Effect of phenol on embryo development and expression of metallothionein in the sea urchin Hemicentrotus pulcherrimus

In this study, we identified and cloned the sea urchin Hemicentrotus pulcherrimus MT (Hp-MT) mRNA. We examined the gameto- and embryo-toxic effects and the expression of Hp-MT mRNA at various concentrations of phenol in H. pulcherrimus. We found that the normal embryogenesis rate was significantly inhibited when H. pulcherrimus was exposed to phenol (EC₅₀ = 1565.86 ppb, 95% Cl = 1183.47-2037.84 ppb). The no observed effective concentration (NOEC) and the lowest observed effective concentration (LOEC) of the normal embryogenesis rate were < 10 ppb and 100 ppb, respectively. Hp-MT cDNA is 651 bp in length and encodes a protein of 64 amino acids. We found that the expression of Hp-MT mRNA was significantly increased with phenol treatment in a concentrationdependent manner. These results suggest that phenol at greater than 100 ppb has a toxic effect during the early embryonic stages of H. pulcherrimus, and MT mRNA may be used as a biomarker for risk assessment of phenol contamination.     

Under-ice measurements of suspended particulate matters using ADCP and LISST-Holo

Ocean Science Journal - Using a mooring package comprising an acoustic Doppler current profiler (ADCP) and holographic imaging system, a 1-day ice camp study was performed under the Arctic sea ice...     

3-D Geovisualization of satellite images on smart devices by the integration of spatial DBMS,RESTful API and WebGL

Recent technological advancements in web-based geographic information systems have enabled access to satellite images on smart devices. The Representational State Transfer (REST) architecture overcomes difficulties that are associated with conventional data communications on the web, and the Web Graphics Library (WebGL) can be used as an alternative to web-based three-dimensional geographic visualization (3-D geovisualization) due to its efficient image processing capabilities. This paper describes a 3-D geovisualization system that was developed for satellite images on smart devices by integrating a spatial database management system (DBMS), a RESTful application programming interface (API), and WebGL. Spatiotemporal objects were constructed for time-series satellite images within a DBMS and a RESTful API was built for spatiotemporal queries to the time-series database so that the requested satellite data could be represented in 3-D on smart devices using WebGL. Satellite images that are represented in WebGL give a more realistic 3-D experience when they are combined with terrain data and provide for intuitive observations of the relationships between pixel values and associated geospatial conditions. This paper shows that a creative combination of existing technologies can be used to enhance and display satellite images on smart devices for 3-D geovisualization.     

The role of organic matter in the sorption capacity of marine sediments

Past studies have suggested that desiccation enhances hydrophobicity of salt marsh sediment, and that drying and rewetting sediment can be used to investigate sorption mechanisms of amino acids and other organic compounds [Liu, Z., Lee, C., 2006. Drying effects on sorption capacity of coastal sediment: The importance of architecture and polarity of organic matter. Geochim. Cosmochim. Acta 70, 3313–3324]. Here we further develop this technique to study sorption of hydrophobic and hydrophilic organic compounds in a wide range of marine sediments. Our results show that hydrophilic compounds sorb strongly to wet coastal sediments; in dried sediments, sorption of hydrophilic compounds decreases, while sorption of hydrophobic compounds is greatly enhanced. Small compounds with aromatic rings sorb more in dried than wet coastal sediments, suggesting that aromatic groups have a stronger effect on sorption than polar groups like amino and carboxyl moieties. Sorption of lysine, glutamic acid and putrescine decreases greatly when sediment is pretreated with KCl, indicating the importance of cation ion exchange. However, α-amino acids sorb much more than corresponding β- or γ-amino acids, and l-alanine sorbs more than d-alanine, suggesting that amino group location and chiral selectivity play an important role in sorption. Comparison of lysine and tyrosine sorption in different sediments indicates that source and diagenetic state of organic matter are important factors determining sorption capacity. Lysine sorbs much more to organic detritus from salt marsh sediment than to fresh Spartina root materials, marine particles, lignin or humic acids, indicating the importance of structural integrity in sorption. Desorption hysteresis of glutamic acid, putrescine and lysine (in dried sediment) suggests the presence of enzyme-type sorption sites of high sorption energy or multiple binding mechanisms. Taken together, these findings suggest that organic matter plays the major role in amino acid sorption in organic-rich sediments.     

