Involvement of cyclic electron flow in irradiance stress responding and its potential regulation of the mechanisms in <Emphasis Type="Italic">Pyropia yezoensis</Emphasis>

Pyropia yezoensis, belongs to the genus of Porphyra before 2011, inhabit on intertidal zone rocks where irradiation changes dramatically, implying that the seaweed has gained certain mechanisms to survive a harsh environment. Based on the photosynthetic parameters with or without the inhibitors determined by a Dual-PAM-100 apparatus, we investigated the photosynthetic performance and the changes in electron flow that occurred during the algae were stressed with different light intensities previously. When the irradiation saturation was approaching, the CEF around PS I became crucial since the addition of inhibitors usually led to an increase in non-photochemical quenching. The inhibitor experiments showed that there were at least three different CEF pathways in Py. yezoensis and these pathways compensated each other. In addition to maintaining a proper ratio of ATP/NAD(P)H to support efficient photosynthesis, the potential roles of CEF might also include the regulation of different photoprotective mechanisms in Py. yezoensis. Under the regulation of CEF, chlororespiration is thought to transport electrons from the reduced plastoquinone (PQ) pool to oxygen in order to mitigate the reduction in the electron transfer chain. When irradiation was up to the high-grade stress conditions, the relative value of CEF began to decrease, which implied that the NADP⁺ pool or PQ⁺ pool was very small and that the electrons were transferred from reduced PS I to oxygen. The scavenging enzymes might be activated and the water-water cycle probably became an effective means of removing the active oxygen produced by the irradiation stressed Py. yezoensis. We believe that the different mechanisms could make up the photoprotective network to allow Py. yezoensis for survival in a highly variable light stress habitat, which may enlighten scientists in future studies on irradiance stress in other algae species.     

Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

Numerical study on inter-tidal transports in coastal seas

Inter-tidal(subtidal) transport processes in coastal sea depend on the residual motion, turbulent dispersion and relevant sources/sinks. In Feng et al.(2008), an updated Lagrangian inter-tidal transport equation, as well as new concept of Lagrangian in- ter-tidal concentration(LIC), has been proposed for a general nonlinear shallow water system. In the present study, the LIC is nu- merically applied for the first time to passive tracers in idealized settings and salinity in the Bohai Sea, China. Circulation and tracer motion in the three idealized model seas with different topography or coastline, termed as ‘flat-bottom', ‘stairs' and ‘cape' case, re- spectively, are simulated. The dependence of the LIC on initial tidal phase suggests that the nonlinearities in the stairs and cape cases are stronger than that in the flat-bottom case. Therefore, the ‘flat-bottom' case still meets the convectively weakly nonlinear condi- tion. For the Bohai Sea, the simulation results show that most parts of it still meet the weakly nonlinear condition. However, the de- pendence of the LIS(Lagrangian inter-tidal salinity) on initial tidal phase is significant around the southern headland of the Liaodong Peninsula and near the mouth of the Yellow River. The nonlinearity in the former region is mainly related to the complicated coast- lines, and that in the latter region is due to the presence of the estuarine salinity front.     

基于Coons曲面的似大地水准面精化算法研究

针对线状或带状GPS水准点控制的似大地水准面,提出Coons曲面内插模型。先将GPS水准点拟合成曲线,再通过曲线构造曲面,尽可能减少传统拟合算法由点直接推面的精度消耗。分别运用最小二乘法、最小二乘配置法、移动内插法和Coons曲面法对线状GPS水准点控制的似大地水准面进行拟合,结果表明,基于Coons曲面的内插模型算法精度最高。     

ɽ��������ش���������1999-2008��ؿ������ܶȱ仯

???????????????????????????????????????????????????1999-2008????????????仯??????????????????????????????????????????????仯??????????????????????????в??????????????????????????????????????????????????????????     

