视频数据库技术在大洋资源调查中的应用研究

在我国近年的大洋科学考察中,深海摄像作业作为重要的可视化调查手段之一,在大洋结核、结壳、热液硫化物以及天然气水合物调查中发挥了重要作用,每年航次均取得近百小时的视像资料数据,海量的视频数据包含了大量的地质信息,如何管理获得的大量视像数据以及如何检索、分析、利用这些数据资源,直接关系到航次获得的视像数据在大洋资源评价中的意义和价值.文中从视像数据的特点出发,结合多媒体技术的开发特点,阐述了我国大洋摄像视频数据库的建立、实施技术方案,以达到与同行的技术沟通,提高我国视频数据库技术在大洋调查中的应用效果.     

东营凹陷南斜坡沙四段高频沉积旋回对滩坝砂体的沉积控制作用

在东营凹陷南斜坡的沙四段地层中,常发育典型的湖相滩坝砂体沉积.详细观察表明,这些滩坝砂体在垂向上常发育较为对称的高频基准面上升半旋回和下降半旋回,且对滩坝砂体的沉积过程有明显的控制作用.通过测井曲线的频谱分析,高频基准面的变化与米兰柯维奇轨道气候周期有着密切的关系,而单砂体规模的滩坝砂体在平面展布上又有着明显的对称性.这些都表明滩坝砂体主要沉积在湖平面升降转换的相对稳定期,同生断层活动产生的陡坎或隆起会有助于滩坝砂体的富集.可根据滩坝砂体的垂向沉积序列和组合方式来判断滩坝主体的分布位置.     

椒江口潮间带大型底栖动物群落beta多样性分析

于2007年10月在椒江口共布设6条潮间带采样断面,每条断面在中潮带上区、中区、下区和低潮区各设1站位进行大型底栖动物采样。分析了不同采样站位数与大型底栖动物种数的关系,选用Wilson-Shmidabeta多样性测度方法,对以下3种空间环境梯度下大型底栖动物群落beta多样性进行了研究:(1)断面内垂直环境梯度;(2)河流到海洋的环境梯度;(3)河口两岸间的距离梯度。分析了不同采样站位数对群落beta多样性指数的影响,结果显示:(1)随着采样站位数的增加,物种数呈增加趋势但增速渐慢,同时beta多样性指数呈下降趋势;(2)不同的潮区间隔下的be-ta多样性指数均有显著差异;(3)在北岸和南岸,随着断面间距的增加beta多样性指数均呈升高趋势,而且与各断面的沉积物粒度、油类含量呈显著相关;(4)椒江口为喇叭形河口,随着河口两岸断面距离的增加beta多样性指数呈升高趋势。     

青岛海域小眼绿鳍鱼耳石形态的初步研究

利用2002年6～12月期间采自青岛海域商业渔获的286尾小眼绿鳍鱼(Chelidonichthys spinosus)标本,以耳石长、耳石宽、周长、面积、矩形趋近率、充实度为基本形态学参数,结合其中傅里叶变换获得的形态特征变量,研究了小眼绿鳍鱼幼鱼生长过程耳石形态的逐月变化特征.结果显示,小眼绿鳍鱼耳石为不规则卵圆形或近四边形,背部弧状隆起,腹部相对略平,前部有一明显缺刻,后部常呈锯齿状尖突.小眼绿鳍鱼耳石长、耳石重、耳石面积皆与体长呈显著相关,耳石长宽比与体长不呈显著相关.随着小眼绿鳍鱼生长,该鱼耳石形态特征随月份变化差异显著;经多因子方差分析和判别分析表明,研究期间小眼绿鳍鱼耳石形态可分为3个特征区间:6月份与其他月份间形态差异显著;7～9月耳石形态特征处于变化间状态;10～12月各方面形态特征趋于稳定.     

影响山东热带气旋活动的气候特征与太平洋海温的关系

利用1949～2003年热带气旋年鉴资料,对55年来影响山东热带气旋(TC)活动的气候特征与太平洋海温的关系进行了分析.结果表明:(1)在厄尔尼诺年,影响山东的TC频数较常年明显偏少.厄尔尼诺次年TC频数较常年稍有增加;拉尼娜年影响山东的TC频数较常年显著偏多,增加明显的月份主要是8月和9月,拉尼娜次年,影响山东TC频数偏少.厄尔尼诺事件强度越大,影响山东的TC频数越少;(2)影响山东的TC数和菲律宾以东洋面的海温呈正相关,并具有很好的持续性.影响山东的TC多年,赤道中东太平洋有较强的负距平区,影响山东的TC少年,赤道中东太平洋为正距平区;(3)厄尔尼诺年,影响山东的TC强度偏强.拉尼娜年,影响山东的TC强度明显偏弱.影响山东的TC强度厄尔尼诺年要比拉尼娜年强很多;(4)影响山东TC偏强年在赤道中东太平洋有较强的正距平区,影响山东TC偏弱年在赤道中东太平洋地区有较强的负距平.综上说明赤道中东太平洋的海温高低对影响山东的TC频数和强度有较好的指示作用.     

