Preservation conditions and the use of sediment pigments as a tool for recent ecological reconstruction in four Northern European estuaries

Down-core sediment pigment concentrations from four Northern European estuaries were measured using high-performance liquid chromatography (HPLC) to investigate phytoplankton community structure and preservation conditions over the last ca. 100 years where all sites have experienced different levels of eutrophication. Phytoplankton pigments have been shown to be useful biomarkers for phytoplankton community structure and abundance due to their taxonomic specificity. The pigment concentrations and sediment pigment inventory showed large variation between the four sites. Concentrations ranged from more than 6000 nmol/g OC to less than 100 nmol/g OC and the inventory integrated over the top 10 cm from more than 300 nmol/cm² to less than 30 nmol/cm² for total identified pigments. Good pigment preservation in Mariager Fjord (Denmark) reflected the almost permanently anoxic conditions. Pigments in Laajalahti (Finland) showed peak concentrations around the time of highest nitrogen loading events known from historical and modelled records over the past 100 years. In contrast, poor down-core preservation of pigments (especially carotenoids) was observed in the Ems-Dollard (The Netherlands) and Himmerfjärden (Sweden) estuaries. The Ems-Dollard site is an intertidal mudflat that experiences daily exposure to light and air, which enhances pigment degradation. In Himmerfjärden, resuspension is an important process affecting both the sedimentation rate and degradation properties. The different preservation conditions at the four sites were supported by the differences in two degradation indicators; the ratio of pheopigment-a to chlorophyll-a and total carotenoids to total pigments. Class-specific carotenoid pigments represented the dominant algal groups reported from each site, however, no distinct down-core changes in the pigment composition were observed at any of the four sites. This indicated that changes in plankton community structure on the group level have been limited over this time period or masked by low preservation of pigments.     

Modeling of oil spreading in a problem of radar multiangle diagnostics of Sea surface pollutions

The possibilities of a multiangle method of radar diagnostics to determine thickness of an oil film on a sea surface by comparing the radar data with the quantitative modeling results obtained using the model of oil spreading dynamics are analyzed. The experimental results of the remote sensing of the Caspian Sea water area near the Neftyanye Kamni oil field by the Envisat-1 synthetic aperture radar (SAR) and the new Floating Objects Tracking System (FOTS) model of oil spreading are used for the analysis. The model allows to calculate the dynamics and change in the mass and size of an oil slick basing only on the available data of satellite measurements and atmospheric reanalysis.The model takes into account the main processes that influence the slick formation (gravity spreading, advective transport, dispersion, emulsification, turbulent mixing, and evaporation). This model is used to calculate the thickness evolution and dynamics of the displacement of oil slicks in the period between two consecutive radar images of this region (0.5–4 days) and to estimate the volumes of oil spilled in the field. The good consistence of the height of the oil film calculated using radar measurements and the modeling results confirms the method’s reliability.     

Experimental studies of thermal radiation intensity dependence on near-water wind speed for rough sea surface

The results of in situ measurements of the characteristics of intrinsic microwave radiation from the sea surface are presented. The studies were carried out on the oceanographic platform of the Experimental Department of Marine Hydrophysical Institute during the summer–fall periods for 3 years, which made it possible to accumulate a significant amount of information on the change in the radiation intensity of under different meteorological conditions. Attention is primarily focused on the construction of wind-radiation dependences and their steepness analysis based on the measurement data obtained by a radiometer-polarimeter with a central frequency of received radiation equal to 37.7 GHz. The results are compared with the previous experimental studies and the model estimates of this effect.     

Viruses,bacteria, and heterotrophic nanoflagellates in Laptev Sea plankton

The paper considers the concentrations and functional characteristics of viruses, bacteria, and heterotrophic nanoflagellates determined for the first time in the Laptev Sea in August-September, 2014. The abundance of bacteria, viruses, and heterotrophic nanoflagellates varied from 110.1 × 10³ to 828.4 × 10³ cells/mL, from 384.2 × 10³ to 2932.8 × 10³ particles/mL, and from 108 to 651 cells/mL, respectively. The daily bacterioplankton production varied from 4.2 × 10³ to 381.7 × 10³ cells/mL, with an average of 117.6 × 10³ cells/mL. Electron transmission microscopy has for the first time shown that the frequency of visibly infected bacterial cells varied from 0.2 to 2.0% (0.8% on average) of N_B. The average virus-induced mortality of bacteria was 6.3% of bacterioplankton production, with variations ranging from 1.4 to 16.9%. Grazing on bacteria by heterotrophic nanoflagellates contributed more to bacteria mortality than virus-induced bacterial lysis. By grazing on bacteria, heterotrophic nanoflagellates consumed large quantities of viruses located on the surface and inside bacterial cells.     