Spermatozoid life-span of two brown seaweeds, Saccharina japonica and Undaria pinnatifida, as measured by fertilization efficiency

During sexual reproduction of seaweeds, spermatozoid (sperm) discharge is triggered by chemical messengers (pheromones) released by the female gametes. The chemotactic ability of the sperm ensures fertilization success. Using unialgal male and female gametophyte material under designated standard gametogenesis testing (SGT) conditions, the potential life-span of the sperm of two seaweeds, Saccharina japonica and Undaria pinnatifida, was assessed by their ability to fertilize eggs. Results show that within 20-30 min after being discharged, sperm of both species could complete fertilization without an apparent decline in fertilization rate. Although fertilization rate 60–120 min after sperm discharge dropped significantly in both species, some sperm were viable enough to fertilize the eggs. In S. japonica, at 12°C, some sperm were able to fertilize eggs up to 12 h after discharge. In both species, egg discharge rates (EDR) in the male and female mixed positive controls were significantly higher than those of all the sperm-testing groups. Doubling the seeded male gametophytes of S. japonica in the SGT tests significantly increased the EDR, further confirming the effect of the presence of the male on the female in terms of facilitating egg discharge from oogonia.     

Porosity Distribution Characteristics and Geological Analysis of Brine Storage Medium in the Qaidam Basin,Western China

Underground brine is an unusual water resource that contains abundant mineral resources. It is distributed widely in the Qaidam Basin, western China, a hyperarid inland basin located in the northern Tibetan Plateau. Pores in the brine storage medium act as storage space and transmission channels of underground brine. Therefore, the porosity of brine storage medium determines its ability to store brine. In this study, Mahai Salt Lake was used as the research area as a modern saline lake located in the north area of the Qaidam Basin. A total of 100 porosity samples were collected from eight sampling points in two profiles of the research area at sampling depths of 1.30–314.78 m. The porosity distribution characteristics and influencing factors in brine storage medium were analysed according to the measured porosity data. Based on analysis of the pore structure characteristics, the brine storage medium contains intercrystalline pores, unlike conventional freshwater storage mediums. Moreover, the primary salt rock is susceptible to dissolution by lighter brine, facilitating the formation of secondary porosity. Due to the formation of secondary pores, a porosity greater than 20% remains even at buried depths greater than 100 m. Based on the geological statistical analysis, due to the geographic location, salt formation time, and depositional environment, the porosity values of Mahai Salt Lake do not exhibit a wider distribution, but also show more extreme values than a nearby salt lake. Based on the porosity characteristics by depth, due to the presence of secondary pores, flooding, stratigraphic static pressure, and other factors, porosity shows fluctuations with increasing depth.     

我国恐龙空间分异原因初探

本文讨论了中国境内先后出现的恐龙动物在古地理位置、分布范围上的变化特点，提出引起中国恐龙空间分异的主要原因是占地理环境的巨大变迁，同时也与占气候、占植物的变化有密切关系。并进一步指出，中国恐龙绝灭的原因应该是突发的灾变。     

基于拓扑与形态特征的城市道路交通状态空间自相关分析

城市道路交通状态具有空间自相关特征。某一道路交通状态的变化会对其周边道路产生影响,故把握道路交通状态的空间自相关性是提高交通规划、交通预测水平的基础。然而,城市道路交通状态又具有空间异质性,即道路交通状态的影响扩散并非各向同性,其使得道路交通状态空间自相关性的度量更为复杂,因此仅从地理空间下道路之间的邻近关系出发进行分析有失偏颇。同时,城市道路具有拓扑结构特征和几何形态特征,二者对于交通状态自相关性的影响和制约,却未引起足够重视。本文从城市道路的拓扑结构特征和几何形态特征出发,提出了一种新的交通状态空间自相关路段识别规则,即基于交通状态变化的路段空间识别规则,通过拓扑社区发现方法刻画路段在空间上的聚集特征,同时,基于Stroke跟踪的几何形态概化来描述道路交通状态变化影响的空间异质性。结果表明,利用本文提出的识别规则产生的交通状态自相关路段集合,较仅考虑地理空间邻近或拓扑结构的识别规则更为合理,更好地揭示了城市道路交通状态的空间自相关特征。     

Southern high latitude climate anomalies associated with the Indian Ocean dipole mode

1 INTRODUCTION In southern high latitudes, recent observations have shown a standing mode of ACW (Antarctic Circumpolar Wave) with eastward propagation across the Southern Ocean of the Antarctic in co- varying SST (sea surface temperature) and SLP (sea le…