A search for amino acids and nucleobases in the Martian meteorite Roberts Massif 04262 using liquid chromatography‐mass spectrometry

The investigation into whether Mars contains signatures of past or present life is of great interest to science and society. Amino acids and nucleobases are compounds that are essential for all known life on Earth and are excellent target molecules in the search for potential Martian biomarkers or prebiotic chemistry. Martian meteorites represent the only samples from Mars that can be studied directly in the laboratory on Earth. Here, we analyzed the amino acid and nucleobase content of the shergottite Roberts Massif (RBT) 04262 using liquid chromatography‐mass spectrometry. We did not detect any nucleobases above our detection limit in formic acid extracts; however, we did measure a suite of protein and nonprotein amino acids in hot‐water extracts with high relative abundances of β‐alanine and γ‐amino‐n‐butyric acid. The presence of only low (to absent) levels of several proteinogenic amino acids and a lack of nucleobases suggest that this meteorite fragment is fairly uncontaminated with respect to these common biological compounds. The distribution of straight‐chained amine‐terminal n‐ω‐amino acids in RBT 04262 resembled those previously measured in thermally altered carbonaceous meteorites (Burton et al. 2012; Chan et al. 2012). A carbon isotope ratio of ?24‰ ± 6‰ for β‐alanine in RBT 04262 is in the range of reduced organic carbon previously measured in Martian meteorites (Steele et al. 2012). The presence of n‐ω‐amino acids may be due to a high temperature Fischer‐Tropsch‐type synthesis during igneous processing on Mars or impact ejection of the meteorites from Mars, but more experimental data are needed to support these hypotheses.     

Long-term deforestation in NW Spain: linking the Holocene fire history to vegetation change and human activities

The Holocene fire regime is thought to have had a key role in deforestation and shrubland expansion in Galicia (NW Spain) but the contribution of past societies to vegetation burning remains poorly understood. This may be, in part, due to the fact that detailed fire records from areas in close proximity to archaeological sites are scarce. To fill this gap, we performed charcoal analysis in five colluvial soils from an archaeological area (Campo Lameiro) and compared the results to earlier studies from this area and palaeo-ecological literature from NW Spain. This analysis allowed for the reconstruction of the vegetation and fire dynamics in the area during the last ca 11 000 yrs. In the Early Holocene, Fabaceae and Betula sp. were dominant in the charcoal record. Quercus sp. started to replace these species around 10 000 cal BP, forming a deciduous forest that prevailed during the Holocene Thermal Maximum until ～5500 cal BP. Following that, several cycles of potentially fire-induced forest regression with subsequent incomplete recovery eventually led to the formation of an open landscape dominated by shrubs (Erica sp. and Fabaceae). Major episodes of forest regression were (1) ～5500–5000 cal BP, which marks the mid-Holocene cooling after the Holocene Thermal Maximum, but also the period during which agropastoral activities in NW Spain became widespread, and (2) ～2000–1500 cal BP, which corresponds roughly to the end of the Roman Warm Period and the transition from the Roman to the Germanic period. The low degree of chronological precision, which is inherent in fire history reconstructions from colluvial soils, made it impossible to distinguish climatic from human-induced fires. Nonetheless, the abundance of synanthropic pollen indicators (e.g. Plantago lanceolata and Urtica dioica) since at least ～6000 cal BP strongly suggests that humans used fire to generate and maintain pasture.     

Correlation between the structural pattern and the development of the hydrographic network in a portion of the Western Thessaly Basin (Greece)

In the context of the present study the structural pattern in the Western Thessaly Basin (Greece) has been examined, based on structural data collected from the entire study area that were further correlated to the hydrographic network. The structural pattern of the area was revealed from tectonic analysis. Additionally, the topography, stratigraphy and sedimentology of the study area were taken into account. GIS techniques were used to map the spatial distribution of the geological and tectonic features on the topographic relief of the area. The oldest structures are contractional in nature, deformed by normal faulting related to the extensional episodes initiated in Serravallian times. It is inferred that the orientation of the stress field in the area has changed several times: the N-S stress field which was dominant during Late Serravallian times changed to NW-SE (Messinian-Zanclian) up to E-W in Zanclian and subsequently to roughly NNW-SSE (in late Piacencian). The NE-SW stress that was dominant in Pleistocene became N-S in later times. In addition, some changes in orientation are also indicated for the transitional periods of the pre-mentioned extensional episodes, possibly related to local events, or as a block-related deformation. The development of the 7^th order streams is probably related to the N-S extensional faulting initiated in Pliocene times, while the dominant direction of the 6^th, 5^th and 4^th order streams is possibly connected with the presence of the NNE-SSW and NW-SE extensional faults. Finally, the lower order streams are probably related to the most recent E-W striking normal faults.     