Influence of salinity and fish species on PAH uptake from dispersed crude oil

The use of chemical oil dispersants to minimize spill impacts causes a transient increase in hydrocarbon concentrations in water, which increases the risk to aquatic species if toxic components become more bioavailable. The risk of effects depends on the extent to which dispersants enhance the exposure to toxic components, such as polycyclic aromatic hydrocarbons (PAH). Increased salinities can reduce the solubility of PAH and the efficiency of oil dispersants. This study measured changes in the induction of CYP1A enzymes of fish to demonstrate the effect of salinity on PAH availability. Freshwater rainbow trout and euryhaline mummichog were exposed to water accommodated fractions (WAF), and chemically-enhanced water accommodated fractions (CEWAF) at 0 per thousand, 15 per thousand, and 30 per thousand salinity. For both species, PAH exposure decreased as salinity increased whereas dispersant effectiveness decreased only at the highest salinity. Hence, risks to fish of PAH from dispersed oil will be greatest in coastal waters where salinities are low.     

Disorder and the extent of polymerization in calcium silicate and aluminosilicate glasses: O-17 NMR results and quantum chemical molecular orbital calculations

Estimation of the framework connectivity and the atomic structure of depolymerized silicate melts and glasses (NBO/T > 0) remains a difficult question in high-temperature geochemistry relevant to magmatic processes and glass science. Here, we explore the extent of disorder and the nature of polymerization in binary Ca-silicate and ternary Ca-aluminosilicate glasses with varying NBO/T (from 0 to 2.67) using O-17 NMR at two different magnetic fields of 9.4 and 14.1 T in conjunction with quantum chemical calculations. Non-random distributions among framework cations (Si and Al) are demonstrated in the variation of relative populations of oxygen sites with NBO/T. The proportion of non-bridging oxygen (NBO, Ca-O-Si) in the binary and ternary aluminosilicate glasses increases with NBO/T. While the trend is consistent with predictions from composition, the detailed fractions apparently deviate from the predicted values, suggesting further complications in the nature of polymerization. The proportion of each bridging oxygen in the glasses also varies with NBO/T. The fractions of Al-O-Si and Al-O-Al increase with increasing polymerization as CaO is replaced with Al₂O₃, while that of Si-O-Si seems to decrease, implying that activity of silica may decrease from calcium silicate to polymerized aluminosilicates (X_SiO2=constant). Quantum chemical molecular orbital calculations based on density functional theory show that a silicate chain with Al-NBO (Ca-O-Al) has an energy penalty (calculated cluster energy difference) of about 108 kJ/mol compared with the cluster with Ca-O-Si, consistent with preferential depolymerization of Si-networks, reported in an earlier O-17 NMR study [Allwardt, J., Lee, S.K., Stebbins, J.F., 2003. Bonding preferences of non-bridging oxygens in calcium aluminosilicate glass: Evidence from O-17 MAS and 3QMAS NMR on calcium aluminate glass. Am. Mineral.88, 949-954]. These prominent types of non-randomness in the distributions suggest significant chemical order in silicate glasses that leads to a decrease in silica activity coefficient and will be useful in modeling transport properties of melts.     

Imag(in)ing the continental lithosphere

This paper is primarily concerned with seismically imaging details in the mantle at an intermediate scale length between the large scales of regional and global tomography and the small scales of reflection profiles and outcrops. This range is roughly 0.1–1 km < a < 10–10² km, where a is the scale. We consider the implications of several models for mantle evolution in a convecting mantle, and possible scales present in the non-convecting tectosphere. Reflection seismic evidence shows that the structures preserved from continental accretion within and at the margins of the Archean cratons are subduction related, and we use subduction as an analog for scales left by past events. In modern orogenic belts we expect to find subduction structures, small scale upper mantle convection structures, and basalt extraction structures. We examine some of the scales that are likely formed by orogenic processes.We also examine the seismic velocity and density contrasts expected between various upper mantle constituents, including fertile upper mantle, depleted upper mantle, normal and eclogitized oceanic crust, and fertile mantle with and without partial melt. This leads directly to predicting the size of seismic signals that can be produced by specular conversion, and scattering from layers and objects with these contrasts.We introduce an imaging scheme that makes use of scattered waves in teleseismic receiver functions to make a depth migrated image of a pseudo-scattering coefficient. Image resolution is theoretically at least an order of magnitude better than traveltime tomography. We apply the imaging scheme to three data sets from 1) the Kaapvaal craton, 2) the Cheyenne Belt, a Paleoproterozoic suture between a protocontinent and an island arc, and 3) the Jemez Lineament, a series of aligned modern volcanic structures at the site of a Proterozoic suture zone. The Kaapvaal image, although not defining a unique base of the tectosphere, shows complicated “layered” events in the region defined as the base of the tectosphere in tomography images. The image of the transition zone discontinuities beneath the Kaapvaal craton is remarkable for clarity. The migrated receiver function image of the upper mantle beneath the Cheyenne belt is complicated, more so than the tomography image, and may indicate limitations in the receiver function imaging system. In contrast the Jemez Lineament image shows large-amplitude negative-polarity layered events beneath the Moho to depths of 120 km, that we interpret as melt-containing sills in the upper mantle. These sills presumably feed the Quaternary–Neogene regional basaltic volcanic field.     

