Cloning of the major 3-MC inducible phase I and phase II detoxification enzymes of plaice liver

cDNA's coding for cytochrome P4501A1 (CYP1A1), phenol UDP-glucuronosyltransferase (UDPGT) and glutathione S-transferase (GST-A) were cloned and sequenced from an expression library prepared from the liver of a 3-methylcholanthrene (3-MC) induced plaice. Plaice CYP1A1 and Phenol UDPGT display a high degree of structural conservation with homologous mammalian isoforms and their mRNAs were shown to be highly induced in liver after 3-MC treatment of fish. Expression of plaice GST-A, which displays closer homology to GSTs from plants and invertebrates than those of mammals, is repressed after 3-MC treatment.     

Voltammetric estimation of iron(III) thermodynamic stability constants for catecholate siderophores isolated from marine bacteria and cyanobacteria

Thermodynamic stability constants have been estimated for the complexation of iron(III) with catecholate-type siderophores isolated from the marine bacterium Alteromonas luteoviolacea and from the marine cyanobacterium Synechococcus sp. PCC 7002. Stability constants were determined utilizing the “chelate scale” of Taylor et al. (1994). The scale is based upon a linear relationship between the reduction potentials and the pH-independent thermodynamic stability constants for known iron(III) complexes. Log K values for the alterobactin B ferric iron complex are 43.6 ± 1.5 at pH 8.2 and 37.6 ± 1.2 at pH 6, consistent with a shift from bis-catecholate to monosalicylate/monocatecholate iron coordination with decreasing pH. Synechococcus isolates PCC 7002 Nos. 1 and 3 formed iron(III) complexes with stability constants of approximately 38.1 ± 1.2 and 42.3 ± 1.5, respectively. The binding strengths of the iron(III) complexes examined in this study are quite high, suggesting that catecholate siderophores may play a role in the solubilization and biological uptake of iron in the marine environment.     

Nitrate reductase activity and the speciation of selenium at the mouth of Chesapeake bay

Nitrate reductase activity and the concentrations of selenite and selenate were monitored for six months at the mouth of Chesapeake Bay (USA). A positive correlation was found between nitrate reductase activity and the concentration of selenite, suggesting that selenite may be formed in coastal waters and nitrate reductase may be involved in the process.     

The depletion of carbon by extra mixing in metal-poor giants

There is an apparent dichotomy between the metal-poor  ([Fe/H]≤−2)  yet carbon-normal giants and their carbon-rich counterparts. The former undergo significant depletion of carbon on the red giant branch after they have undergone first dredge-up, whereas the latter do not appear to experience significant depletion. We investigate this in the context that the extra mixing occurs via the thermohaline instability that arises due to the burning of  ³He  . We present the evolution of [C/Fe], [N/Fe] and  ¹²C/¹³C  for three models: a carbon-normal metal-poor star, and two stars that have accreted material from a  1.5 M_⊙  AGB companion, one having received  0.01 M_⊙  of material and the other having received  0.1 M_⊙  . We find the behaviour of the carbon-normal metal-poor stars is well reproduced by this mechanism. In addition, our models also show that the efficiency of carbon-depletion is significantly reduced in carbon-rich stars. This extra-mixing mechanism is able to reproduce the observed properties of both carbon-normal and carbon-rich stars.     

Evidence for a binary companion to the central compact object 1E 1207.4-5209

Unique among neutron stars, 1E 1207.4-5209 is an X-ray pulsar with a spin period of 424 ms that contains at least two strong absorption features in its energy spectrum. This neutron star is positionally coincident with the supernova remnant PKS 1209-51/52 and has been identified as a member of the growing class of radio-quiet compact central objects in supernova remnants. From previous observations with Chandra and XMM-Newton, it has been found that the 1E 1207.4-5209 is not spinning down monotonically as is common for young, isolated pulsars. The spin frequency history requires either strong, frequent glitches, the presence of a fall-back disk, or a binary companion. Here, we report on a sequence of seven XMM-Newton observations of 1E 1207.4-5209 performed during a 40 day window between 2005 June 22 and July 31. Due to unanticipated variance in the phase measurements during the observation period that was beyond the statistical uncertainties, we could not identify a unique phase-coherent timing solution. The three most probable timing solutions give frequency time derivatives of +0.9, ?2.6, and +1.6×10^?12 Hz s^?1 (listed in descending order of significance). We conclude that the local frequency derivative during our XMM-Newton observing campaign differs from the long-term spin-down rate by more than an order of magnitude. This measurement effectively rules out glitch models for 1E 1207.4-5209. If the long-term spin frequency variations are caused by timing noise, the strength of the timing noise in 1E 1207.4-5209 is much stronger than in other pulsars with similar period derivatives. Therefore, it is highly unlikely that the spin variations are caused by the same physical process that causes timing noise in other isolated pulsars. The most plausible scenario for the observed spin irregularities is the presence of a binary companion to 1E 1207.4-5209. We identified a family of orbital solutions that are consistent with our phase-connected timing solution, archival frequency measurements, and constraints on the companions mass imposed by deep IR and optical observations.     

