Isolation and characterization of 49 polymorphic microsatellite loci for Decapterus maruadsi using SLAF-seq,and cross-amplification to related species

Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( H_o ) and expected heterozygosity ( H_e ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.

     

生态系统服务弹性敏感性系数的合理性与决策属性探讨

在经济学弹性基本概念的基础之上,采用数学推导的方式重点探讨3种生态系统服务弹性敏感性评价模型的合理性与决策属性。研究结果表明：① Kreuter敏感性系数大小始终为0~1;在极限形式下,生态系统服务价值变率函数与Kreuter敏感性系数具有相同的数学表达式与值域;所以这两种敏感性评价模型把1作为是否敏感的评价标准并不合适。生态系统服务交叉敏感性系数不符合一般意义上的“交叉敏感性”的概念,并且其计算公式不符合弹性的基本定义。② 弹性敏感性计算方式适用于随机变量间的研究,不适用于具有确定性关系的变量;生态系统服务框架下的3种弹性敏感性系数均建立在具有确定性关系的生态系统服务价值计算公式的基础之上,导致其敏感性计算结果缺乏深层次的决策属性。     

广东雷州珍稀海洋生物国家级自然保护区 海水环境质量状况分析与评价

2018年9月和12月,两次调查了广东雷州珍稀海洋生物国家级自然保护区海域海水的环境质量。结果表明,该保护区平均综合水质指数为0.38,一类海水平均达标率为93.59%,海水中铅、磷酸盐、锌的含量达标率偏低,分别为53.85%、73.1%、96.15%。12月保护区海水中的无机氮、磷酸盐、溶解氧、叶绿素a含量明显高于9月,保护区实验区和缓冲区站位的水质优于核心区站位的水质。该保护区海域综合水质定性评价结果为优。靠近康港附近站位的营养盐相对偏高,磷酸盐的平均达标率仅为73.1%,因此,需加强对康港附近海域养殖情况的监控,尽量减少养殖废水对保护区海域的排放,保持保护区海水清洁,以利于对保护区内珍稀海洋生物及其栖息地的保护。     

海表面温度时空变异特征及对验证误差影响

海洋要素的变化存在明显的区域性和季节性的变化特性,本文选择海洋要素中最为突出的海表面温度(SST)要素作为主要分析参数,设计时空变异参数的计算指标,分析时空变异对验证误差影响的关系,通过研究及试验的数据精度验证,证明了时空变异是造成误差的直接原因之一。强烈的时空属性变异,在验证过程中会引入很大的验证误差,处于不同变异等级区划的数据,其验证结果相对误差可达13.08%,变异越剧烈的区域,精度验证效果越差,验证误差就越大,这些误差并非完全是遥感产品的误差,验证结果不具有代表性,不能真实的反映遥感产品的误差特征。对于SST等海洋遥感产品验证时,需要考虑时空变异对验证误差的影响和贡献,合理选择验证试验区域、代表性的评价数据集和科学的评价方法。     

空间分辨率对中国夏季降水时空演变特征的影响

本文使用1979—2021年国家气候中心160站(R₁₆₀)和国家级地面气象观测站2 314站(R₂₃₁₄)逐月降水观测资料,利用EOF (Empirical Orthogonal Function)分解和相关分析等方法,研究两类资料夏季降水时空变化特征及其与海气因素之间物理联系的表征水平差异,并分析了差异的可能原因。结果表明,R₁₆₀在我国西北、青藏高原等地区站点极为稀疏,导致其各EOF模态对上述地区降水的时空变化特征描述失真,中东部地区偏低的空间分辨率会使局地强降水的变化特征信号损失,造成降水的年际变率降低。而R₂₃₁₄主模态能够更为真实地反映我国降水的时空演变特征,特别是在极端降水频发的江淮地区以及降水受局地地形影响较大的山区,其EOF主模态的空间分布和时间系数演变与ENSO (El Niño-Southern Oscillation)、西太平洋副热带高压、青藏高原雪盖等气候强迫信号之间的相关性更为显著。     

