Tetraploid induction by inhibiting mitosis I in scallopChlamys farreri

Tetraploid induction was carried out by inhibiting mitosis I in fertilized eggs ofChlamys farreri. Mitosis I was blocked with cold shock (5–7°C), Cytochalasin B (0.75 mg/L) and 6-dimethylaminopurine (6-DMAP) (60–75 mg/L) when 60% fertilized eggs released polar body II at 20°C. At 4-cells embryo stage, the ploidy was determined by counting chromosome number. In control groups, most embryos were diploids (72.22%) and aneuploids (24.78%). In Cytochalasin B, cold shock and 6-DMAP treated groups, tetraploids were respectively 10.51%, 4.08%, and 13.34%; aneuploids were 43.10%, 35.93% and 29.16%, and triploids were 7.84%, 8.52% and 18.33%. At D-larva stage, ploidy was determined by flow cytometry (FCM). The ploidy analysis of day 2 larvae showed diploids in control group and also in three treated groups. Juvenile scallops (0.2–0.3cm) which were harvested in two control groups and two CB treated groups were all diploids through checking ploidy individually by FCM. Contribution No. 3648 from the Institute of Oceanology, Chinese Academy of Sciences. Contribution No. 238 from the Experimental Marine Biology Laboratory, Chinese Academy of Sciences. Project funded by grant (No. 819-01-07) from the Chinese Science and Technology Ministry and also by “Hundred Talents Plan” grant from the Chinese Academy of Sciences.     

Pollution trend in the tumen river and its influence on regional development

１ＩＮＴＲＯＤＵＣＴＩＯＮＳｅｖｅｒｅｗａｔｅｒｐｏｌｕｔｉｏｎａｎｄｗａｔｅｒｒｅｓｏｕｒｃｅｓｓｈｏｒｔａｇｅｓｈａｖｅｂｅｃｏｍｅｔｗｏｋｅｙｏｂｓｔｒｕｃｔｉｏｎｓｔｏｒｅａｌｉｚｅｓｕｓｔａｉｎａｂｌｅｕｔｉｌｉｚａｔｉｏｎｏｆｗａｔｅ...     

倾斜平面二维渗流与隧道涌水量计算基本理论

倾斜平面内的渗流问题在倾斜岩层与裂隙岩层地区,特别是在这类岩层地区开凿隧道、巷道等地下工程时是常见的。但是,这类问题的系统基本理论则很少见到,特别是将这类问题与隧道涌水量计算相联系的理论则更罕见。就此问题,给出了这类渗流问题的坐标系的选取与各种基本方程、定解条件、数学模型、隧道在倾斜剖面中的形体变态理论和两类隧道单宽涌水量的转化等较系统、较完整的一套基本理论。这些理论对实际工程和理论发展都有重要意义。     

马超营断裂带区域地球化学特征

该文探讨了马超营断裂带内各地层的成矿元素丰度特征,阐述了该带内成矿元素的分布特征及分布规律,总结了Ａｕ及有关元素Ａｇ、Ｐｂ、Ｚｎ、Ｍｏ、Ｃｏ、Ｎｉ、Ｍｎ、Ｂａ、Ｓｒ、Ａｓ、Ｓｂ、Ｂｉ等异常的特征及其分布规律。研究成果表明：马超营断裂带的韧性—韧脆性剪切带是本区Ａｕ及与其相关的Ａｇ、Ｐｂ、Ｚｎ等成矿元素异常规模、形态和空间分布的控制因素。     

加强科研前期管理提高科研立项申报质量

针对成都气象学院近年来科研项目申报中存在的问题,提出了选题必须遵循的基本原则,填写申报书和加强申报工作管理的各环节。这对提高项目科学水平和获准率具有一定作用。     

Comparison of different land-surface perturbation methods in short-range ensemble forecasts

为了比较不同陆面扰动方法对短时集合预报的影响,本研究设计了陆面模式扰动实验(LSMPE),初始土壤湿度扰动实验(ISMPE),陆面-大气耦合系数扰动实验(LCCPE)以及大气扰动对照实验(GEFSPE).结果表明,在三组陆面扰动实验中,LSMPE能代表最大的不确定性且误差最小;ISMPE的离散度要比LCCPE稍大,但是...     

Description of deformation process in inherently anisotropic granular materials

The main focus in this work is on modeling of mechanical response of granular materials that display inherent anisotropy. Both the experimental and numerical investigations are described. First, the results of direct shear as well as drained/undrained triaxial tests that involve crushed limestone with elongated angular‐shaped particles are reviewed. Afterward, a mathematical framework is presented for modeling of elastic/ inelastic deformation that incorporates the multi‐laminate approach. The deformation is monitored on a set of randomly oriented planes, and the formulation incorporates the thickness of the shear band that is associated with sliding/separation process. A systematic procedure for identification of material functions/ parameters is outlined that is based on the results of direct shear tests, and the framework is later applied to simulate the behavior under triaxial conditions. The results of numerical simulations are compared with the experimental data. Copyright © 2011 John Wiley & Sons, Ltd.     

泡沫塑料吸附测金中无臭灰化技术的改进

根据泡沫塑料(简称泡塑)吸附测金的无臭灰化技术的机理,作者采用了3ml圆底瓷坩埚灰化,解决了大批量佯品的灰化问题和电极装样困难的问题,并节省了灰化用的滤纸。     

Characteristics of the temporal-spatial variation in atmospheric ozonosphere over the northern hemisphere during the period of 1963-1985

The total ozone data of 113 ozone observational stations in the Northern Hemisphere (NH) spanning a period of 1963-1985 have been analysed in this paper. Some interesting results have been obtained, such as the temporal and spatial distribution, long-term trends, the harmonic analysis results, the relationship between the total ozone and solar activity, etc. Furthermore, by using the denned index G, the long-term changes of ozone meridional distribution patterns for the region of Europe-Asia (EA) have been approached particularly.