Sediment bioavailable organic matter,deposition rates and mixing intensity in the Setúbal–Lisbon canyon and adjacent slope (Western Iberian Margin)

The role of the Setúbal–Lisbon canyon in accumulation and transport of labile organic matter from the coastal sea and ocean surface water towards the deep sea was assessed by investigating the distribution of organic matter of different quality in sedimentary aggregates and surface sediments of the canyon and adjacent slopes. Total hydrolysable amino acids (THAA) and organic carbon (Corg) were measured from aggregates, and contents of Corg, chlorophyll a (chl a), phaeopigments (phaeo), chloroplastic pigment equivalents (CPE) from sediments. As indices of organic matter (OM) quality THAA:Corg, degradation index (DI), chl a:phaeo, chl a:Corg and C:N ratio were determined. Sediment profiles of chl a and the isotope 210 of lead (²¹⁰Pb) were used as tracers in a transport model to estimate deposition rates and background levels of the tracers, and sediment mixing rates (Db). Whereas bulk Corg contents of canyon and slope sediments were practically similar at all depths, higher contents of THAA, chl a and CPE, as well as higher THAA:Corg, DI and chl a:Corg, in aggregates and sediments from the upper reaches of the canyon indicate that labile organic matter accumulates in the upper canyon. This is confirmed by higher chl a and ²¹⁰Pb deposition and Db calculated from the model. Hence, the Setúbal–Lisbon canyon, specially the upper region, acts as a natural trap of organic matter that is transported to the region via lateral transport and vertical settling from primary productivity. Organic matter might be further transported in downward canyon direction via rebound processes. The chl a and ²¹⁰Pb profiles reveal active sediment mixing by physical processes and/or animal reworking.     

Dissolved inorganic carbon (DIC) and its carbon isotopic composition in sediment pore waters from the Shenhu area,northern South China Sea

The Shenhu area is one of the most favorable places for the occurrence of gas hydrates in the northern continental slope of the South China Sea. Pore water samples were collected in two piston cores (SH-A and SH-B) from this area, and the concentrations of sulfate and dissolved inorganic carbon (DIC) and its carbon isotopic composition were measured. The data revealed large DIC variations and very negative δ ¹³C-DIC values. Two reaction zones, 0–3 mbsf and below 3 mbsf, are identified in the sediment system. At site SH-A, the upper zone (0–3 mbsf) shows relatively constant sulfate and DIC concentrations and δ ¹³C-DIC values, possibly due to bioturbation and fluid advection. The lower zone (below 3 mbsf) displays good linear gradients for sulfate and DIC concentrations, and δ ¹³C-DIC values. At site SH-B, both zones show linear gradients, but the decreasing gradients for δ ¹³C-DIC and SO₄ ²⁻ in the lower zone below 3 mbsf are greater than those from the upper zone, 0–3 mbsf. The calculated sulfate-methane interface (SMI) depths of the two cores are 10.0 m and 11.1 m, respectively. The depth profiles of both DIC and δ ¹³C-DIC showed similar characteristics as those in other gas hydrate locations in the world oceans, such as the Blake Ridge. Overall, our results indicate an anaerobic methane oxidation (AMO) process in the sediments with large methane flux from depth in the studied area, which might be linked to the formation of gas hydrates in this area.     

Protein carbonyls and antioxidant defenses in corkwing wrasse (Symphodus melops) from a heavy metal polluted and a PAH polluted site

The use of fish in environmental monitoring has become increasingly important in recent years as anthropogenic substances, many of which function as prooxidants, are accumulating in aquatic environments. We have measured a battery of antioxidant defenses as a measure of oxidative status, as well as protein carbonylation as a measure of oxidative damage, in corkwing wrasse (Symphodus melops) captured near a disused copper mine, where water and sediment are contaminated with heavy metals, and an aluminum smelter, a site contaminated with PAHs. Results were compared to two different reference sites. Fish at the heavy metal site had lower glucose-6-phosphate dehydrogenase activity and elevated protein carbonyls (1.8 times) compared to fish from the reference site. At the PAH site, EROD was increased 2-fold, while total glutathione and methemoglobin reductase concentration, were decreased. No differences were seen in protein carbonyl levels at the PAH site. Measures of both antioxidant defenses and oxidative damage should be used when assessing effects of xenobiotics on oxidative stress in fish species.     

Laboratory tests of vortex-induced vibrations of a long flexible riser pipe subjected to uniform flow

Laboratory tests have been conducted on vortex-induced vibration (VIV) of a long flexible riser towed horizontally in a wave basin. The riser model has an external diameter of 16 mm and a total length of 28.0 m giving an aspect ratio of about 1750. Reynolds numbers ranged from about 3000 to 10,000. Fiber optic grating strain gages are adopted to measure the dynamic response in both cross-flow and in-line directions. The cross-flow vibrations were observed to vibrate at modes up to 6 and the in-line reached up to 12. The fundamental response frequencies can be predicted by a Strouhal number of about 0.18. Multi-mode responses and the asymmetry of the bare pipe response in uniform flow were observed and analyzed. The experimental results confirmed that the riser pipe vibrated multi-modally despite it being subject to a uniform current profile and all of the excited modes vibrated at the Strouhal frequency. The asymmetrical distribution of displacement mainly resulted from the modal composition. Higher harmonics of the VIV response such as the third, fourth and fifth harmonics frequencies were found to be steady over the entire duration of the test even if they varied along the length of the riser pipe.     