Radon distribution in groundwater of Taiwan

Radon levels were surveyed in 517 monitoring wells constructed in five major groundwater areas of Taiwan. The radon concentration in groundwater samples varied in a wide range from below the detection limit of 18 pCi/L up to 1,100 pCi/L. A worldwide comparison of reported groundwater radon levels was conducted. Overall radon levels in Taiwan groundwater are relatively low compared to other countries because the geology of Taiwan is mainly comprised of sedimentary rocks. Among the 517 wells monitored, only five wells were found with radon concentrations higher than 500 pCi/L. These five wells are all located near the Chaochou Fault in the Pingtung Plain. This study suggests that well sites near the Chaochou Fault could be good locations to monitor radon anomalies for earthquake prediction and should be avoided for developing domestic water supply. In the recharge area near the Chaochou Fault, the radon concentration in groundwater from shallow wells was approximately 1/2 to 1/4 of that from deep wells in the same cluster.

---

Resumen Se investigaron los niveles de radón en 517 pozos de monitoreo, construidos en las cinco mayores áreas de agua subterránea de Taiwan. La concentración de radón en las muestras de agua subterránea varía en un rango amplio, desde inferior al límite de detección que es 18 pCi/L hasta 1,100 pCi/L. Se realizó una comparación a escala mundial de los niveles de radón reportados en agua subterránea. En general los niveles de radón presente en el agua subterránea de Taiwan, son relativamente bajos comparados con otros países, puesto que la geología de Taiwan está compuesta de rocas sedimentarias. Entre los 517 pozos monitoreados, solamente en cinco se encontraron concentraciones de radón mayores a 500 pCi/L. Estos cinco pozos están todos localizados cerca de la falla ChaoChou en la Planicie de PingTung. El presente estudio sugiere que los pozos cercanos a la falla Chaochou, podrían ser buenos sitios para monitorear anomalías de radón para la predicción de terremotos y deberían evitarse para los desarrollos de abastecimiento de agua potable. En la zona de recarga cerca de la falla Chaochou, la concentración de radón en agua subterránea obtenida en pozos someros, fue aproximadamente de &frac; a &frac; de aquella en pozos profundos ubicados en el mismo sector.

---

Résumé On a mesuré les taux de Rn dans 517 forages ouverts dans cinq aquifères majeures de Taiwan. Les concentrations en Rn couvrent un domaine très large, à partir de valeurs très basses, au dessous de la limite de détection de 18pCi/l jusquaux valeurs assez grandes de 1100pCi/l. On a mené une analyse comparative avec des valeurs mentionnées en littérature. Par report aux valeurs mesurées en autres pays, les concentrations en Rn en Taiwan sont asses basses, compte que la structure géologique de Taiwan est constituée surtout par des roches sedimentaires. On a trouvé des concentrations au desuss de 500pCi/l seulement en cinq de 517 forages investigués. Tous ces cinq forages se trouvent au voisinage de la faille de Chaochou dans la plaine de Pingtung. Cet étude suggere que les forages situés près de la faille de Chaochou peuvent bien monitoriser les anomalie de Rn pour la prédiction des séismes mais doivent être évités lorsqil sagit de lalimentation en eau potable. Dans la zone de recharge près de la faille de Chaochou les concentrations en Rn mesurées dans les puits peu profondes representent 1/2-1/4 de ceux mesurées dans les forages de profondeur.