冷空气引起的飞船主着陆场区偏北大风的特征分析及预报

分析了飞船主着陆场区1981—1999年1—4月和9—12月因冷空气而产生的偏北大风的过程中气候概况及其天气特征。通过分析发现:主着陆场区因冷空气引起的偏北大风以春季最频繁,秋、冬季最稀少;一天当中偏北大风以午后出现频率最大,午夜出现频率最少;对于不同季节的气压和温度,春、秋两季变化剧烈,冬季相对较小;在大风出现前24小时,主着陆场区的欧亚中高纬度大气环流以两槽一脊型、一槽一脊型、贝湖低压型为主;冷空气入侵前24小时,欧亚天气图上主着陆场区上游低层850hPa中高纬度有明显的冷中心,地面图场区上游冷高压中心的分布主要有3个区域:贝加尔湖西南至新疆、贝加尔湖到内蒙中北部、贝加尔湖西北部。大风前24小时在35～45°N、100～115°E等压线密集,等压线一般都在4根以上。     

Feasibility of Re-injecting the Production Effluent from Alkali–Surfactant–Polymer Flooding Systems Back into a Reservoir to Further Improve Oil Recovery

Natural Resources Research - Alkali–surfactant–polymer (ASP)-produced effluent contains polymer, alkali and surfactant, and it has higher content of suspended oil droplets and suspended...     

Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.     

Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.     

Co-dwelling,Mix-dwelling and Dis-dwelling: The Diversity Among Three Human and Livestock Dwelling Forms in Rural China

Humans and livestock have always dwelling together, especially in rural areas. Based on a multi-method approach of ethnographic fieldwork, grounded theory and one-way multivariate analysis of variance, this paper explores human and livestock dwelling. The findings show that human and livestock dwelling form a special type of human dwelling place, with forms defined as co-, mix- and dis-dwelling. Then, from co-, mix- to dis-dwelling constructed a story line to the evolution of human and livestock dwelling forms, which further explains the entire dwelling situation of humans and livestock, including the dimensions of human dweller, livestock dweller, the rural environment and special events. Among co-, mix- and dis-dwelling significant differences existed between human and livestock individuals, the nature of rural environment, vernacularity and rural tourism development. Between co- and dis-dwelling, significant differences existed in seventeen aspects; between mix- and dis-dwelling, significant differences existed in eleven aspects; meanwhile, no significant differences existed between co- and mix-dwelling. Accordingly, this suggested that human and livestock dwelling forms can be divided into mix- and dis-dwelling. This study on humans and livestock dwelling relationship offer a new perspective on rural and animal geography. This paper is an exploratory foray into rural dwelling geography as part of the Anthrozoology study and broadens the scope of extant scholarship on human dwelling morphology, as well as analyses the spatial relationships between human and livestock dwelling.     

柴达木盆地东北缘中中新世以来古气候变化

全球变冷、青藏高原隆升以及特提斯洋海退被认为是控制亚洲大陆新生代气候变化的三大因素,然而由于长时间尺度古气候记录的匮乏,对于三者影响的认识并不清晰。柴达木盆地是青藏高原北部最大的一个封闭盆地,发育巨厚且连续的新生代地层,详细记录了周缘山体构造隆升过程和区域气候演化历史。本文通过柴达木盆地东北缘怀头他拉剖面的中中新世-早更新世沉积物气候代用指标碳酸盐、硫酸根和氯离子含量分析,显示该研究区中中新世以来气候变化经历了15.3~13.0Ma相对湿润、13.0~6.6Ma半干旱-半湿润和6.6~1.8Ma干旱的3个演化阶段。结合生物特征和前人研究成果,认为柴达木盆地干旱化历史可能从中中新世13Ma左右开始逐步发展,而真正意义上的干旱化始于6.6Ma以来。全球变冷是控制柴达木盆地干旱气候形成和发展的主导因素,同时青藏高原晚期持续阶段性的强烈隆升起到了催化剂的作用。     

三角剪切断层传播褶皱在祁连山北缘断裂中的应用:以黑河口断层为例

逆冲断层运动学过程研究可为造山作用和盆地演化提供重要的信息。位于青藏高原东北缘的祁连山北缘逆冲断裂（NFT）是一条主要的边界断裂,控制着祁连山晚新生代以来的隆升变形,但对于该断裂系统的运动学过程缺乏研究。甘肃张掖西南莺落峡附近,黑河口断层（祁连山北缘断裂的一部分）穿过红沙沟并导致其阶地面错断和褶皱变形。为了研究该区域褶皱变形特征和断层运动学过程,我们首先在红沙沟区域进行了详细的野外考察并对变形的地层和阶地面进行了测量,然后通过数值模拟方法来验证褶皱模型。红沙沟保存有9级河流阶地,利用差分GPS对阶地面的高程进行了测量,同时在野外测量了部分地层的产状。多处断层剖面表明该区域变形属于基底卷入的构造类型,另外变形的阶地面在近断层处表现出明显的褶皱形态。结合阶地面变形特征以及地层的变形,三角剪切断层传播褶皱模型被初步用于该区域构造变形的解释。模型模拟得到自T5、T6和T7形成以来断层滑动量分别是 44±7m、59±10m和 164±28m;结合T6年代得到自约9万年以来该断层的平均滑动速率为 0.68±0.19mm/a,垂直分量为 0.58±0.13mm/a。根据地层与阶地变形特征,并结合模型模拟结果,我们认为祁连山山前基底断层卷入的构造中,在断层出露地表之前,断层顶端存在一个分散式的三角形变形区,区域的应力状态控制着下伏地层和上覆地貌面的褶皱变形。     