Assessment of dissolved nutrients dispersal derived from offshore fish-farm using nitrogen stable isotope ratios (δ15N) in macroalgal bioassays

In this study, the dispersal of wastes from offshore fish farms was evaluated by analyzing nitrogen stable isotope ratios (δ¹⁵N) in macroalgae incubated in the water column at sites located at an increasing distance from the fish cages. Bioassays were performed at three fish farms situated in separate localities with different nutritional conditions (Canary Islands, Murcia and Catalonia) and varying in size, species of fish reared and annual production. Macroalgal bioassays were carried out in two different directions (DI and DII) and they were replicated at each distance in order to evaluate the effect of small-scale variability on the spatial extent of fish farm wastes. The results obtained with δ¹⁵N contribute to a better understanding of the application of nitrogen stable isotopes ratios in macroalgae as an effective bioindicator for tracing the dispersion of offshore fish farm wastes, and demonstrate that fish farm wastes can be traced even over distances of some km from the pollution source. In the Canary Islands, the maximum distance obtained for detection of fish farm wastes was between 450 and 700 m. Of the three installations studied, Murcia presented the greatest distance for detection of fish farm waste influence, ranging from between 1550 and 2450 m, whilst in Catalonia this distance was less than 120 m. In Catalonia, the results were masked by the influence of other sources of nitrogen, and thus fish farm wastes were detected at more reduced distances than expected. These results confirm that fish farm wastes can be traced using the nitrogen stable isotope ratios of macroalgae and that this method can also be useful for identifying areas of potential risk to some sensitive ecosystems, and as an early signal that changes in the community structure might occur.     

Automatic tracing of the foot of the continental slope

The UNCLOS III (Article 76, Section 4(b)) defines the foot of the continental slope as the point of maximum change in the gradient at its base. It is impossible to locate so defined afoot and thus to trace the foot‐line objectively by eye. In this study we show a method designed automatically to detect and trace the foot‐line of the continental slope from an irregular array of bathymetrical data. Our algorithm first transforms the bathymetric surface to a maximum curvature surface. On this new surface, the foot‐line corresponds to one of the ridges; instead of tracing the foot‐line on the bathymetric surface, we now can trace the ridges on the maximum curvature surface. The tracing of the ridges can be done automatically and objectively and the foot‐line is identified as being one of these ridges. We devote particular attention to the case when the ridge‐line is not defined, i.e., to the case when the point of maximum gradient change becomes a region of maximum gradient change.     

Comparison of vane shear and fall cone strengths of soft marine clay

A total of 1,014 measures of sediment shear strengths were measured by means of miniature vane shear and fall cone tests on five gravity cores collected in Eckernfo‐erde Bay, Baltic Sea. Paired t test was used to compare the shear strengths measured by the two methods. It was found that fall cone strength calculated with Wood's K₆₀value (0.29) overestimates the vane shear strength by 0.15 kPa (a = 0.001) and the sample mean of the fall cone strength is 4.1% higher than the mean of the vane shear strength. However, fall cone strength calculated with Hansbo's K₆₀ value (0.24) underestimates the vane shear strength by 0.88 kPa (a = 0.001), and the sample mean of the fall cone strength is 13.8% less than the mean of the vane shear strength. Both calculated fall cone strengths are significantly different from the vane shear strength, with a p value of less than 0.001. Regression analysis of the Echernfoerde Bay data indicates that a new K₆₀ value is 0.275 with a confidence interval (a = 0.01) from 0.2704 to 0.2786. Paired t test shows that there is no significant difference between miniature vane shear and fall cone tests for these samples if the fall cone strength is calculated with K₆₀ = 0.275.     

海洋沉积物孔隙水中阴阳离子含量的离子色谱法分析方法

分别采用Metrosep A Supp4-250型阴离子柱和Metrosep C 2-150型阳离子柱,以1.8 mmol/L Na2CO3 1.7 mmol/L NaHCO3和4 mmol/L酒石酸 0.75 mmol/L吡啶二羧酸为阴阳离子淋洗液,用离子色谱仪来测定海洋沉积物孔隙水中阴离子Cl-、Br-和SO42-浓度和阳离子Na 、NH4 、K 、Ca2 、Mg2 的浓度。特别是针对高Cl-和高Na 背景下Br-和NH4 的测定做了专门分析,得到了较理想的结果,建立了一套离子色谱法测定海洋沉积物孔隙水中常见的阴阳离子含量的实验方法。