Benthic photosynthesis in submerged Wadden Sea intertidal flats

In this study we compare benthic photosynthesis during inundation in coarse sand, fine sand, and mixed sediment (sand/mud) intertidal flats in the German Wadden Sea. In situ determinations of oxygen-, DIC- and nutrient fluxes in stirred benthic chamber incubations were combined with measurements of sedimentary chlorophyll, incident light intensity at the sediment surface and scalar irradiance within the sediment. During submergence, microphytobenthos was light limited at all study sites as indicated by rapid response of gross photosynthesis to increasing incident light at the sea floor. However, depth integrated scalar irradiance was 2 to 3 times higher in the sands than in the mud. Consequently, gross photosynthesis in the net autotrophic fine sand and coarse sand flats during inundation was on average 4 and 11 times higher than in the net heterotrophic mud flat, despite higher total chlorophyll concentration in mud. Benthic photosynthesis may be enhanced in intertidal sands during inundation due to: (1) higher light availability to the microphytobenthos in the sands compared to muds, (2) more efficient transport of photosynthesis-limiting solutes to the microalgae with pore water flows in the permeable sands, and (3) more active metabolic state and different life strategies of microphytobenthos inhabiting sands.     

Phytoplankton composition and biomass across the southern Indian Ocean

Phytoplankton composition and biomass was investigated across the southern Indian Ocean. Phytoplankton composition was determined from pigment analysis with subsequent calculations of group contributions to total chlorophyll a (Chl a) using CHEMTAX and, in addition, by examination in the microscope. The different plankton communities detected reflected the different water masses along a transect from Cape Town, South Africa, to Broome, Australia. The first station was influenced by the Agulhas Current with a very deep mixed surface layer. Based on pigment analysis this station was dominated by haptophytes, pelagophytes, cyanobacteria, and prasinophytes. Sub-Antarctic waters of the Southern Ocean were encountered at the next station, where new nutrients were intruded to the surface layer and the total Chl a concentration reached high concentrations of 1.7 ??g Chl a L⁻¹ with increased proportions of diatoms and dinoflagellates. The third station was also influenced by Southern Ocean waters, but located in a transition area on the boundary to subtropical water. Prochlorophytes appeared in the samples and Chl a was low, i.e., 0.3 ??g L⁻¹ in the surface with prevalence of haptophytes, pelagophytes, and cyanobacteria. The next two stations were located in the subtropical gyre with little mixing and general oligotrophic conditions where prochlorophytes, haptophytes and pelagophytes dominated. The last two stations were located in tropical waters influenced by down-welling of the Leeuwin Current and particularly prochlorophytes dominated at these two stations, but also pelagophytes, haptophytes and cyanobacteria were abundant. Haptophytes Type 6 (sensuZapata et al., 2004), most likely Emiliania huxleyi, and pelagophytes were the dominating eucaryotes in the southern Indian Ocean. Prochlorophytes dominated in the subtrophic and oligotrophic eastern Indian Ocean where Chl a was low, i.e., 0.043-0.086 ??g total Chl a L⁻¹ in the surface, and up to 0.4 ??g Chl a L⁻¹ at deep Chl a maximum. From the pigment analyses it was found that the dinoflagellates of unknown trophy enumerated in the microscope at the oligotrophic stations were possibly heterotrophic or mixotrophic. Presence of zeaxanthin containing heterotrophic bacteria may have increased the abundance of cyanobacteria determined by CHEMTAX.     

Lysosomal injury and MFO activity in the liver of flounder (Platichthys flesus L.) in relation to histopathology of hepatic degeneration and carcinogenesis

Flounder (Platichthys flesus L.), 18–48 cm total length were sampled in the mouth of the Elbe River during a 3-year interdisciplinary project ‘Fish Diseases in the Wadden Sea’. Lysosomal changes (lysosomal membrane stability) and the activity of biotransforming enzymes (MFOs/EROD) were measured parallel to investigations of ultrastructural changes in liver cells, tissue pathologies and macroscopically visible changes. The aim of the study was to investigate if these cyto- and biochemical parameters were able to reflect contaminant induced biological effects. Interlinking of the results of the MFO activity to the pathological alterations observed at the electron- and light-microscopic levels as well as during macroscopic inspection of identical individuals evidenced that the activity of the biotransformation enzymes (EROD) was not induced in healthy livers, increased considerably with the onset of liver changes and dropped again in those livers with degenerative, preneoplastic and neoplastic lesions. In contrast, concurrent studies of lysosomal membrane stability measured in parallel showed a decrease with the onset and progression of liver lesions from reversible to irreversible, neoplastic change.     

After UNCLOS: the future of international marine research

The April 1984 special issue of Marine Policy included an overview article on international marine research in the post-UNCLOS era: ‘Marine science: organizing the study of the oceans’ by Henry Charnock. We sent a galley proof of the article to Dr Hans Tambs-Lyche, formerly of ICES, for comment and we are pleased to publish his remarks.     

