under both sets of incubation conditions, although less under the increasing pressure regime than under atmospheric conditions. By contrast, dissolved carbohydrates (normalized to DOC) were higher after incubation and significantly higher under atmospheric conditions, suggesting they were produced at the expense of the particulate fraction. POC-normalized particulate wax/steryl esters increased only under pressure, suggesting biochemical responses of prokaryotes to the increasing pressure regime. The prokaryotic community initially consisted of 43% Bacteria, 12% Crenarchaea and 11% Euryarchaea. After incubation, Bacteria dominated (90%) the prokaryote community in all cases, with γ-Proteobacteria comprising the greatest fraction, followed by the Cytophaga–Flavobacter cluster and α-Proteobacteria group. Using the PASS system, we obtained chemical and microbial evidence that degradation by prokaryotes associated with fecal pellets sinking through mesopelagic waters is limited by the increasing pressure they experience.     

The missing layer: Geo-technologies, communities, and implications for marine spatial planning

The assessment and management of marine resources is an increasingly spatial affair dependent upon emerging geo-technologies, such as geographic information systems, and the subsequent production of diverse layers of spatial information. These rapid developments are, however, focused on biophysical processes and data collection initiatives; the social landscape of the marine environment is undocumented and remains a “missing layer” in decision-making. As a result, the resource areas upon which stakeholders and communities are dependent are neither mapped nor integrated into planning processes. We report on a participatory method to map the presence of fishing communities at-sea. The lessons learned concerning the spatial representation of communities informs not only fisheries, but other sectors struggling to incorporate similarly the human dimensions of the marine environment in assessment and planning.     

Interaction between nulls and emission in the pulsar B0834+06

We present a detailed study of the single pulses of the bright radio pulsar B0834+06, and offer evidence that the dominant periodic modulation in this pulsar's emission governs the occurrence of nulls. The nulls of B0834+06 constitute approximately 9 per cent of the total pulses and we demonstrate that they do not occur at random in the pulse sequence. On the contrary, they are found to occur preferentially close to the minimum of the pulsar's emission cycle, whose period jitters around a central value of P ₃≈ 2.17 rotation periods. It is likely that the intrinsic duration of the nulls averages about 0.2 times the pulsar rotation period. Surprisingly, the clearly distinct population of nulls and partial nulls of B0834+06 exhibit a two-peak profile slightly broader than that of the normal emission. This is in contrast to the profile of extremely weak normal pulses, which is narrower than the overall profile. A flow/counterflow model for the pulsar's two components can reproduce the essential observed features of the emission in its dominant mode, with nulls occurring at the point where the minima of the two systems are aligned. This suggests that the observed nulling rate is determined by the chance positioning of our sightline with respect to the system. If the flow is interpreted as part of a circulating carousel, a fit yields a best estimate of 14 'sparks'.     

Phase Transformations and Proton Promoted Dissolution of Hydrous Manganite (γ-MnOOH)

The objective of this study was to describe the proton promoted disproportion of synthetic manganite (γ-MnOOH) and to characterise the resulting phase transformations. The solution and remaining solid phase after disproportionation was analysed by techniques including atomic absorbance spectroscopy, X-ray diffraction (XRD), atomic force microscopy (AFM) and scanning electron microscopy (SEM). In suspensions with pH between 5 and 7, −log[H⁺] was monitored for 17 months and equilibrium constants were determined at 9, 12 and 17 months of reaction time for the following reaction (25 °C, 0.1 M (Na)NO₃):

The dispersal of pyroclasts from ancient explosive volcanoes on Mars: Implications for the friable layered deposits

A number of voluminous, fine-grained, friable deposits have been mapped on Mars. The modes of origin for these deposits are debated. The feasibility for an origin by volcanic airfall for the friable deposits is tested using a global circulation model to simulate the dispersal of pyroclasts from candidate source volcanoes near each deposit. It is concluded that the Medusae Fossae Formation and Electris deposits are easily formed through volcanic processes, and that the Hellas deposits and south polar pitted deposits could have some contribution from volcanic sources in specific atmospheric regimes. The Arabia and Argyre deposits are not well replicated by modeled pyroclast dispersal, suggesting that these deposits were most likely emplaced by other means.     

