日本凋毛藻碳酸酐酶基因及其编码的蛋白特征

碳酸酐酶CA(EC 4.2.1.1)是含锌原子的金属蛋白酶,该酶的基本功能是催化CO₂与HCO₃-间的相互转换[1].该反应在许多生物过程中都发挥着重要的作用,例如离子交换、呼吸作用、pH稳定性、捕获CO₂和光合作用等[2~5].CA广泛分布于各种生物中,已知的CA酶根据氨基酸序列被分为-αCA,-βCA和γ-CA三种类型[5~7].这3种类型的CA酶序列间的相似性很低,被认为是由不同的途径进化而来的[7].α-型的CA酶在动物、植物、绿藻、细菌和蓝细菌中都有发现[7],在藻类中报道的α-型CA还很少.迄今为止只在衣藻中发现2种异构体,在盐藻中发现3种[8,9],在红藻中还未见报道.     

海洋环境立体监测系统的设计方法

“九五”和“十五”期间,在国家海洋863计划的支持下,我国分别在上海和台湾海峡及毗邻海域建立了两个区域性海洋环境立体监测示范系统。在这两个示范系统的技术研究成果的基础上,通过分析国际海洋立体监测系统的设计模型,提出了区域性海洋环境立体监测系统的设计原则和设计方法,包括:系统结构、数据与信息服务通信网络、数据处理中心、数据流程以及系统功能等方面的设计方法。     

Multivariate analysis of fluorescence and source identification of dissolved organic matter in Jiaozhou Bay, China

Hierarchical clustering analysis and principal component analysis (PCA) methods were used to assess the similarities and dissimilarities of the entire Excitation-emission matrix spectroscopy (EEMs) data sets of samples collected from Jiaozhou Bay, China. The results demonstrate that multivariate analysis facilitates the complex data treatment and spectral sorting processes, and also enhances the probability to reveal otherwise hidden information concerning the chemical characteristics of the dissolved organic matter (DOM). The distribution of different water samples as revealed by multivariate results has been used to track the movement of DOM material in the study area, and the interpretation is supported by the results obtained from the numerical simulation model of substance tracing technique, which show that the substance discharged by Haibo River can be distributed in Jiaozhou Bay.     

Tidal inlet sequence, Sundance Formation (Upper Jurassic), north-central Wyoming

The sandstones and coquinas of the upper 20 m of the Sundance Formation are interpreted as a tidal inlet, back-barrier shoal and sandy tidal-flat sequence deposited at the close of marine Jurassic sedimentation in north-central Wyoming. The barrier strandline maintained a generally E-W trend as it prograded to the north. The lateral migration of inter-barrier tidal inlets along the regressive shoreline of the late Sundance sea caused the coquinas and sandstones of the uppermost Sundance Formation to be deposited as tabular, laterally-extensive units. Tidal bundles, sigmoidal reactivation surfaces, herringbone cross-lamination and abundant mud drapes within the sandstones are evidence of considerable tidal influence during the deposition of the uppermost Sundance Formation. Earlier models, which attach an offshore environment of deposition to the sequence, do not explain the tabular geometries of the sandstone and coquina units and their conformable stratigraphic relationship with the overlying non-marine sediments of the Morrison Formation.     

TRANSIENT EM ANALOGUE MODELLING FOR KOREAN TREASURE HUNTING WITH THE SIROTEM SYSTEM1

The inductive transient electromagnetic method (TEM) shows good potential for the detection of metallic relics of historical interest, such as buried antique bells, Buddhist idols, or precious metal nuggets. The effectiveness of the method was investigated with analogue models, using transmitter-receiver loop configurations with sizes or receiver spacings slightly different from those usually applied in earth resources exploration with the Sirotem system. The analogue modelling results show that the location and depth of the buried treasure may be obtained from the Sirotem data. A solid metallic object such as an antique bell could be detected to a depth about 12 times greater than its size, and treasure consisting of separate metallic objects, such as gold nuggets, could be detected to a depth 5 times its linear dimensions with a transmitter current of ? 20 A. In scaling down the dimensions of a target, its conductivity should be increased in order to preserve the same TEM conditions found in the field. However, since the buried treasure consists of gold or copper objects, it is not possible to properly scale the conductivity. Hence, in the field, the depth detection limit is expected to be greater than that derived from analogue modelling.     