Econometric analysis to differentiate effects of various ship safety inspections

This article provides a refined technique to measure and interpret variables associated with the quality of an inspection—be it port state control or vetting inspections towards the probability of a very serious, serious or less serious casualty. It concentrates on filtering out the effect of variables such as detention, the port state control regime that inspected the vessel, time in-between inspections, deficiencies found during an inspection and the effect of vetting inspections. The consensus amongst policy makers in the shipping industry is that data cannot be combined to target vessels. While this article does demonstrate that the decrease in the probability of casualty is stronger for the South American Region, the Indian Ocean Region and Australia versus North Europe, North America or the Caribbean, it also demonstrates that the data can be combined to target vessels for inspections. Since the time in-between inspections and detention is mostly not significant towards decreasing the probability of casualty, these results reflect the lack of coordination amongst port state control regimes and industry inspections. Due to this lack of coordination and trust, a ship might be inspected in several regimes during a relative short time period where the benefit of an inspection can be easily saturated. Our recommendation on direct policy implication is to promote the harmonization of inspection databases across port state control regimes, preferably with the coordination of the development of the Global Integrated Ship Information System (GISIS) of the International Maritime Organization (IMO), to review the policy of a release of a vessel from detention and to increase cooperation amongst regimes with respect to the follow up of the rectification of deficiencies.     

Longitudinal and depth variation of bacterioplankton productivity and related factors in a temperate estuary

Bacterioplankton productivity (BP) spatial variation was investigated in relation to potential resources, including primary productivity and dissolved organic matter, in the micro-tidal Neuse River–Pamlico Sound estuarine system, North Carolina, USA. Estuarine BP was predicted to correlate with the trophic gradient, decreasing along the salinity gradient in parallel with the decrease in organic matter and primary productivity. This prediction was tested over four years at spatial scales ranging from kilometers to meters along the riverine axis and with depth. The general pattern of BP across the salinity gradient was unimodal and matched the phytoplankton patterns in peak location and variability. Peak locations varied with discharge, especially in 2003 when above average discharge moved peaks downstream. Spatial coherence of BP with other variables was much less at short time scales. The effect of temperature, nutrients, and phytoplankton on BP varied by location, especially fresh versus brackish stations, although only temperature explained more than 20% of the BP variation. Depth variation of BP was as great as longitudinal variation and bottom samples were often higher than surface. BP was strongly correlated with particulate organic carbon at the pycnocline and bottom, highlighting the importance of particulate matter as a resource. Station-averaged BP and phytoplankton data corresponded well with two published meta-analyses, although the offset of the freshwater station suggested longitudinal differences in community composition or resource availability.     

Spatial and seasonal variability of sediment accumulation potential through controlled flooding of the beels located in the polders of the Ganges-Brahmaputra-Meghna delta of Southwest Bangladesh

The Ganges-Brahmaputra-Meghna (GBM) delta plain within Bangladesh is one of the most vulnerable to relative sea level rise (RSLR) in the world especially under current anthropogenically modified (i.e., embanked) conditions. Tidal river management (TRM) as practiced in coastal regions of Bangladesh may provide an opportunity to combat RSLR by raising the land level through controlled sedimentation inside beels (depression within embanked polders) with re-opening of polders. To date, TRM has been applied to tide-dominated coastal regions, but the potential applicability of TRM for the beels within the polders of river-dominated and mixed flow (MF) regimes remains to be assessed. We apply a calibrated 2D numerical hydromorphodynamic model to quantify sediment deposition in a beel flooded through breaching of the polder dike under conditions of river-dominated, tide-dominated and MF regimes for different seasons and applying different regulation schemes for the flow into the beel. Simulation results show considerable seasonality in sediment deposition with largest deposition during the monsoon season. The potential of controlled flooding is highest in the tide-dominated region, where sediment accumulation can be up to 28 times higher than in the river-dominated region. Regulating flow into a beel increases trapping efficiency, but results in slightly lower total deposition than without regulation. We conclude that re-establishing flooding of the beel within the polder without regulating the flow into the beel through breaching of the polder dike is a promising strategy for the mixed and tide-dominated flow regions in the delta as the sediment accumulation can raise the land surface at a higher rate than RSLR and effective SLR (ESLR). In the more upstream river-dominated section of the delta, accumulation rates would be much lower, but the pressure of sea level rise on these areas is lower as well. Owing to the abundant availability of sediment, application of controlled flooding like TRM therefore provides an opportunity to counteract the impact of RSLR and ESLR by means of land raising, particularly along the tidal river reaches in the GBM delta.     

Assessment of three temperature reconstruction methods in the virtual reality of a climate simulation

The performance of statistical climate reconstruction methods in the pre-instrumental period is uncertain, as they are calibrated in a short instrumental period but applied to much longer reconstructions time spans. Here, the virtual reality created by a climate simulation of the past millennium with the model ECHO-G is used as a test bed of three methods to reconstruct the annual Northern Hemisphere temperature. The methods are Composite plus Scaling, the inverse regression method of Mann et al. (Nature 392:779–787, 1998) and a direct principal-components regression method. The testing methodology is based on the construction of pseudo-proxies derived from the climate model output, the application of each of these methods to pseudo-proxy timeseries, and the comparison of their result with the simulated mean temperature. Different structures of the noise have been used to construct pseudo-proxies, ranging from the simulated grid-point precipitation. Also, one sparse and one denser pseudo-proxy network, co-located with two real networks, have been considered. All three methods underestimate the simulated variations of the Northern Hemisphere temperature, but the Composite plus Scaling method clearly displays a better performance and is robust against the different noise models and network size. The most relevant factor determining the skill of the reconstruction appears to be the network size, whereas the different noise models tend to yield similar results.