斑尾复鰕虎鱼群体的形态学比较

对采自丹东、大连、东营、青岛、赣榆、舟山、厦门7个斑尾复鰕虎鱼群体的可量性状和可数性状进行了主成分分析、聚类分析、判别分析和单因子方差分析.主成分分析和聚类分析结果显示东营和赣榆群体为一组,而其它群体为另一组.2组差异极为显著.主成分分析构建了4个主成分,其贡献率分别为41.30%,8.92%,8.08%和7.31%,累计贡献率为65.60%.第一主成分主要受尾柄长/尾柄高、体高/体长、头长/体长和吻至背鳍起点/体长的影响.判别分析也可将两组群体分开,东营和赣榆组与另一组没有误判,对于样本所属海域的判别,其中东营为100%,最低为青岛群体66.7%,综合判别率为85.5%;选取贡献较大的14个特征值建立判别公式,利用判别公式计算各群体的差别准确率为62.1%～100%.对形态特征参数进行单因子方差分析,计算差异系数,根据Mayr等提出的75%规则,认为两组群体已达到亚种水平,而两组群体内的形态差异为种内不同群体间的差异.     

日本(虫寻)和底栖短桨蟹线粒体DNA 12SrRNA基因序列的初步研究

以相应引物对日本和底栖短桨蟹的线粒体 DNA1 2 S r RNA基因片段进行了 PCR扩增和序列测定 ,分析比较了 2种间序列差异。结果表明 :日本 1 2 S r RNA基因片段长度为 41 4 bp,底栖短桨蟹为 41 5bp,2种的 A,T,G,C含量分别为 1 51 bp(36.47% ) ,1 53bp(36.96% ) ,43bp(1 0 .39% ) ,67bp(1 6.1 8% )和 1 50 bp(36.1 4 % ) ,1 55bp(37.35% ) ,44bp(1 0 .60 % ) ,66bp(1 5.90 % )。 2种间共出现了 3个碱基的缺失 /插入和 38bp的序列差异 ,其碱基转换与碱基颠换比约为 2 .1 7。     

ICESat-2激光卫星与光学遥感影像融合水深测量

针对浅海测深的数据特点和应用需求,以我国南海甘泉岛为例,研究了利用ICESat-2(Ice,Cloud,and Land Elevation Satellite-2)激光卫星数据和光学遥感影像开展主被动融合水深测量的方法。首先通过信号点提取、水面/水底识别、水下点折射改正等步骤处理ICESat-2数据,获得水深值,随后以激光点作为控制,计算光学水深反演模型参数,最后由点及面地获取大范围高精度水深。实验表明,甘泉岛区域主被动融合测深中误差优于1.30m,基于激光卫星数据的主被动融合测深方法能够为浅水水深测量提供新手段。     

西太平洋深海海水中乙醛降解菌多样性及群落结构研究

As a major aldehyde pollutant widely existing in industry and our daily life, acetaldehyde is more and more harmful to human health. As characteristic habitat niche, bacteria from deep sea environments are abundant and distinctive in heredity, physiology and ecological functions. Thus, the development of acetaldehyde-degrading bacteria from deep sea provides a new method to harness acetaldehyde pollutant. Firstly, in this study,acetaldehyde-degrading bacteria in the deep sea water of the West Pacific Ocean were enriched in situ and in the laboratory respectively, and then the diversity of uncultured bacteria was studied by using 16 S r RNA genes. Then acetaldehyde-degrading strains were isolated from two samples, including enrichment in situ and enrichment in laboratory samples of deep sea water from the West Pacific Ocean using acetaldehyde as the sole carbon source,and then the ability of acetaldehyde degradation was detected. Our results showed that the main uncultured bacteria of two samples with different enrichment approaches were similar, including Proteobacteria,Actinobacteria, Firmicutes, Cyanobacteria, but the structure of bacterial community were significant different.Four subgroups, α, γ, δ and ε, were found in Proteobacteria group. The γ-Proteobacteria was dominant(63.5%clones in laboratory enriched sample, 75% clones in situ enriched sample). The species belonged to γ-Proteobacteria and their proportion was nearly identical between the two enrichment samples, and Vibrio was the predominant genus(45% in laboratory enriched sample, 48.5% in situ enriched sample), followed by Halomonas(9% in situ enriched sample) and Streptococcus(6% in laboratory enriched sample). A total of 12 acetaldehyde-degrading strains were isolated from the two samples, which belonged to Vibrio, Halomonas,Pseudoalteromonas, Pseudomonas and Bacillus of γ-Proteobacteria. Strains ACH-L-5, ACH-L-8 and ACH-S-12,belonging to Vibrio and Halomonas, have strong ability of acetaldehyde degradation, which could tolerate 1.5 g/L acetaldehyde and degrade 350 mg/L acetaldehyde within 24 hours. Our results indicated that bacteria of γ-Proteobacteria may play an important role in carbon cycle of deep sea environments, especial the bacteria belonging to Vibrio and Halomonas and these strains was suggested for their potentials in government of aldehyde pollutants.     

Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.