中国北方磷矿矿床类型及成矿规律

通过对中国北方内生磷灰石、变质及沉积型磷矿成矿特征及典型矿床的研究,建立了各种类型磷矿床的成矿模式和找矿模型、确定了各类型矿床的时空分布规律以及矿床的共生规律,为开展磷矿的找矿工作具有重要参考价值。     

中国北方磷矿成矿预测

在中朝准地台、塔里木准地台边缘深断裂附近和台中隆起带以及天山-兴安、祁连-秦岭、额尔古纳几个地槽褶皱区的中间地带,不同程度地具备中国北方三种主要类型磷矿的成矿条件。研究分析认为中国北方的内生磷矿具有良好的找矿前景,绿岩带型磷矿和古元古代沉积变质磷矿显示一定的找矿潜力,沉积型磷矿一般品位低、规模小。通过归纳,在中国北方划分出磷矿的二级城矿区4个、三级成矿带15个、四级成矿亚带19个。圈定成矿预测区A级2个,B级C级各23个。确定了21处可开展进一步工作的地区。     

Formation and early development of tetraspores of Polysiphonia urceolata （Rhodomelaceae, Rhodophyta）

Polysiphonia urceolata is one type of potential commercial red seaweeds used for breeding and cultivation, because of its significant biochemical and biomedical application. However, the information of breeding and seedling incubation for cultivation is limited, especially the early development. In this study, tetrasporohyte and gametophyte of P. urceolata were taken as the study materials in Huiquan Bay, Qingdao, China. The cleaned and sterilized tetrasporophytes and gametophytes were pre-cultured in sterilized seawater, then nurtured at 18°C, 25 μmol photons m⁻² s⁻¹ in 12:12 h (light:dark) photoperiod. Continuous observation under microscope showed that the early development consists of bipolar division stage and seedling stage. In the division stage, tetraspores germinate into bipolar sporelings that further differentiate into a colorless rhizoidal portion and a lightly pigmented upright shoot. The lightly pigmented rhizoidal cell develops to a rhizoid and the larger pigmented cell transforms to an erect axis. In the seedling stage, several quasi-protuberances appear on the erect axis and form juvenile seedlings. The results demonstrate the culture of P. urceolata from tetraspores under laboratory conditions. Supported by National Key Technology Support Program, Development Program of China (No.2006AA09Z21), National Natural Science Foundation of China (No. 40618001 and N_CUHK438/06) and Shandong Agricultural Seed Stock Breeding Project     

拮抗香蕉枯萎病菌海洋细菌的筛选和鉴定

从93份中国南部海域近海海泥样品中分离出1800多株海洋细菌,以对峙培养法,测定海洋细菌对香蕉枯萎菌的拮抗作用,再以琼脂划线培养法(agar streak)测定菌株的抑菌活性,结果表明:约55.6%的菌株对香蕉枯萎菌有一定的拮抗作用,其中58株菌株抑制香蕉枯萎菌生长所形成的抑菌带宽度大于10.0mm,抑菌作用较好,复筛后其中13个菌株形成的抑菌带宽在15.0mm以上。经反复转接和对峙培养,筛选出拮抗效果好、抑菌活性稳定的菌株TC-1,初步鉴定为芽孢杆菌属的细菌,TC-1对14种病原微生物都有较好的抑菌效果。     

北黄海温盐分布季节变化特征分析

利用2006～2007年夏冬春秋4个季节北黄海的大面调查资料,分析了4个季节北黄海温度和盐度大面以及典型断面分布特征,得出以下结论:2007年冷水团势力范围强于2006年,北黄海冷水团的形成受地形影响.黄海暖流冬春季较强,冬季最强,夏季最弱,秋季开始形成.鲁北沿岸流冬季最强,春季减弱,夏秋季消失,但夏季鲁北沿岸存在冬季鲁北沿岸流水的残余体,即鲁北沿岸水.辽南沿岸水4个季节都以低盐为特征,除夏季低盐中心位于庄河口外,其它3个季节低盐中心均位于调查区域的东北角.渤海与北黄海之间的水交换4个季节都存在.春季,断面盐跃层形成滞后于温跃层;秋季,断面盐跃层消失滞后于温跃层.