黄斑篮子鱼去毒相关基因的克隆与肝脏组成型表达分析

从基因水平探讨海洋鱼类对海洋藻毒素的去毒分子机理。采用RT-PCR法成功克隆了黄斑篮子鱼Siganus oramin肝脏I时相代谢酶细胞色素P450 1A(CYP1A)、II时相代谢酶alpha型谷胱甘肽S-转移酶(GSTA)和rho型谷胱甘肽S-转移酶(GSTR)、热休克蛋白70 (HSP70)、alpha 1型钠钾ATP酶(ATP1A1)及β-肌动蛋白(beta-actin, ACT)基因cDNA核心序列,序列分别长879 bp、582 bp、588 bp、660 bp、749 bp和554 bp。序列同源性分析发现,属鲈形目的黄斑篮子鱼CYP1A、GSTA和GSTR与同属鲈形目的牙鲆Paralichthys olivaceus、欧洲鲽Pleuronectes platessa、真鲷Pagrus major、鲤形目的斑马鱼Brachydanio rerio 相应氨基酸序列同源性较高,CYP1A和GSTA与非洲爪蟾(两栖类)、鸡(鸟类)、小鼠、大鼠和人(哺乳类)相应氨基酸序列同源性低,这可能与鱼类I、II时相去毒酶基因承担水环境毒素去毒代谢的特殊功能有关;而HSP70、ATP1A和β-肌动蛋白在鱼类、两栖类、鸟类、哺乳类中均有较高的同源性,这可能与这些基因在机体中承担的最基本的生命功能相关。应用半定量RT-PCR的方法,以β-肌动蛋白作为外参照,在指数期增长范围内分别得到了CYP1A、GSTA、GSTR、HSP70和ATP1A1 mRNA与β-肌动蛋白mRNA (%)的比值,确定黄斑篮子鱼肝脏去毒相关基因的组成型表达水平。其中,黄斑篮子鱼肝脏CYP1A、GSTA和GSTR基因组成型表达相对较高,HSP70和ATP1A1基因组成型表达相对较低,这可能与不同基因在黄斑篮子鱼海洋藻毒素去毒分子机理中承担的作用相关,为海洋藻毒素在海洋鱼类中的积聚及代谢去毒分子机制的研究提供了相关数据。     

The spatial distribution of archaeal lipids in a mesoscale subtropical watershed,Southeast China

Archaea play an important role in global carbon and nitrogen cycles. Archaeal lipids, such as isoprenoid glycerol diakyl glycerol tetraethers (iGDGTs), are important biomarkers tracing change in archaeal community structure and biogeochemical processes in the natural environments. In this research, the spatial distributions of archaeal lipids in the surface sediments of the Jiulong River (JR) and the Jiulong River estuary (JRE) were examined. GDGT-0 (containing zero cyclopentyl ring) and crenarchaeol were the most abundant iGDGTs in the JR and JRE. From the rivers to the estuary, the total iGDGTs, GDGT-0, crenarchaeol and archaeol concentrations showed significant spatial variation; in particular, GDGT-0 and archaeol in the river may be predominantly derived in situ from methanogens, whereas crenarchaeol in the estuary mainly derived in situ from Thaumarchaeota. We inferred that archaeal community was dominated by methanogens in the Jiulong River and by Thaumarchaeota in the Jiulong River estuary, which are consistent with change in archaeal community structure observed in other estuarine environments.     

A Model for Structure Formation Seeded by Gravitationally Produced Matter

The Arecibo H i Strip Survey probed the halos of approximately 300 cataloged galaxies and the environments of approximately 14 groups with sensitivity to neutral hydrogen masses >/=107 M middle dot in circle. The survey detected no objects with properties resembling the high-velocity clouds (HVCs) associated with the Milky Way or Local Group. If the HVCs were typically MHi=107.5 M middle dot in circle objects distributed throughout groups and galaxy halos at distances of approximately 1 Mpc, the survey should have made approximately 70 HVC detections in groups and approximately 250 detections around galaxies. The null detection implies that HVCs are deployed at typical distances of 相似文献