Geotechnical and Geological Engineering - A set of splitting cracks often occur in the high side wall of the deep underground hydropower station due to the excavation-induced unloading during the... 相似文献
Geotechnical and Geological Engineering - The second phase of the Chongqing Rail Transit Line project has poor rock and soil stability, slow construction progress, complex and variable geological... 相似文献
Acta Geochimica - Isotopic signature is a powerful tool to discriminate methane (CH4) source types and constrain regional and global scale CH4 budgets. Peatlands on the Qinghai-Tibetan Plateau are... 相似文献
Computational Geosciences - In order to quantify the performance ranking of oil wells in high water cut reservoir, a quantitative evaluation method of oil wells is established from two perspectives... 相似文献
Landslides - In this paper, the slope rock mass is numerically characterized by considering hydraulic and mechanical properties using two distinct element models. The flow rate and permeability are... 相似文献
In certain field conditions such as offshore projects under wave loads or embankments under traffic loads, both the vertical and horizontal stresses are variable. However, previous investigations rarely considered the variation in horizontal stress. To better understand the characteristics of natural saturated soft clay, a series of monotonic and cyclic triaxial tests with a K0-consolidation state were carried out under a variable confining pressure (VCP) stress path. The development of axial strain, pore water pressure and effective stress path is analysed. The results show that with the increase in η (the ratio of the variation in the mean effective principal stress to that of the deviatoric stress), the undrained shear strength (qf) decreases continuously. The pore water pressure generation is slightly improved under a stress path with increasing confining pressure. Based on the test results, a unified formula was established to predict the pore water pressure under VCP stress paths. The unique p–q–e relationship of normally consolidated clay in monotonic VCP triaxial tests was also demonstrated. Under VCP stress paths, the amplitude of the pore pressure increases, and the effective stress path tilts more sharply to the right. Moreover, a unified formula was established that can provide a good reference for predicting effective stress paths under cyclic VCP triaxial tests.
Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 （PP1） is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study, a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase l（PPlcb）, was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus, designated SmPPlcb, by the rapid amplification of cDNA ends （RACE） technique. The cDNA sequence of SmPPlcb we obtained contains a 984 bp open reading frame （ORF）, flanked by a complete 39 bp 5＇ untranslated region and 462 bp 3＇ untranslated region. The ORF encodes a putative 327 amino acid protein, and the N-terminal section of this protein is highly acidic, Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp, a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B （PP2B）. And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that, SmPPlcb is extremely conserved in both amino acid and nucleotide acid levels compared with the PPlcb of other vertebrates and invertebrates, and its Kozak motif contained in the 5＇UTR around ATG start codon is GXXAXXGXXATGG, which is different from mammalian in two positions A6 and G3, indicating the possibility of different initiation of translation in turbot, and also the 3＇UTR of SmPPlcb is highly diverse in the sequence similarity and length compared with other animals, especially zebraf＇lsh. The cloning and sequencing of SmPPlcb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot. 相似文献
A model integrating geo-information and self-organizing map (SOM) for exploring the database of soil environmental surveys was established. The dataset of 5 heavy metals (As, Cd, Cr, Hg, and Pb) was built by the regular grid sampling in Hechi, Guangxi Zhuang Autonomous Region in southern China. Auxiliary datasets were collected throughout the study area to help interpret the potential causes of pollution. The main findings are as follows: (1) Soil samples of 5 elements exhibited strong variation and high skewness. High pollution risk existed in the case study area, especially Hg and Cd. (2) As and Pb had a similar topo-logical distribution pattern, meaning they behaved similarly in the soil environment. Cr had behaviours in soil different from those of the other 4 elements. (3) From the U-matrix of SOM networks, 3 levels of SEQ were identified, and 11 high risk areas of soil heavy metal-contaminated were found throughout the study area, which were basically near rivers, factories, and ore zones. (4) The variations of contamination index (CI) followed the trend of construction land (1.353) > forestland (1.267) > cropland (1.175) > grassland (1.056), which suggest that decision makers should focus more on the problem of soil pollution surrounding industrial and mining enterprises and farmland.
