全文获取类型
收费全文 | 5910篇 |
免费 | 1211篇 |
国内免费 | 1711篇 |
专业分类
测绘学 | 442篇 |
大气科学 | 1387篇 |
地球物理 | 1469篇 |
地质学 | 3208篇 |
海洋学 | 623篇 |
天文学 | 278篇 |
综合类 | 722篇 |
自然地理 | 703篇 |
出版年
2024年 | 30篇 |
2023年 | 120篇 |
2022年 | 274篇 |
2021年 | 349篇 |
2020年 | 269篇 |
2019年 | 281篇 |
2018年 | 392篇 |
2017年 | 324篇 |
2016年 | 370篇 |
2015年 | 336篇 |
2014年 | 355篇 |
2013年 | 370篇 |
2012年 | 317篇 |
2011年 | 335篇 |
2010年 | 398篇 |
2009年 | 349篇 |
2008年 | 317篇 |
2007年 | 331篇 |
2006年 | 248篇 |
2005年 | 239篇 |
2004年 | 189篇 |
2003年 | 173篇 |
2002年 | 151篇 |
2001年 | 181篇 |
2000年 | 194篇 |
1999年 | 255篇 |
1998年 | 249篇 |
1997年 | 236篇 |
1996年 | 210篇 |
1995年 | 157篇 |
1994年 | 152篇 |
1993年 | 144篇 |
1992年 | 113篇 |
1991年 | 85篇 |
1990年 | 62篇 |
1989年 | 65篇 |
1988年 | 60篇 |
1987年 | 31篇 |
1986年 | 32篇 |
1985年 | 18篇 |
1984年 | 14篇 |
1983年 | 14篇 |
1982年 | 9篇 |
1981年 | 9篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1975年 | 5篇 |
1960年 | 2篇 |
1959年 | 1篇 |
1958年 | 7篇 |
排序方式: 共有8832条查询结果,搜索用时 125 毫秒
311.
Guoyan Ren Bafang Li Xue Zhao Yongliang Zhuang Mingyan Yan Hu Hou Xiukun Zhang Li Chen 《中国海洋大学学报(英文版)》2009,8(1):83-88
In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified
target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted
against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions
containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted
water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q
water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method
was the optimum extraction method selected by HPLC. 相似文献
312.
Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes. 相似文献
313.
平阴县土壤中重金属元素异常成因研究 总被引:1,自引:0,他引:1
通过对平阴县周边及其西部农田区采样调查,平阴县周边元素异常具有异常衬度较高,富集系数大等特征,西部农田区异常具有各元素套合关系好,多数异常衬度不高,富集系数小的特点。结果表明,西部农田区异常是以As,Cd为主要成分的异常,元素含量与SiO2,Al2O3,Fe2O3等常量组分间具有显著的相关性,随SiO2含量的增加而降低,随Al2O3,Fe2O3含量的增加而增加,说明土壤常量组分及其所决定的土壤质地是导致异常形成的主要因素;而平阴城区异常是以S,Hg,As,Zn,Mn等元素为主的异常,是典型的人为综合因素(化工烟尘、工厂污水、生活垃圾)产生的异常。 相似文献
314.
315.
316.
三维城市模型的研究与实践(英文) 总被引:3,自引:0,他引:3
The way we interact with spatial data has been changed from 2D map to 3D Virtual Geographic Environment (VGE). Three-dimensional
representations of geographic information on a computer are known as VGE, and in particular 3D city models provide an efficient
way to integrate massive, heterogenous geospatial information and georeferenced information in urban areas. 3D city modeling
(3DCM) is an active research and practice topic in distinct application areas. This paper introduces different modeling paradigms
employed in 3D GIS, virtual environment, and AEC/FM. Up-to-date 3DCM technologies are evolving into a data integration and
collaborative approach to represent the full spatial coverage of a city, to model both aboveground and underground, outdoor
and indoor environments including man-made objects and natural features with 3D geometry, appearance, topology and semantics.
Supported by the National Natural Science Foundation of China ( No. 40871212, No. 40671158), the Leading Academic Discipline
Project of Shanghai Educational Committee( No.J50104). 相似文献
317.
WANG Hu ZHOU Huaiyang PENG Xiaotong YANG Qunhui QIN Chaomei YIN Xijie CHEN Guangqian 《海洋学报(英文版)》2009,28(1):37-46
Samples of sediments and the overlying water were collected in the Qi'ao Island coastal zone, the Zhujiang (Pearl River) Estuary (ZE). Denitrification rates, sediment oxygen demand (SOD) , and fluxes of inorganic nitrogen compounds were investigated with N2 flux method, using a self-designed continuous flow through and auto-sampling system. The results indicate that the denitrification rates varied between 222 and 908 μmol/(m2·h) with an average of 499 μmol/(m2·h). During incubation, the sediments absorbed dissolved oxygen in the overlying water with SOD ranging from 300 to 2 363 μmol/(m2·h). The denitrification rates were highly correlated with the SOD (r2 =0.77) regardless of the NO3- + NO2- concentrations in the overlying water, organ- ic carbon contents in sediments and water temperature, suggesting that the SOD was probably the main environ-mental factor controlling the denitrification in the Qi'ao Island coastal zone. There was a net flux of NO3- + NO2-into the sediments from the overlying water. The NH4+ flux from sediments into water as the result of mineraliza-tion was between 12. 3 and 210. 3 μmol/(m2·h) ,which seems limited by both organic carbon content in sedi-ment and dissolved oxygen concentration in the overlying water. 相似文献
318.
�������Ƶ���¼���� 总被引:1,自引:0,他引:1
????????????????????????????????????????????????????????????????????????????????????????????????????У?1??????????????????????e -1.5????????????(<20 km)?????(>20 km)?????????????;2????????????????????????????????????????1?????????????????Ч????????У???????Щ???????????ε???Ч???3???????S???????????????????????P???????С,?????????????????в???????й?? 相似文献
319.
???????λ????????????2004??1???2007??5??ML??1.5????????????ж?λ??????????2004??9??17?????????4.9????????????????????????????????????????ML??2.0?????????????б?????????????4.9???????????????????????????λ???????£????????1?±?????????????????????????????????4.9????????????????????? 相似文献
320.
Tidal current observation in the southern Yellow Sea in the summers of 2001 and 2003 总被引:1,自引:0,他引:1
Direct current observations in the Yellow Sea interior are very scarce due to intense fishing and trawling activities. Most previous studies on tides in the area were based on coastal measurements or satellite altimeter sea levels and have not been rigorously compared with direct measurements. In this paper, tidal currents are studied with current profiles from three bottom-moored Sontek Acoustic Doppler Profilers (ADPs) deployed in the southern Yellow Sea in summer of 2001 and 2003. The measured current series were dominated by tidal currents. Maximum velocities are between 40-80 cm/s at the mooring stations. M2 current is the most dominant primary tidal constituent, while MS4 and M4 are the most significant shallow water tides with much smaller amplitudes than the primary tides. 相似文献