Investigation of waste disposal areas using electrical methods: a case study from Chania,Crete, Greece

A geophysical investigation of a landfill area was conducted as inextricable phase of a preliminary geotechnical, geological and hydrogeological study of the area under investigation. Electrical resistivity tomography (ERT), one of the most promising prospecting techniques mainly concerning its effective contribution to resolve several environmental problems, was applied for the geophysical modeling. ERT is a robust imaging method the theory and implementation of which are well documented in geophysical research literature. The survey site is located in Akrotirion Peninsula, about 7 km east of Chania city in Crete I. The geological setting comprises Trypalion limestones, marly limestones and a clayed weathered layer. Cavities are also identified at various scales which are recent tectono-karstic voids. Due to the above-mentioned geological singularity and in the light of the requirement for an environmentally safe construction of the landfill, an ERT survey was carried out. Specifically, seven geoelectrical tomographies were conducted reaching the prospecting depth of 36 m. The resulted images conduced, to overcome geotechnical problems since the spatial distribution of karstic voids was determined, to plan the future sites for waste disposal as the geological conditions were studied in detail and to reliably estimate the thickness of the already deposited wastes. The resulted images were confirmed using available borehole logs and ambient noise measurements.     

Testing optically stimulated luminescence dating on sand-sized quartz of deltaic deposits from the Sperchios delta plain,central Greece

This study reports on the first investigation into the potential of luminescence dating to establish a chronological framework for the depositional sequences of the Sperchios delta plain, central Greece. A series of three borehole cores(20 m deep) and two shallow cores(4 m deep), from across the delta plain, were extracted, and samples were collected for luminescence dating. The luminescence ages of sand-sized quartz grains were obtained from small aliquots of quartz, using the Single-Aliquot Regenerative-dose(SAR) protocol.The equivalent dose determination included a series of tests and the selection of the Minimum Age Model(MAM) as the most appropriate statistical model. This made it possible to confirm the applicability of quartz Optically Stimulated Luminescence(OSL) dating to establish absolute chronology for deltaic sediments from the Sperchios delta plain.Testing age results of the five cores showed that the deltaic sediments were deposited during the Holocene.A relatively rapid deposition is implied for the top ~14 m possibly as a result of the deceleration in the rate of the sea-level rise and the transition to terrestrial conditions, while on the deeper parts, the reduced sedimentation rate may indicate a lagoonal or coastal environment.     

Seawater nutrient and carbonate ion concentrations recorded as P/Ca, Ba/Ca, and U/Ca in the deep-sea coral Desmophyllum dianthus