Geochemical comparison of woodlawn and mount painter acid volcanics,Southeastern Australia

Major‐ and trace‐element chemistry (including rare‐earth elements), total‐rock Rb‐Sr and U‐Pb and zircon U‐Pb data are used in an attempt to distinguish between two essentially coeval, felsic volcanic suites: the predominantly submarine Woodlawn suite which is associated with massive Cu‐Pb‐Zn sulphide mineralization and the terrestrial Mt Painter suite, with minor vein‐type mineralization. The Woodlawn samples are the unmineralized equivalents of the volcanics in the immediate ore environment.

Alteration perturbs some of the major‐ and trace‐element chemistry, particularly Ca and alkalis, thereby precluding their usefulness. REE patterns exhibit a significant light to heavy rare‐earth enrichment with an average La/Yb of 12 in the Mt Painter volcanics compared with 5.6 in the Woodlawn volcanics. Both suites have a marked negative Eu anomaly, with that of the Woodlawn samples more pronounced (‐45.5) than in the Mt Painter volcanics (‐29.2). A hydrothermally‐altered sample from Woodlawn has apparently lost about 50% of its light rare‐earth elements.

Initial ⁸⁷Sr/⁸⁶Sr ratios at about 0.711 are the same for rocks from both suites and differences in initial lead‐isotopic ratios appear negligible.

Zircons from both suites are a mixture of clear euhedral crystals and rounded discrete crystals or rounded cores overgrown by clear zircon. The U‐Pb data substantiate the morphological features in that the zircon suites both contain older inherited Pb but the Mt Painter zircons contain a greater proportion.

Cs concentrations and Cs/Rb and Ti/Zr ratios can be used to distinguish between the Woodlawn suite and the Mt Painter suite.     

Utilization of a submersible UV fluorometer for monitoring anthropogenic inputs in the Mediterranean coastal waters

We evaluated the performances of a submersible ultraviolet fluorometer (EnviroFlu-HC, TriOS Optical Sensors) dedicated to the real time measurement of polycyclic aromatic hydrocarbons (PAHs) in the aquatic media. We conducted calibration experiments and in situ measurements in the coastal Mediterranean Sea. We found that the EnviroFlu-HC was not strictly specific to PAHs, even though it exhibited the highest sensitivity for phenanthrene, but could response to tryptophan-like material as well, and in a much less extent, to humic substances. The sensor signal showed great spatial and temporal variations in clean and polluted sites, with likely a high contribution of PAHs in the harbors, and a high contribution of tryptophan-like and humic-like materials in the sewage effluent. We conclude that the EnviroFlu-HC is a good tool for monitoring anthropogenic inputs in the coastal waters, although its utilization should be combined to other fluorescence measurements to improve the information about the nature of the aromatic compounds detected.     

An intercomparison of observed and simulated extreme rainfall and temperature events during the last half of the twentieth century: part 2: historical trends

We analyze historical simulations of variability in temperature and rainfall extremes in the twentieth century, as derived from various global models run informing the Fourth Assessment Report of the Intergovernmental Panel on Climate Change (IPCC-AR4). On the basis of three indices of climate extremes, we compare observed and modeled trends in time and space, including the direction and significance of the changes at the scale of South America south of 10° S. The climate extremes described warm nights, heavy rainfall amounts and dry spells. The reliability of the GCM simulations is suggested by similarity between observations and simulations in the case of warm nights and extreme rainfall in some regions. For any specific extreme temperature index, minor differences appear in the spatial distribution of the changes across models in some regions, while substantial differences appear in regions in the interior of tropical and subtropical South America. The differences are in the relative magnitude of the trends. Consensus and significance are less strong when regional patterns are considered, with the exception of the La Plata Basin, where observed and simulated trends in warm nights and extreme rainfall are evident.