Transcriptome Analysis of Crassostrea sikamea (♀) × Crassostrea gigas (♂) Hybrids Under and After Thermal Stress

Crossbreeding is an effective approach to manage the genetic decline in aquaculture.One-way hybrids of Crassostrea sikamea(♀)and Crassostrea gigas(♂)have advantages in growth traits and adaptation to high temperature.Here,we used high-throughput sequencing to analyze the molecular processes in the hybrids under and after thermal stress.The hybrids were cultured in the seawater with an increasing temperature from 25℃to 40℃during 10 hours,which is regarded as the thermal stress stage.Then the temperature decreased from 40℃to 25℃within 2 h,which is regarded as the recovery stage.In this study,1293 significant diffe-rentially expressed genes(DEGs)were obtained under thermal stress,of which 576 were upregulated and 717 were downregulated,and 740 significant differentially expressed genes(DEGs)were obtained in the recovery stage,of which the number of upregulated and downregulated genes was 409 and 331,respectively.The antigen processing and presentation,NOD-like,and NF-kappa B path-ways were significantly enriched during the thermal stress stage.The MAPK and PPAR signaling pathways were significantly enrich-ed during the recovery stage.The HSP70,HSP90,and CANX genes were strongly and rapidly upregulated in the control/thermal stress groups but were slightly less upregulated in the thermal stress/recovery group.These results indicate that the innate immune system or nonspecific immunity was deployed to protect interior tissues from thermal stress.In addition,85%of the mutual DEGs were involved in bidirectional regulation(up/down or down/up)when the oysters were removed from the thermal stress to recover.This study provides preliminary insight into the molecular response of C.sikamea(♀)and C.gigas(♂)hybrids to thermal stress and provides a basis for future studies on temperature-adaptation and the possible expansion of hybrid breeding.     

Distribution and origin of brine-type Li-Rb mineralization in the Qaidam Basin,NW China

The Qaidam Basin on the Qinghai-Tibet Plateau is well endowed with a variety of critical metals,such as Li and Rb.However,little is known about the distribution,source,and enrichment mechanisms of these elements.In this paper,we present results of systematic geochemical and H-O-B isotopic analyses of brines in the Tertiary and Quaternary sediments of the Qaidam basin.The results reveal that the paleobrines from oil-bearing anticlines at Dafengshan,Nanyishan,Youquanzi,Jiandingshan,and Jianshishan are significantly enriched in Li and Rb,whose contents are 81.1-128.9 mg L^-1and 4.5-29.2 mg L^-1,respectively,reaching levels of economic significance.High Rb contents(up to 12.5 mg L^-1)and well-endowment of Li also characterize the Dongtai,Xitai,Yiliping,and Senie salt lakes in the Qaidam Basin.The Tertiary paleobrine average inδD andδ18O at-40.7‰and 2.84‰,respectively.The paleobrine with the highest Li and Rb hasδD andδ¹⁸O values close to magmatic water.Theδ¹¹B values are lower than those of river water but close to those of geothermal fluids,suggesting that replenishment by deep magmatic hydrothermal fluid is most likely the key mechanism for the anomalous enrichment of these elements in the paleobrines,though evaporation and water-rock reactions have contributed to the critical metal mineralization.The anomalous enrichment of Li and Rb in modern salt lake brines is closely related to the input of volcanic geothermal water.The relatively high Li and Rb contents of intercrystalline brines in salt lakes of the western Qaidam Basin and the increase of Li and Rb contents with increasing depth were caused by upwelling of local paleobrines along the fault zones.     