The early-type galaxies NGC 1407 and NGC 1400 – I. Spatially resolved radial kinematics and surface photometry

This is the first paper of a series focused on investigating the star formation and evolutionary history of the two early-type galaxies NGC 1407 and NGC 1400. They are the two brightest galaxies of the NGC 1407 (or Eridanus-A) group, one of the 60 groups studied as part of the Group Evolution Multi-wavelength Study.
Here, we present new high signal-to-noise ratio long-slit spectroscopic data obtained at the ESO 3.6-m telescope and high-resolution multiband imaging data from the Hubble Space Telescope /Advanced Camera for Surveys and wide-field imaging from Subaru Suprime-Cam. We spatially resolved integrated spectra out to ∼0.6 (NGC 1407) and ∼1.3 (NGC 1400) effective radii. The radial profiles of the kinematic parameters v _rot, σ, h ₃ and h ₄ are measured. The surface brightness profiles are fitted to different galaxy light models and the colour distributions analysed. The multiband images are modelled to derive isophotal shape parameters and residual galaxy images. The parameters from the surface brightness profile fitting are used to estimate the mass of the possible central supermassive black hole in NGC 1407. The galaxies are found to be rotationally supported and to have a flat core in the surface brightness profiles. Elliptical isophotes are observed at all radii and no fine structures are detected in the residual galaxy images. From our results, we can also discard a possible interaction between NGC 1400, NGC 1407 and the group intergalactic medium. We estimate a mass of  ∼1.03 × 10⁹ M_⊙  for the supermassive black hole in NGC 1407 galaxy.     

Large-scale galactic turbulence: can self-gravity drive the observed H i velocity dispersions?

Observations of turbulent velocity dispersions in the H  i component of galactic discs show a characteristic floor in galaxies with low star formation rates and within individual galaxies the dispersion profiles decline with radius. We carry out several high-resolution adaptive mesh simulations of gaseous discs embedded within dark matter haloes to explore the roles of cooling, star formation, feedback, shearing motions and baryon fraction in driving turbulent motions. In all simulations the disc slowly cools until gravitational and thermal instabilities give rise to a multiphase medium in which a large population of dense self-gravitating cold clouds are embedded within a warm gaseous phase that forms through shock heating. The diffuse gas is highly turbulent and is an outcome of large-scale driving of global non-axisymmetric modes as well as cloud–cloud tidal interactions and merging. At low star formation rates these processes alone can explain the observed H  i velocity dispersion profiles and the characteristic value of  ∼10 km s⁻¹  observed within a wide range of disc galaxies. Supernovae feedback creates a significant hot gaseous phase and is an important driver of turbulence in galaxies with a star formation rate per unit area  ≳10⁻³ M_⊙ yr⁻¹ kpc⁻²  .     

The use of invariant manifolds for transfers between unstable periodic orbits of different energies

Techniques from dynamical systems theory have been applied to the construction of transfers between unstable periodic orbits that have different energies. Invariant manifolds, trajectories that asymptotically depart or approach unstable periodic orbits, are used to connect the initial and final orbits. The transfer asymptotically departs the initial orbit on a trajectory contained within the initial orbit’s unstable manifold and later asymptotically approaches the final orbit on a trajectory contained within the stable manifold of the final orbit. The manifold trajectories are connected by the execution of impulsive maneuvers. Two-body parameters dictate the selection of the individual manifold trajectories used to construct efficient transfers. A bounding sphere centered on the secondary, with a radius less than the sphere of influence of the secondary, is used to study the manifold trajectories. A two-body parameter, κ, is computed within the bounding sphere, where the gravitational effects of the secondary dominate. The parameter κ is defined as the sum of two quantities: the difference in the normalized angular momentum vectors and eccentricity vectors between a point on the unstable manifold and a point on the stable manifold. It is numerically demonstrated that as the κ parameter decreases, the total cost to complete the transfer decreases. Preliminary results indicate that this method of constructing transfers produces a significant cost savings over methods that do not employ the use of invariant manifolds.