管状蠕虫Ridgeia piscesae TAK1的克隆及其转基因果蝇制备

Ridgeia piscesae是唯一一种存在于东北太平洋Juan de Fuca Ridge热液区的管状蠕虫,我们成功通过RACE PCR获得Ridgeia piscesae TAK1(Rp TAK1)基因全长c DNA序列.为深入研究TAK1基因在管状蠕虫中的作用,本文通过显微注射获得Rp TAK1转基因果蝇,并在果蝇体内成功过表达Rp TAK1基因.我们发现由Rp TAK1编码的氨基酸序列具有一定保守性,可能暗示其功能上的保守性,同时也暗示TAK1蛋白在物种进化中的保守性.而过表达突变体果蝇的眼睛出现变异,包括眼睛变得粗糙、单眼排列不整齐及整体形状变小,可以看出Rp TAK1基因影响果蝇的机体发育,我们猜测在Rp TAK1基因在管状蠕虫的生长发育过程中也可能起到重要作用.     

华南埃迪卡拉(震旦)系顶部地层划分及与寒武系界线FAD分子的选择

"雪球事件"以来,早期生命呈现多层次、大规模的加速演化,到寒武纪前夕,生命大爆发达到高潮;在生命剧烈演替的同时,地球环境标志也发生着响应,碳同位素剧烈漂移,并且与各个生命演化节点相对应。但是在划分最重要的地质界线,埃迪卡拉(震旦)系——寒武系界线及界线层型和点位("金钉子",GSSP)确立的问题上迄今仍存在着较大的分歧,以遗迹化石Treptichnus pedum作为该GSSP的标志化石,因其跨系、跨阶产出,生物延限长,因而在全球的生物地层划分对比中(特别在西伯利亚和中国华南地区)一直质疑声不断,而且以遗迹化石作为标志化石本身也具有一定的缺陷。中国华南地区两类古老的宏体化石——矿化管状的Cloudina和条带状的Shaanxilithes在全球范围内可以很好的对比,并且这两种实体化石的出现都对应着碳同位素的明显正漂移。本文通过研究华南震旦系顶部化石带的地层分布和地理分布,综合分析了其时空分布特点和系统演化意义,深入探讨了Cloudina和Shaanxilithes作为寒武系底界标志化石所具备的潜力,对埃迪卡拉(震旦)纪—寒武纪地层界线划分和层型选择提出了更为理想的标准。     

Semi-analytical solutions for vertically and laterally loaded piles in multilayered soil deposits

In geotechnical practice, it is of considerable importance to assess the behavior of vertical–lateral coupled loading piles in multilayered soil deposits. This study deals with a semi-analytical formulation for the performance of a pile suffering from combined vertical and lateral loads. The emphasis is on quantifying the mobilization of the subgrade reaction provided by the layered soil stratums. In the proposed method, subgrade reactions, correlated with both the accumulative axial load transfer and side resistances depending upon the pile–soil interaction, are abstracted as a series of nonlinear springs in both vertical and lateral directions. On account of this, an alternative transfer matrix method is applied to characterize the pile reaction along the depth under the identified boundary conditions atop the pile; meanwhile, the condition of static equilibrium, between two specific pile segments located at the border of soil layers, is also essential. On this basis, validation of the solution is conducted by comparing with observations from experiments and predictions obtained from other existing methods. In addition, the influence of properties in shallow soil layer and the vertical load on the lateral response of the pile is also discussed. The results indicate a reduction in the lateral displacement and the maximum bending moment within the pile with the increase in the shallow soil stiffness, but a growth with the increase in vertical load due to the “P-Δ effect.”     

鄂尔多斯盆地中部马五7亚段颗粒滩沉积特征及空间分布

鄂尔多斯盆地中部马家沟组地层蕴含丰富的油气资源,以马五₇亚段为主要储集层位的盐下碳酸盐岩油气勘探近期取得了重要突破。基于钻井、测录井、岩芯及薄片等资料,对盆地中部马五₇亚段颗粒滩沉积特征、纵横向展布规律进行综合分析,进而探讨了研究区沉积古地理格局及其颗粒滩发育控制因素。结果表明:研究区马五₇亚段主要由晶粒白云岩及各类型颗粒白云岩构成,纵向上发育潟湖-颗粒滩-灰泥丘-台坪、潟湖-颗粒滩-滩间海-颗粒滩、潟湖-颗粒滩-灰泥丘三种沉积相序,颗粒滩位于向上变浅旋回的中上部,且常与微生物丘构成丘滩复合体建造,具有良好的储集意义。横向上,马五₇亚段发育两套较大规模的颗粒滩,层位稳定且连续性较好,整体表现为中西部厚而东部较薄的特征,平面上则呈南北向发育并沿东部凹陷环带状分布。研究区沉积规律表明马五₇亚段相对缺乏潮坪相沉积特征,推测其沉积环境更符合局限-蒸发台地。沉积期海平面的频繁变化控制了滩体发育规模及其垂向叠置样式,区内西高东底的古构造格局及其内部的微地貌起伏则决定了马五₇颗粒滩平面分布的差异性。     