Relationship between the Extent of Igneous Rocks and Deep Structures as Determined by Gravitational and Magnetic Data in the South China Sea

The distribution of oil and gas resources in the South China Sea and adjacent areas is closely related to the structural pattern that helped to define the controlling effect of deep processes on oil-bearing basins.Igneous rocks can record important information from deep processes.Deep structures such as faults,basin uplift and depression,Cenozoic basement and magnetic basement are all the results of energy exchange within the earth.The study of the relationship between igneous rocks and deep structures is of great significance for the study of the South China Sea.By using the minimum curvature potential field separation technique and the correlation analysis technique of gravitational and magnetic anomalies,the fusion of gravitational and magnetic data reflecting igneous rocks can be obtained,through which the igneous rocks with high susceptibility/high density or high susceptibility/low density can be identified.In this study area,igneous rocks do not develop in the Yinggehai basin,Qiongdongnan basin,Zengmu basin and Brunei-Sabah basin whilst igneous rocks with high susceptibility/high density or high susceptibility/low density are widely-developed in other basins.In undeveloped igneous areas,faults are also undeveloped the Cenozoic thickness is greater,the magnetic basement depth is greater and the Cenozoic thickness is highly positively correlated with the magnetic basement depth.In igneously developed regions,the distribution pattern of the Qiongtai block is mainly controlled by primary faults,while the distribution of the Zhongxisha block,Xunta block and Yongshu-Taiping block is mainly controlled by secondary faults,the Cenozoic thickness having a low correlation with the depth of the magnetic basement.     

Integrated Radiolarian and Conodont Biostratigraphy of the Middle to Late Permian Linghao Formation in Northwestern Guangxi, South China

The Permian radiolarian zones and their correlations with conodont zones or other chronostratigraphic schemes are still under debate. In this study, four genera, 21 species and two subspecies of radiolarians together with one genus and six species of conodonts were recovered from the Linghao Formation cropping out at the Longwangpo (LWP) section, northwestern Guangxi, South China. Six radiolarian interval zones and one abundance zone are recognized in the section, namely in ascending order, the Follicucullus scholasticus, Albaillella cavitata, A. protolevis, A. levis, A. excelsa, A. triangularis Interval zones and A. yaoi Abundance Zone. They are correlated with the Clarkina dukouensis, C. guangyuanensis and C. orientalis conodont zones recognized at the same section. Based on our data, the F. scholasticus Interval Zone and the lowermost part of A. cavitata Interval Zone are recognized to be upper Capitanian age, whereas the four Albaillella Interval zones are of Lopingian age (Wuchiapingian to the late Changhsingian). Two previously known Changhsingian radiolarian zones, namely the A. triangularis and A. yaoi Interval zones, should be extended down to the uppermost Wuchiapingian in this studied section.     

Aquifer hydraulic conductivity prediction via coupling model of MCMC-ANN

Grain-size distribution data,as a substitute for measuring hydraulic conductivity(K),has often been used to get K value indirectly.With grain-size distribution data of 150 sets of samples being input data,this study combined the Artificial Neural Network technology(ANN)and Markov Chain Monte Carlo method(MCMC),which replaced the Monte Carlo method(MC)of Generalized Likelihood Uncertainty Estimation(GLUE),to establish the GLUE-ANN model for hydraulic conductivity prediction and uncertainty analysis.By means of applying the GLUE-ANN model to a typical piedmont region and central region of North China Plain,and being compared with actually measured values of hydraulic conductivity,the relative error ranges are between 1.55%and 23.53%and between 14.08%and 27.22%respectively,the accuracy of which can meet the requirements of groundwater resources assessment.The global best parameter gained through posterior distribution test indicates that the GLUEANN model,which has satisfying sampling efficiency and optimization capability,is able to reasonably reflect the uncertainty of hydrogeological parameters.Furthermore,the influence of stochastic observation error(SOE)in grain-size analysis upon prediction of hydraulic conductivity was discussed,and it is believed that the influence can not be neglected.     

