Development of three multiplex PCR primer sets for ark shell (<Emphasis Type="Italic">Scapharca broughtonii</Emphasis>) and their validation in parentage assignment

Scapharca broughtonii is a commercially important and over-exploited species. In order to investigate its genetic diversity and population structure, 43 novel polymorphic microsatellites were isolated and characterized. The number of alleles per locus ranged from 3 to 22 with an average of 6.93, and the observed and expected heterozygosities varied between 0.233 and 1.000, and 0.250 and 0.953, with an average of 0.614 and 0.707, respectively. Three highly informative multiplex PCRs were developed from nine of those microsatellites for S. broughtonii. We evaluated and validated these multiplex PCRs in 8 full-sib families. The average polymorphism information content (PIC) was 0.539. The frequency of null alleles was estimated as 3.13% of all the alleles segregation based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 100% of all offspring were unambiguously allocated to a pair of parents based on 3 multiplex sets. Those 43 microsatellite loci with high variability will be helpful for the analysis of population genetics and conservation of wild stock of S. broughtonii. The 3 sets of multiplex PCRs could be an important tool of pedigree reconstruction, population genetic analysis and brood stock management.     

基于主成分分析的济宁市环境承载力研究

该文以济宁市环境承载力为研究对象,运用主成分分析法,从影响环境污染状况、环境治理状况、人口状况、经济发展状况、自然资源状况、物耗能耗状况等19个指标中,提取出影响环境承载力动态变化的3个主成分,对济宁市环境承载力年际变化进行了综合评价。结果表明:2006—2010年,济宁市环境承载力呈现逐年上升的趋势。该研究评价结果较好,可以为该地区的可持续开发规划提供理论参考。     

基于多元对应分析的国家地质公园综合价值及分类

目前地质公园的分类难以针对具有多重价值属性的各类地质公园提出行之有效的分类管理模式。提出基于综合价值的国家地质公园分类模式,建立了国家地质公园综合价值评价指标,通过问卷调查与小组讨论获取了35个国家地质公园的量化综合价值评价指标数据,应用多元对应分析对量化数据进行统计处理,最后将35个国家地质公园分为禀赋型地质公园、生态型地质公园、成熟型地质公园、均衡型地质公园和发展型地质公园五大类,为国家地质公园分类管理提供依据。     

海洋重力测量数据精度分析

海洋重力测量仪器工作环境的特殊性,决定了其测量数据精度分析与检测难以方便实现。在分析一般数据处理方法的基础上,研究了海洋重力测量精度分析的特点,采用一种以重复测线不符值、测线网交叉点不符值的标准差对海洋重力测量的内符合精度进行分析,并根据重复测线、交叉点内符合精度评估方法,通过对某型海洋重力测量仪器的多次航行试验,获取了该型仪器测量数据的部分精度数据,分析结果表明该方法可以全面衡量仪器性能。     

脐带缆在潜器下放过程中的运动建模与仿真

潜器在下放布置过程中需要考虑变长度的脐带缆对其运动的影响,在考虑流的情况下,对脐带缆的有限差分模型进行了改进,并采用最小二乘方法求解由时间和空间上的中心差分格式离散后的非线性方程组。为了验证模型改进的有效性,将潜器在均匀定常流中水平匀速直航时的数值解与该特殊情况下的解析解进行比较,两组解的吻合不仅证实了模型改进的有效性,而且表明上述数值计算方法是可靠且有效的。在潜器下放布置运动给定的情况下,脐带缆的运动仿真结果表明,潜器姿态角的调整会对拖曳点处的缆绳张力大小和变化趋势产生显著的影响,流在改变缆绳空间形状的同时引起了缆绳对潜器作用力和力矩的非线性和时变性,脐带缆总是阻止潜器自身动力对运动速度和姿态角的改变,且对纵荡运动的影响最大。     

A Preliminary Study on the Soft–Sediment Deformation Structures in the Late Quaternary Lacustrine Sediments at Tashkorgan, Northeastern Pamir, China

This study identified soft–sediment deformation structures(SSDS) of seismic origin from lacustrine sediments in the late Quaternary paleo–dammed lake at Tashkorgan, northeastern Pamir. The observed deformation structures include sand dykes, liquefied diapir and convolute structures, gravity induced SSDS, and thixotropic pillar and tabular structures. We conducted a preliminary study on the morphology, formation and trigger mechanisms of pillar and tabular structures formed by liquefaction of underlying coarse sand and thixotropy of the upper silty clay. The regional tectonic setting and distribution of lacustrine strata indicate that the most probable trigger for the SSDS in lacustrine sediments was seismic activity, with an approximate earthquake magnitude of M6.0; the potential seismogenic fault is the southern part of the Kongur normal fault extensional system. AMS 14 C dating results indicate that the SSDS were formed by seismic events occurring between 26050±100 yr BP and 22710±80 yr BP, implying intense fault activity in this region during the late Pleistocene. This study provides new evidence for understanding tectonic activity and regional geodynamics in western China.     

Provenance Study of Fe–Ti Oxide Minerals in the Quaternary Sediments in Yichang Area and Its Implication of Formation Time of the Yangtze Three Gorges, China

The Three Gorges are considered to be critical to understand the formation of Yangtze River. Recent research results suggest that the Yangtze Three Gorges was created during the Quaternary but the exact time is debatable. Fe–Ti oxide minerals are seldom used to study sediment provenance, expecially using scanning electron microscopy(SEM), and energy dispersive spectrometer(EDS). In this study, the provenance of Quaternary sediments in Yichang area, which is located to the east of the Yangtze Three Gorges, was investigated by using SEM and EDS to research Fe–Ti oxides. The Panzhihua vanadium titanomagnetite and Emeishan basalt outcrop are located to the west of the Three Gorges. Further, the materials from them are observed in the Quaternary sediments of Yichang area. Fe–Ti oxide minerals from the Huangling granite are observed in the Yunchi and Shanxiyao Formations, which were formed before 0.75 Ma B.P., whereas Fe–Ti oxide minerals from the Huangling granite, Panzhihua vanadium titanomagnetite, and Emeishan basalt are observed in the riverbed and fifth-terrace sediments of the Yangtze River, which were formed after 0.73 Ma B.P.. Thus, we can infer that the Three Gorges formed after the deposition of the Shanxi Formation and before the fifth-terrace; i.e., 0.75–0.73 Ma B.P..