Combined flow origin of edgewise intraclast conglomerates: Sellick Hill Formation (Lower Cambrian), South Australia

Unusually thick, coarse grained edgewise intraclast conglomerates occur at eight or more horizons within subtidal nodular and ribbon bedded wackestones and packstones of the Lower Cambrian Sellick Hill Formation, South Australia. The intraclast beds are flat based and laterally discontinuous, forming bar-like structures that must have exhibited bathymetric relief of as much as 1 m. The internal fabrics of these beds are variable. Thinner beds are dominated by flat-lying intraclasts; thicker beds contain both chaotic, randomly oriented, steeply inclined intraclasts and clusters of fan-shaped, vertically stacked edgewise intraclasts. The Sellick Hill Formation intraclast conglomerates are inferred to have been formed by intense, storm-generated combined flows on a broad, subtidal carbonate ramp. Superimposition of wave-induced oscillatory motions on geostrophic bottom flows during large storms generates short-lived, but exceptionally high instantaneous shear stresses in the bottom boundary layer. Entrainment of the relatively large intraclasts occurs through sliding, rather than pivoting. Edgewise fabrics are a product of asymmetric acceleration and deceleration of intraclasts during passage of waves and the chaotic nature of collisions between intraclasts moving within the boundary layer. Collisions between intraclasts impart a rotating moment, causing intraclasts to tip up during maximum fluid shear stress. Lodgement or packing of clasts in vertical or steeply inclined positions occurs within scours, where intraclasts can wedge between other vertically inclined clasts, or where intraclasts are pinned in steep orientations by collisions with shallowly inclined intraclasts. Differential erosional resistance of the intraclast deposits probably led to the development of sharp lateral changes in thickness. The Sellick Hill Formation intraclast conglomerates record erosion and reworking of subtidal, subfairweather wave base environments by exceptionally intense and presumably rare storm flows. The intraclast horizons represent a substantial loss in stratigraphic resolution due to widespread erosion of the ramp.     

Planned Shopping Centres

Planned shopping centres affect the geographical direction of retailing and the nature of retailing in general. As major marketing outlets for national and international chains they are especially attractive to consumers. This paper examines shopping centres in New Zealand and their impact on stand alone retailers and includes a case study of the Queensgate shopping centre in Lower Hutt.     

Transform-normal extension on the Northern Death Valley fault system, California-Nevada

The Northern Death Valley fault zone is a major right-lateral structure that has accommodated 70 km or more of regional transtensional deformation in Tertiary to Recent time. Extension parallel to its north-west transport direction in the Death Valley region of California has produced ‘pull-apart’ structures that are responsible for opening the central Death Valley rhombochasm. In several ranges along the length of the Northern Death Valley fault zone, there is also evidence for extension directed to the south-west, normal to strike-slip movement. Evidence from the Funeral, Grapevine and Cottonwood Mountains suggests that a significant amount of down-dip slip has occurred on the Northern Death Valley fault zone and parallel structures (together referred to as the Northern Death Valley fault system) coeval with the majority of right-lateral slip and transform-parallel extension. As a result of both these components of extension, a separate basin opened in northern Death Valley with an orientation and architecture very different from that of central Death Valley. In addition, the Northern Death Valley fault system may be responsible for the present topography of the Funeral and Grapevine Mountains. Transform-normal extension appears to be the result of a misorientation of the Northern Death Valley fault zone within the regional stress field over the past 6 Myr, as suggested by simple geometric calculations.     

Ripple formation in combined transdirectional steady and oscillatory flow

Measurements are described of the geometry of ripples formed on beds of sand exposed to a steady current at right angles to an oscillatory flow. Four different sands were studied. The oscillation was produced by an oscillating tray set into the bed of a steady-flow flume. It was observed that straight-crested ripples formed by oscillatory flow would usually develop a ‘serpentine’ form when the superimposed steady current exceeded a certain limit. For amplitudes of the tray velocity U_∞ less than about 0.38 m s^-1 this limit corresponded to U_∞/ū_*c>31, where ū_*c is the shear velocity measured just upstream of the oscillating tray. It is suggested that the serpentine form is caused by the interaction of vortices carried back and forth between adjacent ripples. On this assumption, the wavelength of the serpentine form would be proportional to the product of period of oscillation and near-bed steady current velocity. The present measurements appear to support this hypothesis although there is also evidence that the wavelength is influenced by preferred spacing patterns between vortices. The measurements also show the ratio of the amplitude of the serpentine form to its wavelength to be approximately constant. Empirical relationships are derived relating ripple geometry to flow and sediment properties. It is observed that the influence of Reynolds number and sediment properties on the geometry is very weak. It is suggested that this is typical of ripples formed with relatively low sediment transport rates. It is also found that, under the present experimental conditions, the ripple spacing in the direction of oscillation is almost independent of the magnitude of the steady current and in close agreement with the wavelengths previously measured in an oscillating water tunnel. This suggests that the additional inertia effects associated with oscillating tray rigs were not sufficient to affect bed geometry under the present test conditions.