凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析

采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。     

Cu2+、Zn2+、SDS、DBS对脊尾白虾的毒性试验

选用铜、锌2种常见重金属和十二烷基硫酸钠(SDS)、十二烷基苯磺酸钠(DBS)2种阴离子表面活性剂对脊尾白虾Palaemon carincauda仔虾进行96h急性毒性试验。结果表明,Cu2 、Zn2 、SDS、DBS对脊尾白虾仔虾的96h LC50分别为0.34、0.82、14.3和14.4 mg.L-1。将脊尾白虾仔虾对这4种毒物的敏感性与文献报道的其它动物进行了比较,结果认为可以将其作为毒性测试的标准试验生物。     

Complexation of mercury by dissolved organic matter in surface waters of Galveston Bay, Texas

The chemical speciation of dissolved mercury in surface waters of Galveston Bay was determined using the concentrations of mercury-complexing ligands and conditional stability constants of mercury-ligand complexes. Two classes of natural ligands associated with dissolved organic matter were determined by a competitive ligand exchange-solvent solvent extraction (CLE-SSE) method: a strong class (L_s), ranging from 19 to 93 pM with an average conditional stability constant (K_HgLs) of 10²⁸, and a weak class (L_w) ranging from 1.4 to 9.8 nM with an average K_HgLs of 10²³. The range of conditional stability constants between mercury and natural ligands suggested that sulfides and thiolates are important binding sites for dissolved mercury in estuarine waters. A positive correlation between the estuarine distribution of dissolved glutathione and that of mercury-complexing ligands supported this suggestion. Thermodynamic equilibrium modeling using stability constants for HgL, HgCl_x, Hg(OH)_x, and HgCl(OH) and concentrations of each ligand demonstrated that almost all of the dissolved mercury (> 99%) in Galveston Bay was complexed by natural ligands associated with dissolved organic matter. The importance of low concentrations of high-affinity ligands that may originate in the biological system (i.e., glutathione and phytochelatin) suggests that the greater portion of bulk dissolved organic matter may not be important for mercury complexation in estuarine surface waters.     

利用X射线衍射测定单斜辉石中硬玉组分含量

硬玉是单斜辉石中十分重要的端元组分,是单斜辉石分类的主要基础。利用x射线衍射分析确定的硬玉组分含量计算式为Jd(％)=[A-2.235]×25.741,其中Jd为硬玉组分百分含量,A为2—Theta角为30°(d=2.97)时峰值的相对强度Ⅰ与半高宽的乘积,其计算误差仅6.32％。     

青海钩虾配合饲料对罗非鱼两品系生长效果的研究

研究青海钩虾对罗非鱼两品系吉富罗非鱼和彩虹鲷生长的促进作用。实验用青海钩虾与面粉、麸皮和豆饼制成 5组 ( ～ )配合饲料及良种场原用饲料 ( 组 )饲育吉富罗非鱼和彩虹鲷 ,经2 5 d试验结果表明 :( 1)彩虹鲷增重效果以 组饲料最高 ,平均增重为 4 4 .9g,较大于 组的 4 3.1g;( 2 )所有钩虾饲料对彩虹鲷的增色效果都高于良种场的原用饲料 组 ,其中以饲料 组最高 ;( 3)饲料 I组饲育吉富罗非鱼平均增重为 4 4 g,高于 组的 4 0 .1g;( 4 )吉富罗非鱼和彩虹鲷比较则显示出吉富罗非鱼的增重率明显高于彩虹鲷而饵料系数又较低的结果。     

威海地区高压岩石组合及其成因演化

利用矿物地质温压计估算变质高峰期温压条件、退变质作用温压条件。研究表明,本区为鲁苏构造带的北延部分,代表本区晚元古代华南陆块与华北陆块的碰撞及三叠纪华北陆块向华南陆块推覆事件的发生和演化。     

雌雄比对海带雌配子体发育的影响

本实验进行了不同雌雄比对海带雌配子体发育的影响。结果表明：雌雄比(鲜重)为1：1及2：1，实验组出现排卵时间最早，且发育率高，实验第20天，发育率超过70％，孢子体正常。5：1，10：1，20：1实验组随着雄性比例的减少，发育率逐渐降低，随着培养天数的增加，三个实验组均出现未受精卵及畸形孢子体，雌雄比越大，未受精卵数目越多。     

Molecular analysis of complete ssu to lsu rdna sequence in the harmful dinoflagellatealexandrium tamarense (korean isolate, HY970328M)

New PCR primers (N=18) were designed for the isolation of complete SSU to LSU rDNA sequences from the dinoflagellateAlexandrium tamarense. Standard PCR, employing each primer set selected for amplifications of less than 1.5 kb, successfully amplified the expected rDNA regions of A. tamarense (Korean isolate, HY970328M). Complete SSU, LSU rDNAs and ITS sequences, including 5.8S rDNA, were recorded at 1,800 bp, 520 bp and 3,393 bp, respectively. The LSU rDNA sequence was the first report inAlexandrium genus. No intron was found in the LSU rRNA coding region. Twelve D-domains within the LSU rDNA were put together into 1,879 bp (44.4% G+C), and cores into 1514 bp (42.8% G+C). The core sequence was significantly different (0.0867 of genetic distance, 91% sequence similarity) in comparison withProrocentrum micans (GenBank access. no. X16108). The D2 region was the longest in length (300 bp) and highly variable among the 12 D-domains. In a phylogenetic analysis using complete LSU rDNA sequences of a variety of phytoplankton,A tamarense was clearly separated with high resolution against other species. The result suggests that the sequence may resolve the taxonomic ambiguities ofAlexandrium genus, particularly of the tamarensis complex.     

南海晚第四纪浮游有孔虫类群变化所表征的古海洋学特征

通过对南海4个岩心的74个样品进行Q型因子分析,求得4个浮游有孔虫组合:亚热带-热带组合,温凉组合,热带易溶组合和亚热带组合。亚热带-热带组合为优势组合,它的发育状况反映了晚第四纪西太平洋热带海水对南海的影响程度,其因子载荷作为古海流特征的参数。温凉组合的发育与南海存在冷涡有关。利用转换函数FP-12E计算了南海南部表层古水温,冰期与间冰期的表层水温平均变化冬季为4.7℃和2.9℃,夏季为3.0℃和2.1℃,季节性温差冰期最大可达8.6℃,间冰期最大可达5.0℃,均比太平洋同一纬度海区的大。冬季表层古水温波动比夏季的大。全新世的表层水温呈上升趋势,但在NS86-43柱中有一变冷现象,这可能和the younger Younger Dryas有关。     

海洋细菌抑菌活性菌株的筛选

从不同海区的海洋底质，生物样，水样中分离到480株海洋细菌，其中包括150株芽孢细菌和108株粪大肠杆菌，利用纸片法对其抑菌活性进行检测，结果表明：117株海洋细菌具有抑菌活性，占总分离菌株的24.4%；一般细菌，芽孢细菌，粪大肠杆菌的抑菌活性菌株分别占各供测菌株的17.1%,27.3%,35.2%；抑菌活性菌株的比例与样品来源密切相关，且各类海洋细菌的抗菌谱有很大的不同。