胶州湾沉积物中石油降解菌的降解能力及多样性研究

2013年青岛输油管道爆炸,大量石油污染了附近海岸。课题组采集了污染的沉积物样品,以原油为唯一碳源和能源,富集了四个石油降解菌群。生物多样性和群落分析表明,Luteibacter、Parvibaculum 和属于食烷菌科的一个属是降解菌群的主要优势菌,都属于变形菌门。从石油降解菌群中分离筛选,获得了9株具有不同16S rRNA基因序列的降解菌,分别属于8个属。重量法测定降解菌的石油降解率,其中5株的石油降解率大于30%。GC-MS分析结果表明,石油降解菌多倾向于降解烷烃,对多环芳烃的降解能力较差,其中5株细菌的烷烃降解率较大,仅1株菌D2对多环芳烃的降解率较大,其降解率在34.9%到77.5%。通过对高通量数据的分析,表明食烷菌属是菌群A和菌群E的主要降解菌群,其中筛选获得的菌株E4可能是菌群E的一株优势降解菌。本研究所筛选菌株证明了其石油降解潜力,为油污染海滩生物修复提供了菌株资源。     

Long-term monitoring of the individual self-feeding behavior of rainbow trout Oncorhynchus mykiss

Self-feeding device is extensively used in aquaculture farms, but for salmonids the individual feeding behavior has seldom been continuously observed. In this article, the individual self-feeding behavior of 10 rainbow trout was continuously monitored with a PIT tag record for 50 days with three replicates. The fish fell into three categories according to their feeding behavior, i.e. high triggering fish (trigger behavior more than 25% of the group, HT), low triggering fish (1%–25%, LT) and zero triggering fish (less than 1%). The results showed that in a group of 10 individual 1–2 HT fish accounted for most of the self-feeding behavior (78.19%–89.14%), which was far more than they could consume. The trigger frequency of the fish was significantly correlated with the initial body weight (P <0.01), however, no significant difference in growth rate among the HT, LT, and ZT fish was observed (P >0.05). Cosinor analysis showed that the two HT fish in the same group had similar acrophase. Though some of the HT fish could be active for 50 d, there were also HT fish decreased triggering behavior around 40 d and the high trigger status was then replaced by other fish, which was first discovered in salimonds. Interestingly, the growth of the group was not affected by the alternation triggering fish. These results provide evidence that in the self-feeding system the HT fish didn’t gain much advantage by their frequent self-feeding behavior, and high trigger status of the HT fish is not only an individual character but also driven by the demand of the group. In the self-feeding system, the critical individual should be closely monitored.

     

广州城市流浪乞讨者的空间管治与日常生活实践

以广州流浪乞讨者为研究对象,通过深度访谈等质性研究方法,解读针对流浪乞讨者的空间管治政策与手段,分析流浪乞讨者的日常生活实践与生境协商。研究发现：在地方城市政策对流动性与公共秩序限制的双重压力下,在流浪乞讨者的日常生活实践中,不仅有针对管治行动者而刻意为之的特殊营建,亦有基于程式化生活流程对自我身份认同的重塑与真情实感的展演。地方政府基于空间目标的救助与真实存在的日常生活实践存在着一种张力。研究关注了城市边缘群体日常生活对地理意义的主动塑造过程,对全面理解并规范管理中国城市流浪乞讨现象具有借鉴意义。     

Detection of the end point temperature of thermal denatured protein in fish and chicken meat through SDS-PAGE electrophoresis

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated he...     

Numerical simulation of water flooding into a damaged vessel’s compartment by the volume of fluid method

When a vessel is damaged, seawater floods into the damaged compartments and subsequently influences the motion of the vessel. Furthermore, the vessel’s behaviour affects the floodwater motion. In this paper, a Navier-Stokes (NS) solver with a free surface capturing technique, i.e., the volume of fluid (VOF) method, was developed to numerically simulate water flooding into a damaged vessel. To verify the developed solver, a 2-D and a 3-D dam break problems were tested. The numerical results coincide well with the experimental results and with the published numerical results. Additionally, it was used to solve the problems of linear and non-linear liquid sloshing in a hexahedral tank. The numerical results are satisfactory in comparison with the experimental results and analytical solutions. Finally, the phenomenon of water flooding into a damaged compartment of a Ro-Ro ferry was simulated numerically. The computed results are in good agreement with the experimental data.     

Geomechanics and its application in petroleum geology

This paper is a brief account of the essential points of geomechanics initited in China by Prof. J. S. Lee in the 1920's and some important results achieved by some Chinese petroleum geologists in recent years by applying geomechanics to the exploration and prospecting of oil and gas in eastern China.     

云南个旧南部地区元素地球化学特征

在野外调研和大量样品采集分析的基础上， 运用数学地质中对应分析和因子分析两种方法对样品和变量进行分类划分和成因解释。首先，将样品和元素进行综合计算，按第一、二因子轴作出样品－元素因子平面图，在图上划分四个象限，按象限进行分类，探讨其样品－元素分类规律。其次，按象限计算因子载荷矩阵，分析各因子轴所代表的元素共生组成规律和成矿期次。根据上述研究认为第一因子轴（F1）所代表的Sn、Cu、Pb、Cu、Zn、Au、Ag等元素为主成矿元素组合和主成矿期次，而其它因子轴所代表的Mo、W、Bi、Be等元素为次成矿元素组合和次成矿期次。成矿作用是在区域构造应力驱动力的作用下，与花岗岩侵入有关。成矿溶液除来自深源以外，还与周围基性岩体（脉）和围岩密切相关，矿液实质是一种混合矿浆，在上升运移过程中，充填在有利的构造部位和有利 的地层层位中形成矿体。     

滇西菲红超基性岩风化壳铂族元素地球化学行为

近年来在世界不同地区进行了风化过程中铂族元素的地球化学行为研究。作为中国西南部的第一个实例，本文研究了云南西部菲红超基性岩体上发育的红土风化剖面。矿物学和微量元素地球化学研究证明该风化壳是超基性岩风化的结果。文中详细讨论了风化壳的地质、矿物学、红土化和铂族元素地球化学特征。结果表明，风化壳的红土化程度不高，仍处于红色粘土阶段；风化壳上部的表层土壤带和铁质粘土带中铂族元素总量至少富集了3．57－7．87倍，其中Ru和Pd的富集程度较大,Ir的富集程度中等，Pt和Rh的富集程度较小，使得铂族元素的配分模式由基岩的Pt富集型转变为风化壳的Ru-Pt富集型，证明红土化过程中铂族元素发生了分异。