The US freedom of navigation program preserving the law of the sea

The 1982 United Nations Convention on the Law of the Sea prescribed extensive rules of behavior for the international community in the oceanic regime. Some of the most important provisions of the Convention are those regarding the freedom of navigation and overflight in various maritime zones, i.e. territorial seas, international straits, archipelagos, exclusive economic zones and the high seas.While most coastal nations are complying with the navigation and overflight provisos of the Convention, some are not. Where excessive maritime claims exist, the US has evolved an ambitious Freedom of Navigation (FON) Program which challenges these excessive claims. This Program was initiated under the Carter Administration in 1979 and has continued, unabated, ever since.As the international community prepares for the day when the 1982 Convention becomes universally-recognized international law, there are likely to be periodic excessive maritime claims. In cases where the Convention's dispute settlement provisos fail to resolve excessive claims, the community of nations may find it useful to use the US FON Program as an example for the enforcement of maritime rules.     

Alternatives to whole animal testing: Use of cDNA probes for studies of phase I and II enzyme induction in isolated plaice hepatocytes

Studies of xenobiotic metabolism and the regulation of enzyme systems for their metabolism (Phase I and II enzyme systems) require large numbers of animals, intensive use of experimental aquarium systems and in some instances can pose major problems when the compounds are scarce, expensive or too toxic to be disposed of easily. Previous studies have demonstrated the usefulness of isolated primary hepatocytes of fish for metabolic and enzyme induction studies. Functionally and structurally competent hepatocytes were isolated from juvenile plaice and after overnight acclimation to culture conditions, were exposed to a variety of PAHs for 24h. The levels of CYP1A1, phenol UDPGT and GST-A mRNAs were then estimated by slot blotting and hybridisation to their cDNA probes. The method enabled rapid and easy determination of the structure/activity relationships of these compounds as inducers of these key Phase I and II enzymes, and is potentially useful for screening large numbers of compounds both as an invitro toxicity test and for mechanistic studies.     

Comparison of xenobiotic metabolizing enzymes of Tilapia with those of other fish species and interspecies relationships between gene families

Baseline data for hepatic xenobiotic metabolizing biomarker enzyme activities were obtained for artificially reared tilapia Oreochromis niloticus, and were compared with those of the plaice (Pleuronectes platessa) and rainbow trout (Onchorynchus mykiss). Basal activities exhibited species variations with notably higher CYP1A and phenol UGT activities and lower GST activity in plaice than the freshwater species. Interspecies relationships between gene families determined by immunoblotting and substrate-activity profiles demonstrated the presence of homologous CYP1A and CYP3A enzymes in all three species, alpha class GSTs in plaice and trout, mu and pi class GSTs in trout and theta class GSTs in plaice and tilapia. CYP1A of tilapia was induced by 3-MC or PBO treatment, whilst CYP3A was induced by PCN treatment.     

Current-generated noise recorded on ocean bottom seismometers

High-amplitude, anrrow band noise that correlates with periods of high ocean bottom currents and the tidal cycle is occasionally observed on ocean bottom seismometers (OBS). The geophones on OBSs of different configurations are not equally sensitive to this noise and hydrophones are almost unaffected. With a suitable design, it should be possible to eliminate this noise problem.Hawaii Institute of Geophysics Contribution No. 1173     

Numerical Simulation of the Gulf Stream and the Deep Circulation

The Gulf Stream system has been numerically simulated with relatively high resolution and realistic forcing. The surface fluxes of the simulation were obtained from archives of calculations from the Eta-29 km model which is an National Center for Environment Prediction (NCEP) operational atmospheric prediction model; synoptic fields are available every 3 hour. A comparison between experiments with and without surface fluxes shows that the effect of the surface wind stress and heat fluxes on the Gulf Stream path and separation is closely related to the intensification of deep circulations in the northern region. Additionally, the separation of the Gulf Stream and the downslope movement of the Deep Western Boundary Current (DWBC) are reproduced in the model results. The model DWBC crosses under the Gulf Stream southeast of Cape Hatteras and then feeds the deep cyclonic recirculation east of the Bahamas. The model successfully reproduces the cross-sectional vertical structures of the Gulf Stream, such as the asymmetry of the velocity profile, and this structure is sustained along the downstream axis. The distribution of Root Mean Square (RMS) elevation anomaly of the model shows that the eddy activity of the Gulf Stream is realistically reproduced by the model physics. The entrainment of the upper layer slope current into the Gulf Stream occurs near cross-over; the converging cross-stream flow is nearly barotropic. This revised version was published online in August 2006 with corrections to the Cover Date.