Profiling Planctomycetales diversity with reference to anammox‐related bacteria in a South China Sea,deep‐sea sediment

A systematic assessment of Planctomycetales diversity in a South China Sea, deep‐sea sediment (1657 m) was conducted using the 16S rRNA gene analysis approach. PCR amplification of the samples from seven sediment layers (0.1, 1, 3, 5, 7, 9 and 11 m below the surface sediment) using the primer set Pla‐46‐F/1392‐R showed that the Planctomycetales existed within a limited range of sediment depths (≤ 5 m), and had a decreasing trend in diversity with increasing depth. The majority of the retrieved Pla‐46‐F/1392‐R sequences belonged to Pirellula‐related Planctomycetales, and two sequences retrieved from the 0.1‐m layer (GenBank accession numbers: DQ996944 and DQ996945 ) shared the same anammox‐related signature oligonucleotides and were closely related to commonly recognized anammox organisms. To identify new anammox‐related biomarkers, three primer sets were designed for amplifying the fragments of hydroxylamine oxidoreductase and S‐adenosylmethionine radical enzyme genes, but no related sequences were found. Our multiple 16S rRNA gene primer sets (Journal of Rapid Methods and Automation in Microbiology, 2008, in press) revealed even an higher diversity of Planctomycetales in the 0.1‐m layer of the sediment, especially at genus level. Our data profiled the distribution pattern of Planctomycetales diversity along sediment depths, and provided molecular evidence for the existence of anammox‐related bacteria in a new location, which broadens our understanding of Planctomycetales diversity in deep sea sediments.     

Evaluation of ENVISAT ASAR data for sea surface wind retrieval in Hong Kong coastal waters of China

The C-band wind speed retrieval models, CMOD4, CMOD - IFR2, and CMOD5 were applied to retrieval of sea surface wind speeds from ENVISAT （European environmental satellite） ASAR （advanced synthetic aperture radar） data in the coastal waters near Hong Kong during a period from October 2005 to July 2007. The retrieved wind speeds are evaluated by comparing with buoy measurements and the QuikSCAT （quick scatterometer） wind products. The results show that the CMOD4 model gives the best performance at wind speeds lower than 15 m/s. The correlation coefficients with buoy and QuikSCAT winds are 0.781 and 0.896, respectively. The root mean square errors are the same 1.74 m/s. Namely, the CMOD4 model is the best one for sea surface wind speed retrieval from ASAR data in the coastal waters near Hong Kong.     

东营凹陷南斜坡沙四段高频沉积旋回对滩坝砂体的沉积控制作用

在东营凹陷南斜坡的沙四段地层中,常发育典型的湖相滩坝砂体沉积.详细观察表明,这些滩坝砂体在垂向上常发育较为对称的高频基准面上升半旋回和下降半旋回,且对滩坝砂体的沉积过程有明显的控制作用.通过测井曲线的频谱分析,高频基准面的变化与米兰柯维奇轨道气候周期有着密切的关系,而单砂体规模的滩坝砂体在平面展布上又有着明显的对称性.这些都表明滩坝砂体主要沉积在湖平面升降转换的相对稳定期,同生断层活动产生的陡坎或隆起会有助于滩坝砂体的富集.可根据滩坝砂体的垂向沉积序列和组合方式来判断滩坝主体的分布位置.     

栖霞市马家窑金矿床成矿流体地球化学特征

马家窑金矿床是胶东地区比较典型的石英脉型金矿床代表,属中低温热液矿床,成矿流体盐度较低,气相成分以H2O,CO2为主,其次是Na^＋,Ca^＋和Cl^-,K^＋和F^-较贫,流体的成分与大气降水热液相类似。成矿流体的H,O同位素值则反映大气降水在不同温度和不同水／岩值条件下与栖霞超单元回龙夼单元交换后的分布特征,认为马家窑金矿床的成矿热液属大气降水成因。     

3-甲基胆蒽对斑马鱼组织病理效应的初步研究

分别用25、50、100、200和400μg/L的3-甲基胆蒽(3-MC)对斑马鱼进行水浴染毒,于第7天和第14天采集各染毒组和对照组活鱼的肝、鳃、心、肠和肾,进行病理组织学检查。结果发现,一定剂量的3-MC能导致肝细胞变性,胞浆内出现脂肪滴,严重时肝细胞坏死解体,界限模糊;心肌细胞萎缩坏死;肠粘膜上皮细胞纹状缘不整齐,有的脱落;鳃丝结构异常,鳃小片表面粗糙,上皮细胞不完整;肾小管上皮细胞肿胀,细胞界限不清,管腔内均质红染。供试斑马鱼的病理组织变化与3-MC的作用剂量和作用时间存在一定的关系。     

基于nRF9E5的无线网络控制应用设计方案

结合现代餐饮业自动化服务的需求,以无线Soc芯片nRF9E5和点阵型LCD模块ST7920为硬件核心,结合C++Builder软件编程环境,构建了一种基于nRF9E5芯片的无线网络自动化服务管理系统,该系统具有人机界面友好、数据管理软件完善、无线数据传输与控制稳定、高效等特点。可以推广应用到区域水质、土壤等环境的自动监测等领域。     

Application on drilling parameter monitor in drilling engineering monitoring

The drilling parameter monitor is an important tool in drilling engineering applied to monitoring drilling process, carrying out scientific analysis and decision-making. Based on discussing the present development situation of the domestic and foreign drilling machine parameter monitors, the metering scheme for vehicle-loaded drilling parameter monitor was designed. By using detection system for MSP430 single-chip microcomputer (SCM) in combination with peripheral circuit such as sensors, the drilling-rig control system was obtained to detect, and for every parameter in real-time display in order to keep operating the drilling rig status. The experiment shows that the drilling parameter monitor reaches design requirements and can be applied to drilling engineering monitoring, which has characters such as simple structure, high credibility and low cost.     

几种环境因子对虾蟹类非特异性免疫的影响

综述了近10a国内外有关重金属、氨态氮、亚硝酸盐、有机污染物等几种环境因子对虾蟹类非特异性免疫影响的研究成果,以期为通过改善养殖环境条件提高虾蟹类自身免疫抗病力提供理论依据.同时,为进一步深入开展该领域的研究工作积累资料.     

水产饲料中镉的存在形态及其在养殖动物体内累积状况的研究

镉是对动物和人类健康有害的重金属元素,其毒性与存在形态密切相关.本文对水产饲料和原料中的含镉量、及其在不同提取剂的提取量进行测定,探讨其主要存在形态;测定结果表明水产配合饲料及其原料中的镉主要以蛋白结合态存在.以乌贼内脏粉为镉源调制的不同含镉量饲料对鱼、虾、蟹的饲喂对比试验,结果进一步证实,以Cd-MT 为主体的有机结合态镉对水产动物的毒理效应和代谢作用与离子态镉(Cd2 )存在着明显的差异,其在水产动物肌肉的再累积不明显,未发现对养殖动物的毒害作用和Cd的累积放大现象.     

宽壳全海笋的染色体核型分析

以宽壳全海笋担轮幼虫为材料,采用热滴片法进行染色体核型分析.结果表明:宽壳全海笋二倍体染色体数目为2n=38,染色体臂数(NF)=70,核型公式为24m 8sm 6st,未发现性染色体和随体.与海螂目另一物种砂海螂相比,二倍体染色体数目多4条,亚端部着丝粒染色体(st)多6条,核型比砂海螂更具不对称,推测宽壳全海笋较砂海螂原始.