As paleoceanographic archives, deep sea coral skeletons offer the potential for high temporal resolution and precise absolute dating, but have not been fully investigated for geochemical reconstructions of past ocean conditions. Here we assess the utility of skeletal P/Ca, Ba/Ca and U/Ca in the deep sea coral D. dianthus as proxies of dissolved phosphate (remineralized at shallow depths), dissolved barium (trace element with silicate-type distribution) and carbonate ion concentrations, respectively. Measurements of these proxies in globally distributed D. dianthus specimens show clear dependence on corresponding seawater properties. Linear regression fits of mean coral Element/Ca ratios against seawater properties yield the equations: P/Ca_coral (μmol/mol) = (0.6 ± 0.1) P/Ca_sw(μmol/mol) - (23 ± 18), R² = 0.6, n = 16 and Ba/Ca_coral(μmol/mol) = (1.4 ± 0.3) Ba/Ca_sw(μmol/mol) + (0 ± 2), R² = 0.6, n = 17; no significant relationship is observed between the residuals of each regression and seawater temperature, salinity, pressure, pH or carbonate ion concentrations, suggesting that these variables were not significant secondary dependencies of these proxies. Four D. dianthus specimens growing at locations with Ω_arag ? 0.6 displayed markedly depleted P/Ca compared to the regression based on the remaining samples, a behavior attributed to an undersaturation effect. These corals were excluded from the calibration. Coral U/Ca correlates with seawater carbonate ion: U/Ca_coral(μmol/mol) = (−0.016 ± 0.003) (μmol/kg) + (3.2 ± 0.3), R² = 0.6, n = 17. The residuals of the U/Ca calibration are not significantly related to temperature, salinity, or pressure. Scatter about the linear calibration lines is attributed to imperfect spatial-temporal matches between the selected globally distributed specimens and available water column chemical data, and potentially to unresolved additional effects. The uncertainties of these initial proxy calibration regressions predict that dissolved phosphate could be reconstructed to ±0.4 μmol/kg (for 1.3-1.9 μmol/kg phosphate), and dissolved Ba to ±19 nmol/kg (for 41-82 nmol/kg Ba_sw). Carbonate ion concentration derived from U/Ca has an uncertainty of ±31μmol/kg (for ). The effect of microskeletal variability on P/Ca, Ba/Ca, and U/Ca was also assessed, with emphasis on centers of calcification, Fe-Mn phases, and external contaminants. Overall, the results show strong potential for reconstructing aspects of water mass mixing and biogeochemical processes in intermediate and deep waters using fossil deep-sea corals.     

Estimation of the Strength of Stratified Rock Mass

Stratified rock formations are often encountered in engineering structures with large dimensions, such as tunnels, caverns, etc. In the present work, the question of how and when such materials fail is addressed. It is argued that average values cannot provide appropriate measures for the strength of layered rock, as local stresses and strains differ greatly in the various constituents and one may fail, while others remain intact. The present work attempts, therefore, to formulate a solution covering both elasticity and failure in three dimensions, though simple enough to be easy to use in engineering applications. To this end, the local stress and strain state in each constituent were evaluated, assuming the constituents to be orthotropically elastic up to the point of failure. Subsequently, several failure criteria are considered and an example is presented to illustrate the model’s response. A discussion on the effect of geometry and on the values of local versus global quantities follows.     

Radio imaging of the Subaru/XMM–Newton Deep Field – II. The 37 brightest radio sources

We study the 37 brightest radio sources in the Subaru/ XMM–Newton Deep Field. We have spectroscopic redshifts for 24 of 37 objects and photometric redshifts for the remainder, yielding a median redshift z _med for the whole sample of   z _med≃ 1.1  and a median radio luminosity close to the 'Fanaroff–Riley type I/type II (FR I/FR II)' luminosity divide. Using mid-infrared (mid-IR) ( Spitzer MIPS 24 μm) data we expect to trace nuclear accretion activity, even if it is obscured at optical wavelengths, unless the obscuring column is extreme. Our results suggest that above the FR I/FR II radio luminosity break most of the radio sources are associated with objects that have excess mid-IR emission, only some of which are broad-line objects, although there is one clear low-accretion-rate object with an FR I radio structure. For extended steep-spectrum radio sources, the fraction of objects with mid-IR excess drops dramatically below the FR I/FR II luminosity break, although there exists at least one high-accretion-rate 'radio-quiet' QSO. We have therefore shown that the strong link between radio luminosity (or radio structure) and accretion properties, well known at z ∼ 0.1, persists to z ∼ 1. Investigation of mid-IR and blue excesses shows that they are correlated as predicted by a model in which, when significant accretion exists, a torus of dust absorbs ∼30 per cent of the light, and the dust above and below the torus scatters ≳1 per cent of the light.     

A micromechanically derived anisotropic micropolar constitutive law for granular media: Elasticity

In the present work, it is attempted to derive a macroscopic constitutive law for the elastic deformation of granular materials, based on microscopic considerations. For the sake of simplicity, the solution is restricted to two dimensions, that is, a random assembly of infinitely extended cylinders. After examining pairwise contact interactions, the elastic energy rate of the assembly is retrieved in a discrete form. Introducing the probability density function of the contact orientations, the continuum form of the elastic energy density rate is evaluated as a function of generalized strains and curvatures, and their rates. The stresses and couple stresses result as dual variables to the generalized strain and curvature rates. Some properties of the resulting model are discussed, examples are presented and conclusions are drawn.Copyright © 2014 John Wiley & Sons, Ltd.