Hematological changes in white spot syndrome virus-infected shrimp, Fenneropenaeus chinensis (Osbeck)

The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV (white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifestations of hemocytes were similar among moribund shrimps infected via injection, feeding and by nature. Firstly, the total hemocyte counts (THCs) in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly, necrotic, broken and disintegrated cells were often observed, and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly, necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared, whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV, and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.     

Heavy mineral stratigraphy of sediments from the southern outer shelf of the East China Sea since the last glaciation using fuzzy C-means cluster method

Correspondence analysis and fuzzy C-means cluster methods were used to divide the stratigraphy of heavy mineral assemblages,and the sediment sources and depositional dynamics of the environment reconstructed.The assemblages were taken from marine sediments from the late Pleistocene to the Holocene in Core Q43 situated on the outer shelf of the East China Sea.Based on the variable boundaries of the mineral assemblage at 63 and 228 cmbsf (cm below sea floor),the core might have previously been divided into th...     

三维地籍产权体的定义与表达

传统意义上的地籍是以宗地作为基本单元进行权属登记和管理,以地表权利为核心的二维地籍。而三维地籍同样需要一种类似于二维宗地的基本单元来登记和管理三维空间中的权属状况,这种三维基本单元被称为产权体。本文以土地和房产等权利实体为基础,以权利空间为参考,研究三维空间中土地和房产的划分方式,分析这两种基本单元的存在形式、划分方式和空间形态,概括出它们的本质特征。以这些本质特征为基础,给出产权体定义和表达方式,并验证了所定义产权体的正确性和有效性。     

pCO2 and carbon fluxes across sea-air interface in the Changjiang Estuary and Hangzhou Bay

Partial pressure of CO2(pCO2) was investigated in the Changjiang(Yangtze River) Estuary,Hangzhou Bay and their adjacent areas during a cruise in August 2004,China.The data show that pCO2 in surface waters of the studied area was higher than that in the atmosphere with only exception of a patch east of Zhoushan Archipelago.The pCO2 varied from 168 to 2 264 μatm,which fell in the low range compared with those of other estuaries in the world.The calculated sea-air CO2 fluxes decreased offshore and varied from -10.0 to 88.1 mmol m-2 d-1 in average of 24.4 ± 16.5 mmol m-2 d-1.Although the area studied was estimated only 2 × 104 km2,it emitted(5.9 ± 4.0) × 103 tons of carbon to the atmosphere every day.The estuaries and their plumes must be further studied for better understanding the role of coastal seas playing in the global oceanic carbon cycle.     

罗非鱼4个选育群体遗传结构SSR分析

采用SSR分子标记分析了吉富品系罗非鱼的两个家系(GF1和GF2)以及奥利亚罗非鱼(Fo)和奥本尼罗罗非鱼(Fn)群体的遗传结构。结果显示,扩增后等位基因数为3～8个,随引物不同而异,14对引物共扩增60个等位基因,扩增片断大小在102～267 bp之间。微卫星分析表明,奥本尼罗罗非鱼(Fn)的平均观测杂合度(0.764 3)和平均期望杂合度(0.519 6)均最高,吉富尼罗罗非鱼(GF1)平均多态信息含量(0.419 5)最高;吉富尼罗罗非鱼(GF2)平均观测杂合度(0.614 2),奥利亚罗非鱼(Fo)的平均期望观测度(0.446 6)、平均多态信息含量(0.359 2)最低;因此,吉富鱼(GF1)的遗传多样性最高,奥利亚的遗传多样性最低。Hardy-Weinberg平衡遗传偏离指数(D)奥利亚(Fo)和尼罗(Fn)(0.463 4和0.478 9)明显高于吉富的两家系(0.234 1和0.250 0)。Fo与GF2间遗传距离(0.477 0)最大;而GF1和GF2间的遗传距离(0.302 7)最小,遗传相似系数(0.607 3)最大,可推断新一代吉富罗非鱼与本地选育群体有相对较远的亲缘关系,更具杂种优势。     

波纹开缝翅片管换热器传热与流动性的数值模拟

提出一种波纹开缝翅片管换热器,对其传热与流动特性进行三维数值模拟,选择Re范围在1 000到5 000时与平板开缝翅片的模拟相比较。结果表明,在Re小于2 752时,平板开缝比波纹开缝的换热效果好,而Re大于2 752时,波纹开缝比平板开缝换热效果更好,并且波纹开缝翅片管换热器的流动阻力小于平板开缝,因此,Re大于2 752时波纹开缝比平板开缝综合效果更好。     

基于Struts的在线考试系统的设计与实现

介绍了如何采用Struts框架技术结合UML设计和实现一个在线考试系统。给出了系统的需求分析,用例模型及软件结构图,主要功能模块的MVC框架设计,介绍了一些关键问题的解决方案和实现代码。     

万福矿井深部人工冻结粘土层的力学性状

根据万福矿井400m以下巨厚粘性土层取样进行的冻土力学性状试验结果,深部冻结粘性土的应力应变关系主要表现为应变软化特点,达到峰值应力之前显现较大幅度的屈服变形。从冻结效果看,-20℃是使深部粘性土力学性状发生明显变化的冻结状态界限值,冻结粘土样的强度明显提高,而蠕变性则明显降低,达到-20℃后,随着温度进一步降低,强度和蠕变性的变化则逐渐趋缓。     

准噶尔盆地中部Ⅲ区块原油甾烷异常分布特征与成因

对准噶尔盆地中部Ⅲ区块的3类原油或油砂样品抽提物饱和烃进行了气相色谱-质谱分析,研究了原油饱和烃中甾烷类化合物的分布特征,并对其成因做了初步探讨。结果表明：3类原油的甾烷分布特征具有一定的相似性,C27规则甾烷相对丰度明显低于C28、C29;C27、C28、C29异构体αββ型相对丰度明显高于ααα型（C28αββ（20S）或C29αββ（20R）丰度最高）;但这3类原油的成因是不一致的,其影响因素主要包括热演化、生物降解、运移分馏以及古环境或岩性4个方面,其中第1、3类原油主要以热演化作用与运移作用为主,第1类原油还可能受到古环境或岩性影响,两者均不受生物降解的影响,第2类原油则受到热演化、运移作用及生物降解的共同影响。     

基于MapObjects的闽江口湿地信息系统

在动态度、马尔柯夫链等数学方法和景观生态学理论指导下,建立了闽江口湿地时空演变模型,应用组件式技术,利用MapObjects在Visual Basic平台上,进行闽江口湿地时空演变信息系统设计和开发,并应用该信息系统对闽江口湿地时空演变进行实例分析。结果表明,该系统能更高效、直观管理闽江口湿地空间信息和属性信息,以形象化的方式对湿地作全局性时空分析,使得闽江口湿地管理和保护更加科学。