Neoproterozoic–Early Paleozoic Tectonic Evolution of the South China Craton: New Insights from the Polyphase Deformation in the Southwestern Jiangnan Orogen

A >1500–km–long northeast–southwest trending Neoproterozoic metamorphic belt in the South China Craton (SCC) consists of subduction mélange and extensional basin deposits. This belt is present under an unconformity of Devonian–Carboniferous sediments. Tectonic evolution of the Neoproterozoic rocks is crucial to determining the geology of the SCC and further influences the reconstruction of the Rodinia supercontinent. A subduction mélange unit enclosed ca.1000–850–Ma mafic blocks, which defined a Neoproterozoic ocean that existed within the SCC, is exposed at the bottom of the Jiangnan Orogen (JO) and experienced at least two phases deformation. Combined with new (detrital) zircon U–Pb ages from metasandstones, as well as igneous rocks within the metamorphic belt, we restrict the strongly deformed subduction mélange as younger than the minimum detrital age ca. 835 Ma and older than the ca. 815 Ma intruded granite. Unconformably overlying the subduction mélange and the intruded granite, an intra–continental rift basin developed <800 Ma that involved abundant mantle inputs, such as mafic dikes. This stratum only experienced one main phase deformation. According to our white mica 40Ar/30Ar data and previously documented thermochronology, both the Neoproterozoic mélange and younger strata were exhumed by a 490–400–Ma crustal–scale positive flower structure. This orogenic event probably induced the thick–skinned structures and was accompanied by crustal thickening, metamorphism and magmatism and led to the closure of the pre–existing rift basin. Integrating previously published data and our new results, we agree that the SCC was located on the periphery of the Rodinia supercontinent from the Neoproterozic until the Ordovician. Furthermore, we prefer that the convergence and dispersal of the SCC were primarily controlled by oceanic subduction forces that occurred within or periphery of the SCC.     

Two New Species of Protocedroxylon Gothan (Pinaceae) from the Middle Jurassic of Eastern Inner Mongolia, NE China

Two new coniferous wood taxa, Protocedroxylon zhangii sp. nov. and P. zhalantunense sp. nov., are described from the Middle Jurassic Wanbao Formation in Zhalantun City, Inner Mongolia, NE China. The new discovery represents the first record of petrified wood in the Wanbao Formation, and contributes to further understanding the floral composition, especially that of the forest, of the Wanbao Formation. Anatomically, the two new species are characterized by having a mixed type of radial pitting and Abietineentüpfelung. In extant conifers, Abietineentüpfelung is considered to be characteristic of the wood of Pinaceae. The new discovery further indicates that the first occurrence of Abietineentüpfelung in conifer-like wood should be earlier than the Kimmeridgian. The palaeolatitudinal distribution pattern of Protocedroxylon indicates that the genus is a palaeobiogeographically consistent group, which was probably restricted to wetter and cooler temperate climates. In combination with distinct growth rings, the occurrence of Protocedroxylon suggests that a cool temperate and seasonal climate might have prevailed during the sedimentation period of the Wanbao Formation in eastern Inner Mongolia, NE China. Such a view is also in agreement with the palaeoclimatic conditions indicated by the plant megafossils of the Wanbao Formation.     

地理信息系统野外实习新模式与实习基地建设

结合近几年GIS实习教学效果,从野外实习模式、实习基地、实习内容等方面进行了全新的探索,提出从创新的角度出发,以培养学生创新思维和综合能力为契机,对地理信息系统的野外实习模式及实习基地建设进行了初探。     

Aeolian component records in lacustrine sediments and its atmospheric circulation significance for evolution of Qarhan salt lake,NE Qinghai-Tibetan Plateau

正1 Introduction The Qarhan Playa of Qaidam Basin is located at the northeastern QinghaiTibetan Plateau(QTP),in which thick lacustrine and evaporate sediments deposited since the late Pleistocene(Chen et al.,1985,1986).As surrounded by capacious Gobi and yardang fields and suffered strong westerly-northwesterly wind,abundant aeolian materials were trapped in these sediment sequences in the     

An Attempt to Date the Salt-mounds in the Lop Nor Playa

正1 Introduction The Lop Nor playa,located in the eastern end of the Tarim Basin,capped with a thick salt crust covering an area of approximately 5,500 km2(Ma 2007)that closely resembles a"Great Ear"in satellite images.Understanding the formation of the salt crust can provide important