Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels.     

The optimum control of thruster system for dynamically positioned vessels

An offshore vessel with a dynamic positioning system (DP system) needs fast response to produce thrust to counteract the environmental forces acting on it for the purpose of maintaining its position and heading as close as possible to the working position. Therefore, quick and effective modulation of the thrust is the problem to determine the thrust and the rotation angle of the thruster devices of the ship. This paper presents an effective optimum control for a thruster system, using the sequential quadratic method to achieve economical and effective modulation of the thrust and the direction of the thruster. An optimum control study of a 2280 tons DP coring vessel with five rotary azimuth thruster marine positioning is studied in detail, which can quickly and exactly estimate the thrusts and angles of direction of all the thrusters. The results can provide a valuable thruster system for a dynamically positioned vessel.     

Observed foundation behaviour of concrete gravity platforms installed in the North Sea 1973–1978

Available measurements of foundation behaviour of the 13 concrete gravity platforms installed in the North Sea in the years 1973–1978 are presented. The measurements cover both the installation phase and the early part of the operation period. For the installation phase, measurements of dowel and skirt penetration resistance, base contact stresses, tilt, piping and erosion, are presented. For the operation period, the presentation includes measurements of settlements, pore water pressures, base contact stresses, permanent lateral displacements, cyclic displacements, dynamic behaviour and erosion. The measurements are used to back calculate soil properties and to evaluate the adequacy of the design procedures which have been used. The observations give valuable experience with respect to the foundation performance of offshore gravity platforms.     

Submerged cylinder wave energy device: theory and experiment

Linearized water wave theory is used to show that a submerged long circular cylinder suitably constrained by springs and dampers to make small harmonic oscillations, can be extremely efficient in absorbing the energy in an incident regular wave whose crests are parallel to the axis of the cylinder. Experimental results are described which confirm the theory for small amplitude waves and which suggest that the device can still be fairly efficient in waves of moderate amplitude.     

Solution of an unstable axisymmetric Cauchy–Poisson problem of dispersive water waves for a spectrum with compact support

 It has been known that the axisymmetric Cauchy–Poisson problem for dispersive water waves is well posed in the sense of stability. Thereby time evolution solutions of wave propagation depend continuously on initial conditions. However, in this paper, it is demonstrated that the axisymmetric Cauchy–Poisson problem is ill posed in the sense of stability for a certain class of initial conditions, so that the propagating solutions do not depend continuously on the initial conditions. In order to overcome the difficulty of the discontinuity, Landweber–Fridman's regularization, famous and well known in applied mathematics, are introduced and investigated to learn whether it is applicable to the present axisymmetric wave propagation problem. From the numerical experiments, it is shown that stable and accurate solutions are realized by the regularization, so that it can be applicable to the determination of the ill-posed Cauchy–Poisson problem.     

Self-noise measurements using a moored sonobuoy with a suspended hydrophone

Moored sonobuoys are used for refraction seismic measurements at sea, although the range of operation is limited by the self noise caused by the hydrophone suspension.In this paper the main causes of self noise are discussed and a useful deployment scheme of a moored sonobuoy with suspended hydrophone is described.Some design criteria, in particular an equation for calculation of the optimal length of the expandable rubber band-which is situated between the surface float and the damping-body-are discussed.Finally, it is shown from noise measurements during the F. S. Valdivia cruise in 1975, that self noise level in the band 2 Hz to 20 Hz is 180 mPa to 280 mPa at a fully developed windsea with a characteristic wave height of more than 4 meters and a waterstream velocity about 0.5 m s¹.During these measurements the hydrophone was attached to the cable without rubber band. It is concluded that at a normal seastate the self noise level can be reduced to the order of ocean floor noise.     

In vitro alternatives to whole animal testing. Comparative cytotoxicity studies of divalent metals in established cell lines derived from tropical and temperate water fish species in a neutral red assay

A simple vital dye (neutral red) uptake method for investigation of the acute toxicity of seven divalent metal cations to cell lines derived from the turbot (TF) and bluegill (BF-2) was evaluated. The method was extremely reproducible between replicate experiments on a week to week basis as well as being reproducible between different laboratories. Whilst there were some species-dependent differences in the relative toxicities of the metals there were good correlations between toxicity and the chemical softness (Σp) of the metal ions and also between in vivo and in vitro toxicities. These studies demonstrate that this simple and relatively inexpensive technique carried out in 96-well microplates may be extremely useful for evaluation of structure-toxicity relationships between related groups of compounds.