Nitrate assimilation by zooxanthellae maintained in laboratory culture

The ability of cultured zooxanthellae to use ammonium and nitrate was tested. The zooxanthellae were initially isolated from the anemone Aiptasia pulchella. The zooxanthellae were able to grow on media supplemented with either form of dissolved inorganic nitrogen (DIN) although ammonium was inhibitory above 300 μM. In accord with the intact symbiosis and freshly isolated algae, the cultured zooxanthellae took up ammonium. In contrast, the cultured algae demonstrated nitrate uptake and utilization. This was enhanced with DIN deprivation. Nitrate reductase was found in the algae and appeared to be inducible. Within the animal host, excretory ammonium may repress this induction.     

A general method for calculating three-dimensional laminar and turbulent boundary layers on ship hulls

A general method for representing the flow properties in the three-dimensional boundary layers around ship hulls of arbitrary shape is described. It makes use of an efficient two-point finite-diffirence schem to solve the boundary-layer equations and includes an algebraic eddy-viscosity representaion of the Reynolds-stress ternsor. The numericzal method contains novel and desirable features and allows the calculation of flows in which the circumferential velocity component contains regions of flow reversal across the boundary layer. The inviscid pressure distribution is determined with the Douglas-Neumann method which, if necessary, can conveniently allow for the boundary-layer displacement surface. To allow its application to ships, and particularly to those with double-elliptic and flat-bottomed hulls, a non-orthogonal coordinate system has been developed and is shown to be economical, precise and comparatively easy to use. Present calculations relate to zero Froude number but they can be extended to include the effects of a water wave and local regions of flow separation which may stem from bulbous-bow geometries.     

Secondary flow caused by differential roughness,Langmuir circulations,and their effect on the development of sand ribbons

The process of Differential Roughness Secondary Flow (DRSF), hitherto little recognized, recently was demonstrated in a wind tunnel and in a flume. It is suggested here the DRSF may, in the natural environment, play an important part in the persistence and sharp definition of sand ribbons, previously initiated by other processes. In 1980 Karl described a series of sand ribbons, alternating with exposed substrate, which he attributed to the effect of Langmuir circulations. Although Langmuir Circulation Secondary Flow (LCSF) may indeed, in some cases, initiate sand ribbons, the sharply-defined edges typical of these features are explained better by DRSF.     

Palaeo-carbonate seep structures above an oil reservoir, Gryphon Field, Tertiary, North Sea

Petrographic and geochemical analyses performed on a North Sea core from the Gryphon Field reveal the presence of palaeo-degassing features surrounded by injected sandstones in the Eocene interval. The injected sandstones are oil-stained and poorly cemented by carbonate and quartz. ¹⁸O isotope analyses indicate that carbonate cementation occurred during shallow burial (likely less than about 300 m). Depleted ¹³C (around –30 V-PDB) carbonate cement suggests that bicarbonate was derived from the microbial oxidation of oil and gas. Late quartz overgrowths enclose oil present in the injected units. The tubular degassing conduits are composed of zoned cements and have ¹⁸O and ¹³C isotope values similar to the injected sandstones, indicating that oil and gas seepage induced the precipitation of authigenic carbonate in the shallow subsurface. Oil inclusions in inter- and intra-crystal cement sites in both injected sandstones and degassing conduits indicate that oil seepage was an ongoing feature at shallow burial. A proposed model involves oil and gas seepage and the formation of the degassing conduits, followed by a sand injection phase. It seems likely that oil and gas continued to leak towards the seabed by exploiting the network of permeable injected sandstones and the horizons of porous degassing features.     

New seismic data in the Mozambique Channel

This paper presents seismic reflection and refraction data from the Mozambique Channel, collected between 1971 and 1973. A deep sedimentary basin (up to 5 km of sediments) opens southwards to the Mozambique Basin, and is bounded to the east by the Davie Ridge and beyond by the marginal plateau of Malagasy. A continuous reflector (C), possibly of Cretaceous age, is identified between layers having seismic interval velocities of 2.4–2.8 km/s and 3.1–3.4 km/s. The deepest sediments have velocities of 4.5–4.9 km/s and overlie a layer with velocity 5.5 km/s, which may be volcanic in the north-east of the Channel.The crust occupying most of the Channel is probably pre-Cretaceous in age, and may be largely continental in nature. This is supported by subdued magnetic anomalies and the possibility of a continuous Karroo sedimentary section across the northern Channel. The oceanic crust of the Mozambique Basin may extend as far north as 24°S, into the western Channel only. The problem of the origin of the Mozambique Channel remains unresolved, although a long sedimentary history indicates that Malagasy may have separated from Mainland Africa prior to Karroo times. The Davie Ridge may possibly represent a relict strike-slip fault, which permitted movement along a north-south line.     

Primary production and plankton stocks in the Pacific Ocean and their seasonal variation according to remote sensing and field observations

Data collected from 20 years of observations by the P. P. Shirshov Institute of Oceanology (about 250 comprehensive ecosystem stations) are summarized to evaluate the primary production and biomass of the principal groups of plankton in the Pacific Ocean. The stations were classified into geographical regions according to satellite determined criteria. The areas of these regions were evaluated according to CZCS data from 1978 to 1986. The total value of primary production was evaluated as 26·9 Gt C year⁻¹. Taking the “bottle effect” correction into account, this value may be as high as 45.6 Gt C year⁻¹. Total biomass values of phytoplankton (134 Mt C), bacteria (86 Mt C), protozoa (26 Mt C) and mesoplankton (184 Mt C) were also calculated. Seasonal variabilities of all these values were remarkably insignificant.     

Vertical and lateral transport of organic carbon and the carbon budget in Santa Monica Basin, California

The flux and compositions of solvent-extractable lipid fractions were measured in particulate matter collected periodically by moored sediment traps in the Santa Monica Basin from 1985–1988. The purpose was to assess the compositional changes during settling, the carbon dynamics in the basin and to estimate the impacts of energy-related by-products on the surface sediments. Sediment traps recorded consistently high lipid fluxes in the eastern slope relative to the central basin, reflecting elevated terrigenous carbon inputs possibly from land-based human activities. Generally, lipid fluxes decrease offshore but increase vertically with water depth below 500m, implying lateral transport of particles. The steep decline of flux in the top 500m of the water is related to the rapid decomposition and mineralization of the marine-derived cellular carbon compounds. Less than 5% of the marine lipid components reach the seabed. In contrast, preferential preservation of terrigenous lipid is clearly evident from the chemistry of deeper traps and surface sediments.The lateral transport of particles is reflected in the presence of higher plant-derived lignin phenols and sewage-derived coprostanol and epicoprostanol in the deep trap material as well as in surface sediment throughout the basin. Petroleum triterpanes characteristic of natural seepage also permeates through the entire basin. Based on the data collected from both the trap particulte matter and surface sediments, a carbon budget for the Santa Monica Basin has been constructed.