Exploring the spatial distribution patterns of South African Cape hakes using generalised additive models

We developed delta generalised additive models (GAMs) to predict the spatial distribution of different size classes of South African hakes, Merluccius capensis and M. paradoxus, using demersal trawl survey data and geographical (latitude and longitude) and environmental features (depth, temperature, bottom dissolved oxygen and sediment type). Our approach consists of fitting, for each hake size class, two independent models, a binomial GAM and a quasi-Poisson GAM, whose predictions are then combined using the delta method. Delta GAMs were validated using an iterative cross-validation procedure, and their predictions were then employed to produce distribution maps for the southern Benguela. Delta GAM predictions confirmed existing knowledge about the spatial distribution patterns of South African hakes, and brought new insights into the factors influencing the presence/absence and abundance of these species. Our GAM approach can be used to produce distribution maps for spatially explicit ecosystem models of the southern Benguela in a rigorous and objective way. Ecosystem models are critical features of the ecosystem approach to fisheries, and distribution maps constructed using our GAM approach will enable a reliable allocation of species biomasses in spatially explicit ecosystem models, which will increase trust in the spatial overlaps and, therefore, the trophic interactions predicted by these models.     

Population genetic diversity and structure of a dominant tropical seagrass,Cymodocea rotundata,in the Western Pacific region

Cymodocea rotundata is an ecologically important tropical pioneer seagrass species distributed in the Indo‐Pacific region. The population genetic diversity and structure of this species were analysed at 46 sites spanning the Philippines, Ryukyu Islands (northern limit) and Hainan Island, by using microsatellite simple sequence repeat (SSR) markers. Analyses revealed the persistence of C. rotundata likely relies on local population dynamics and fitness influenced by environmental gradients, with sexual reproduction prevalent in the Philippines while the Ryukyu and Hainan populations were predominantly established by clonal spread. Analysis of molecular variance showed significant genetic differentiation (P < 0.001) among three geographic regions: the Philippines, Ryukyu and Hainan. Furthermore, the mean fixation index value was very high (F_ST = 0.36), indicating poor dispersal potential or limited gene flow. Allelic richness and heterozygosity of C. rotundata was comparable in the Philippines and Ryukyu Islands populations. More private alleles were found in the Philippines and excess heterozygotes in the Ryukyu Islands. STRUCTURE analysis revealed that the Ryukyu and Hainan populations were mosaics of admixed alleles of individuals from the Philippines. An assignment test suggested that recruitment occurs from the Northeast Philippines to Ryukyu Islands. These results suggest that the Philippine populations are tropical seagrass hotspots and perhaps the origin of the populations in the Ryukyu and Hainan Islands. This scenario is most likely driven by the Kuroshio Current and island integration events during plate tectonic activities. The Philippine Archipelago is considered of high importance for conservation objectives and management plan of seagrasses. Likewise, the Ryukyu Islands are also important in terms of conserving regional diversity because locally adapted genotypes have important evolutionary potential in the face of environmental change.     

不同刚度植物杆群对规则波传播及紊动特性影响研究

以不同刚度硅胶圆杆群为概化植物模型,测定其抗弯弹性模量,通过波浪水槽实验,研究规则波在不同刚度植物杆群内的流速分布、紊动特征及不同刚度杆群的消浪效果。实验结果表明,当波浪通过柔性杆群时,受其摆动的影响,流速周期变化从单峰型逐渐转变成双峰型,杆群刚度越小形成的二次波峰越明显;不同刚度杆群内水体紊动强度变化显示,杆群刚度越大,造成杆群内水体的紊动强度越大;随着杆群抗弯弹性模量的增大,其消浪系数也增大,消浪系数的增长与材料的抗弯弹性模量值非线性关系,而是在某一弹性模量范围内,对消浪系数的影响较为敏感。     

Fine‐scale diet of the Australian sea lion (Neophoca cinerea) using DNA‐based analysis of faeces

We applied DNA‐based faecal analysis to determine the diet of female Australian sea lions (n = 12) from two breeding colonies in South Australia. DNA dietary components of fish and cephalopods were amplified using the polymerase chain reaction and mitochondrial DNA primers targeting the short (~100 base pair) section of the 16S gene region. Prey diversity was determined by sequencing ~50 amplicons generated from clone libraries developed for each individual. Faecal DNA was also combined and cloned from multiple individuals at each colony and fish diversity determined. Diets varied between individuals and sites. Overall, DNA analysis identified a broad diversity of prey comprising 23 fish and five cephalopod taxa, including many species not previously described as prey of the Australian sea lion. Labridae (wrasse), Monacanthidae (leatherjackets) and Mullidae (goat fish) were important fish prey taxa. Commonly identified cephalopods were Octopodidae (octopus), Loliginidae (calamary squid) and Sepiidae (cuttlefish). Comparisons of fish prey diversity determined by pooling faecal DNA from several samples provided a reasonable but incomplete resemblance (55–71%) to the total fish diversity identified across individual diets at each site. Interpretation of diet based on the recovery of prey hard‐parts identified one cephalopod beak (Octopus sp.) and one fish otolith (Parapriacanthus elongatus). The present study highlights the value of DNA‐based analyses and their capabilities to enhance information of trophic interactions.