Visual MODFLOW在煤矿建设地下水环境影响评价中的应用 ——以甘肃省灵台县安家庄煤矿为例

结合甘肃省灵台县安家庄煤矿地下水环境影响评价实例,建立了评价区水文地质概念模型,并基于Visual MODFLOW对评价区地下水进行了数值模拟计算和验证;预报了设计开采条件下研究区不同含水层的地下水水位。预报结果表明:矿坑排水使得煤层所在含水层出现疏干现象,并且疏干范围随着开采范围的增加而不断扩大;在煤矿开采作用下,随着模拟时间的延续,煤系层上覆白垩系洛河组—宜君组含水层地下水位降落漏斗的范围及地下水位下降幅度不断增大,但降落漏斗的范围基本上不超过采空区范围;煤矿开采对白垩系环河组含水层无影响,至矿区开采期结束,未引起含水层水位下降;对上部孔隙、裂隙潜水含水层也无影响,至矿区开采期结束未引起含水层水位下降。     

通过校企合作教育改进本科教学的实践与思考

校企合作教育是高校和企业联合,重点培养学生应用实践能力的一种有效机制和模式。本文以笔者研究团队近年来的合作教育实践为基础,剖析了合作教育中学校、学生、教师和企业的角色定位。通过分析总结合作单位和学生的实时反馈,发现了本科教学工作中存在的问题,提出有针对性的教学改进策略,以期提高合作教育的质量和效果,为培养专业应用创新型人才提供理论支持和实践参考。     

金衢盆地典型地区土壤-稻米重金属含量及土壤酸碱度的影响研究

作物对土壤中重金属的吸收受作物种类、采集部位及土壤理化性质等多方面因素的影响。近年来,金衢盆地土壤酸化面积逐年增大,酸化程度逐渐加深,其对土壤-作物系统中重金属元素的活动影响尚不明确。本文基于金衢盆地典型地区264组根系土壤-稻米样品分析数据,开展土壤、作物的重金属含量特征及其影响因素的研究,重点讨论了土壤pH对作物吸收重金属的影响。结果表明：①264件土壤中多数重金属元素的变异系数大于0.5,As、Cd、Cr、Cu、Ni和Zn元素之间呈显著正相关（P<0.01）。土壤Cd超标样品23件,超标率为8.7%;As、Cr、Cu、Hg、Ni、Pb和Zn超标样品均未超过2件。②稻米中Cu、Zn与Cd含量呈显著正相关,Cd的富集系数（BCF）高于植物营养元素Cu、Zn。③稻米中Zn和Cu在P<0.1水平上与pH值呈显著正相关。Cd、Cr、Hg的BCF与pH值之间存在一定的负相关性。研究认为,适当调低土壤的酸碱度会削减土壤中Cd、Hg等重金属元素的活性,从而减少农作物对重金属的吸收转运。研究结果可为当地粮食安全生产决策提供科学数据,为土地管护提供参考依据。     

Heat Flow Distribution and Thermal Mechanism Analysis of the Gonghe Basin based on Gravity and Magnetic Methods

Geothermal resource is indispensable as a clean, renewable, stable and cheap resource. Nowadays in China, the Gonghe Basin, located in northeastern Qinghai Province, has been thought to be a promising geothermal area. To explore geothermal energy potential in and around the Gonghe Basin, geophysical means including magnetic and gravity methods were used to plot distribution. Firstly, we inversed Moho depth and Curie point depth in and around the basin using gravity and magnetic data, respectively, through an improved Parker–Oldenburg algorithm. Secondly, seven different thermal models were established, considering radiogenic heat, basement depth, anomalous heat source and simulated corresponding temperature field and heat flow. These were analyzed numerically and we found the high heat flow in the Gonghe Basin co-acted with radiogenic heat, an anomalous heat source and conductive heat. The distribution of seismic activities indicates that the Langshan–Wuwei–Gonghe Fault might have provided channels for transporting heat effectively.